Gene description for KIF21A
Gene name kinesin family member 21A
Gene symbol KIF21A
Other names/aliases CFEOM1
FEOM1
FEOM3A
Species Homo sapiens
 Database cross references - KIF21A
ExoCarta ExoCarta_55605
Vesiclepedia VP_55605
Entrez Gene 55605
HGNC 19349
MIM 608283
UniProt Q7Z4S6  
 KIF21A identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for KIF21A
Molecular Function
    microtubule motor activity GO:0003777 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IBA
    plus-end-directed microtubule motor activity GO:0008574 IDA
    ATP hydrolysis activity GO:0016887 IBA
    ankyrin repeat binding GO:0071532 IPI
Biological Process
    microtubule-based movement GO:0007018 IBA
    anterograde axonal transport GO:0008089 IEA
    regulation of microtubule polymerization GO:0031113 IMP
    regulation of microtubule depolymerization GO:0031114 IMP
    cortical microtubule organization GO:0043622 IMP
    regulation of axon guidance GO:1902667 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    kinesin complex GO:0005871 IBA
    microtubule GO:0005874 IBA
    cell cortex GO:0005938 IEA
    dendrite GO:0030425 IEA
    axonal growth cone GO:0044295 IDA
    presynapse GO:0098793 IEA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified KIF21A in exosomes
1
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 193
MISEV standards
EM
Biophysical techniques
CD63|CD9
Enriched markers
PHB
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 194
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 195
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KIF21A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD99L2 83692
Affinity Capture-MS Homo sapiens
2 TBX3 6926
Affinity Capture-MS Homo sapiens
3 ZNF668  
Affinity Capture-MS Homo sapiens
4 GTF2F1 2962
Co-fractionation Homo sapiens
5 KIF21B 23046
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
6 ZBTB2 57621
Affinity Capture-MS Homo sapiens
7 CCT8 10694
Affinity Capture-MS Homo sapiens
8 RNF123 63891
Affinity Capture-MS Homo sapiens
9 CCT3 7203
Affinity Capture-MS Homo sapiens
10 CCT7 10574
Affinity Capture-MS Homo sapiens
11 HADHA 3030
Co-fractionation Homo sapiens
12 CCT5 22948
Affinity Capture-MS Homo sapiens
13 CCT2 10576
Affinity Capture-MS Homo sapiens
14 TACC2 10579
Affinity Capture-MS Homo sapiens
15 CCT6A 908
Affinity Capture-MS Homo sapiens
16 PHRF1  
Affinity Capture-MS Homo sapiens
17 DGKH 160851
Affinity Capture-MS Homo sapiens
18 KANK1  
Affinity Capture-MS Homo sapiens
19 NOLC1 9221
Affinity Capture-MS Homo sapiens
20 TCP1 6950
Affinity Capture-MS Homo sapiens
21 PFDN1 5201
Affinity Capture-MS Homo sapiens
22 RPS19 6223
Co-fractionation Homo sapiens
23 ENDOD1 23052
Affinity Capture-MS Homo sapiens
24 KANK2 25959
Affinity Capture-MS Homo sapiens
25 NCOA4 8031
Affinity Capture-MS Homo sapiens
26 MSI2 124540
Affinity Capture-MS Homo sapiens
27 CCT4 10575
Affinity Capture-MS Homo sapiens
28 SRP72 6731
Co-fractionation Homo sapiens
29 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
30 SHMT2 6472
Affinity Capture-RNA Homo sapiens
31 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
32 Cct2 12461
Affinity Capture-MS Mus musculus
33 NXF1 10482
Affinity Capture-RNA Homo sapiens
34 GSK3A 2931
Affinity Capture-MS Homo sapiens
35 NOL8  
Affinity Capture-MS Homo sapiens
36 KPNA4 3840
Co-fractionation Homo sapiens
37 MAPRE3  
Proximity Label-MS Homo sapiens
38 RBM4 5936
Affinity Capture-MS Homo sapiens
39 PFDN2 5202
Affinity Capture-MS Homo sapiens
40 MOV10 4343
Affinity Capture-RNA Homo sapiens
41 ZNF106  
Affinity Capture-MS Homo sapiens
42 IMPDH2 3615
Proximity Label-MS Homo sapiens
43 GTF2F2 2963
Co-fractionation Homo sapiens
44 NTRK1 4914
Affinity Capture-MS Homo sapiens
45 VARS 7407
Co-fractionation Homo sapiens
46 MAP3K4 4216
Affinity Capture-MS Homo sapiens
47 RAB11FIP2  
Affinity Capture-MS Homo sapiens
48 FIP1L1 81608
Affinity Capture-MS Homo sapiens
49 PPFIBP1 8496
Affinity Capture-MS Homo sapiens
50 NCS1 23413
Co-fractionation Homo sapiens
51 TTC9C 283237
Affinity Capture-MS Homo sapiens
52 SNW1 22938
Affinity Capture-MS Homo sapiens
53 UBL4A 8266
Affinity Capture-MS Homo sapiens
54 Kif21b  
Affinity Capture-MS Mus musculus
55 P4HA3 283208
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 PLXNA3 55558
Affinity Capture-MS Homo sapiens
57 C6orf136  
Affinity Capture-MS Homo sapiens
58 NVL  
Affinity Capture-MS Homo sapiens
59 RAD51  
Affinity Capture-MS Homo sapiens
60 DYNLL1 8655
Affinity Capture-MS Homo sapiens
61 CDC5L 988
Affinity Capture-MS Homo sapiens
62 PSMD3 5709
Cross-Linking-MS (XL-MS) Homo sapiens
63 SRP68 6730
Co-fractionation Homo sapiens
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