Gene description for KCTD13
Gene name potassium channel tetramerization domain containing 13
Gene symbol KCTD13
Other names/aliases BACURD1
FKSG86
PDIP1
POLDIP1
hBACURD1
Species Homo sapiens
 Database cross references - KCTD13
ExoCarta ExoCarta_253980
Vesiclepedia VP_253980
Entrez Gene 253980
HGNC 22234
MIM 608947
UniProt Q8WZ19  
 KCTD13 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for KCTD13
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IBA
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 IEA
    small GTPase binding GO:0031267 IDA
    identical protein binding GO:0042802 IPI
Biological Process
    cell migration GO:0016477 IMP
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 IDA
    protein ubiquitination GO:0016567 IEA
    negative regulation of Rho protein signal transduction GO:0035024 IBA
    negative regulation of Rho protein signal transduction GO:0035024 IMP
    negative regulation of Rho protein signal transduction GO:0035024 ISS
    stress fiber assembly GO:0043149 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    positive regulation of DNA replication GO:0045740 IEA
    positive regulation of synaptic transmission GO:0050806 ISS
    protein homooligomerization GO:0051260 IEA
    neural precursor cell proliferation GO:0061351 ISS
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 TAS
    nuclear body GO:0016604 IDA
    Cul3-RING ubiquitin ligase complex GO:0031463 IBA
    Cul3-RING ubiquitin ligase complex GO:0031463 IDA
 Experiment description of studies that identified KCTD13 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for KCTD13
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZMYND19  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 LAMB4  
Affinity Capture-MS Homo sapiens
3 SPRTN  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 GFPT2 9945
Affinity Capture-MS Homo sapiens
5 USP25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 PLEC 5339
Affinity Capture-MS Homo sapiens
7 KCTD10 83892
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SRPX 8406
Affinity Capture-MS Homo sapiens
9 TNFAIP1 7126
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 UBXN7 26043
Affinity Capture-MS Homo sapiens
11 PPARG 5468
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
12 POLR1C 9533
Two-hybrid Homo sapiens
13 ADRB2  
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
14 CDC37 11140
Two-hybrid Homo sapiens
15 HSPA1L 3305
Affinity Capture-MS Homo sapiens
16 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
FRET Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 PKP2 5318
Affinity Capture-MS Homo sapiens
18 VTA1 51534
Two-hybrid Homo sapiens
19 NUDT18  
Two-hybrid Homo sapiens
20 TUBA1A 7846
Affinity Capture-MS Homo sapiens
21 PSMA1 5682
Two-hybrid Homo sapiens
22 PCNA 5111
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
23 ARMC7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
24 KAT7  
Two-hybrid Homo sapiens
25 TUBB2B 347733
Affinity Capture-MS Homo sapiens
26 PFDN2 5202
Affinity Capture-MS Homo sapiens
27 HSPB8 26353
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 FLNC 2318
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 LSM3 27258
Affinity Capture-MS Homo sapiens
30 WDYHV1  
Two-hybrid Homo sapiens
31 MTURN  
Affinity Capture-MS Homo sapiens
32 GORASP2 26003
Two-hybrid Homo sapiens
33 CIT 11113
Affinity Capture-MS Homo sapiens
34 GRIN1 2902
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
35 NDST3  
Affinity Capture-MS Homo sapiens
36 ARIH1 25820
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 GRIN2A  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
38 BTBD9  
Affinity Capture-MS Homo sapiens
39 RHOA 387
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
40 POLD2 5425
Reconstituted Complex Homo sapiens
41 LNX1  
Two-hybrid Homo sapiens
42 GRIN2B  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
43 C10orf2 56652
Affinity Capture-MS Homo sapiens
44 FAF1 11124
Affinity Capture-MS Homo sapiens
45 KCTD13 253980
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
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