Gene description for DGKH
Gene name diacylglycerol kinase, eta
Gene symbol DGKH
Other names/aliases DGKeta
Species Homo sapiens
 Database cross references - DGKH
ExoCarta ExoCarta_160851
Entrez Gene 160851
HGNC 2854
MIM 604071
UniProt Q86XP1  
 DGKH identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for DGKH
Molecular Function
    metal ion binding GO:0046872 IEA
    diacylglycerol kinase activity GO:0004143 IMP
    ATP binding GO:0005524 IEA
Biological Process
    protein oligomerization GO:0051259 IMP
    intracellular signal transduction GO:0035556 IEA
    blood coagulation GO:0007596 TAS
    phosphorylation GO:0016310 IEA
    platelet activation GO:0030168 TAS
    protein kinase C-activating G-protein coupled receptor signaling pathway GO:0007205 IMP
Subcellular Localization
    plasma membrane GO:0005886 TAS
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    endosome GO:0005768 IDA
    nuclear membrane GO:0031965 IDA
 Experiment description of studies that identified DGKH in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for DGKH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DGKD 8527
Affinity Capture-Western Homo sapiens
2 DGKH 160851
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which DGKH is involved
PathwayEvidenceSource
Effects of PIP2 hydrolysis TAS Reactome





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