Gene description for DGKD
Gene name diacylglycerol kinase, delta 130kDa
Gene symbol DGKD
Other names/aliases DGKdelta
dgkd-2
Species Homo sapiens
 Database cross references - DGKD
ExoCarta ExoCarta_8527
Entrez Gene 8527
HGNC 2851
MIM 601826
UniProt Q16760  
 DGKD identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for DGKD
Molecular Function
    diacylglycerol kinase activity GO:0004143 TAS
    metal ion binding GO:0046872 IEA
    ATP binding GO:0005524 IEA
    diacylglycerol binding GO:0019992 NAS
    protein binding GO:0005515 IPI
    protein heterodimerization activity GO:0046982 IDA
    protein homodimerization activity GO:0042803 IDA
Biological Process
    protein homooligomerization GO:0051260 IDA
    endocytosis GO:0006897 IEA
    platelet activation GO:0030168 TAS
    protein transport GO:0015031 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 NAS
    diacylglycerol metabolic process GO:0046339 NAS
    protein kinase C-activating G-protein coupled receptor signaling pathway GO:0007205 NAS
    blood coagulation GO:0007596 TAS
    multicellular organismal development GO:0007275 NAS
    response to organic substance GO:0010033 IDA
    second-messenger-mediated signaling GO:0019932 NAS
    phosphorylation GO:0016310 IEA
    signal transduction GO:0007165 TAS
    cell growth GO:0016049 NAS
Subcellular Localization
    cytoplasmic membrane-bounded vesicle GO:0016023 IDA
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified DGKD in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for DGKD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MGC15730  
Two-hybrid Homo sapiens
2 DGKD 8527
Invivo Homo sapiens
Invivo Homo sapiens
3 HAX1  
Two-hybrid Homo sapiens
4 DGKH 160851
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which DGKD is involved
PathwayEvidenceSource
Effects of PIP2 hydrolysis TAS Reactome





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