Gene description for PLXNA3
Gene name plexin A3
Gene symbol PLXNA3
Other names/aliases 6.3
HSSEXGENE
PLXN3
PLXN4
XAP-6
Species Homo sapiens
 Database cross references - PLXNA3
ExoCarta ExoCarta_55558
Vesiclepedia VP_55558
Entrez Gene 55558
HGNC 9101
MIM 300022
UniProt P51805  
 PLXNA3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for PLXNA3
Molecular Function
    protein binding GO:0005515 IPI
    semaphorin receptor activity GO:0017154 IBA
    semaphorin receptor activity GO:0017154 ISS
Biological Process
    axon guidance GO:0007411 ISS
    synapse assembly GO:0007416 IBA
    motor neuron axon guidance GO:0008045 IBA
    facial nerve structural organization GO:0021612 ISS
    olfactory nerve formation GO:0021628 IEA
    trigeminal nerve structural organization GO:0021637 ISS
    hippocampus development GO:0021766 ISS
    branchiomotor neuron axon guidance GO:0021785 ISS
    gonadotrophin-releasing hormone neuronal migration to the hypothalamus GO:0021828 IEA
    pyramidal neuron development GO:0021860 ISS
    regulation of cell migration GO:0030334 IBA
    negative regulation of axon extension involved in axon guidance GO:0048843 IEA
    axon extension involved in axon guidance GO:0048846 IEA
    negative chemotaxis GO:0050919 IEA
    positive regulation of cytoskeleton organization GO:0051495 ISS
    semaphorin-plexin signaling pathway GO:0071526 IBA
    semaphorin-plexin signaling pathway GO:0071526 ISS
    semaphorin-plexin signaling pathway GO:0071526 NAS
    neuron projection guidance GO:0097485 ISS
Subcellular Localization
    semaphorin receptor complex GO:0002116 IBA
    semaphorin receptor complex GO:0002116 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified PLXNA3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
10
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PLXNA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FMOD 2331
Affinity Capture-MS Homo sapiens
2 Cyr61  
Affinity Capture-MS Mus musculus
3 CEACAM8 1088
Affinity Capture-MS Homo sapiens
4 TK1 7083
Two-hybrid Homo sapiens
5 HLA-E 3133
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 Iqgap3 404710
Affinity Capture-MS Mus musculus
7 MKNK2 2872
Affinity Capture-MS Homo sapiens
8 GAK 2580
Affinity Capture-MS Homo sapiens
9 MFAP4 4239
Affinity Capture-MS Homo sapiens
10 Ndc80  
Affinity Capture-MS Mus musculus
11 TRIM55  
Two-hybrid Homo sapiens
12 SLURP1  
Affinity Capture-MS Homo sapiens
13 HSF2  
Affinity Capture-MS Homo sapiens
14 Med22  
Affinity Capture-MS Mus musculus
15 EXOSC2 23404
Affinity Capture-MS Homo sapiens
16 NOLC1 9221
Cross-Linking-MS (XL-MS) Homo sapiens
17 CLEC2B  
Affinity Capture-MS Homo sapiens
18 KLRC1  
Affinity Capture-MS Homo sapiens
19 CST9L  
Affinity Capture-MS Homo sapiens
20 ST8SIA4 7903
Affinity Capture-MS Homo sapiens
21 Gspt1 14852
Affinity Capture-MS Mus musculus
22 BTNL2  
Affinity Capture-MS Homo sapiens
23 TMEM106A  
Affinity Capture-MS Homo sapiens
24 ISLR 3671
Affinity Capture-MS Homo sapiens
25 CSNK2B 1460
Two-hybrid Homo sapiens
26 PARD6A  
Affinity Capture-MS Homo sapiens
27 KIF21A 55605
Affinity Capture-MS Homo sapiens
28 HLA-G 3135
Affinity Capture-MS Homo sapiens
29 Tuba3a 22144
Affinity Capture-MS Mus musculus
30 MAPK8IP2  
Two-hybrid Homo sapiens
31 Mad2l1bp  
Affinity Capture-MS Mus musculus
32 TAZ  
Affinity Capture-MS Homo sapiens
33 SPX  
Affinity Capture-MS Homo sapiens
34 SMC6  
Affinity Capture-MS Homo sapiens
35 KLC4 89953
Affinity Capture-MS Homo sapiens
36 PSMD14 10213
Affinity Capture-MS Homo sapiens
37 BAG6 7917
Affinity Capture-MS Homo sapiens
38 IDS  
Affinity Capture-MS Homo sapiens
39 PPP2CB 5516
Two-hybrid Homo sapiens
40 SDF2L1 23753
Affinity Capture-MS Homo sapiens
41 FIBIN  
Affinity Capture-MS Homo sapiens
42 CRISP2  
Affinity Capture-MS Homo sapiens
43 C1orf54  
Affinity Capture-MS Homo sapiens
44 SIRPD  
Affinity Capture-MS Homo sapiens
45 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
46 CCDC22 28952
Affinity Capture-MS Homo sapiens
47 LYPD4  
Affinity Capture-MS Homo sapiens
48 TRIM63  
Two-hybrid Homo sapiens
49 LY86  
Affinity Capture-MS Homo sapiens
50 BAG3 9531
Affinity Capture-MS Homo sapiens
51 GPIHBP1  
Affinity Capture-MS Homo sapiens
52 SPATS2L 26010
Affinity Capture-MS Homo sapiens
53 GGH 8836
Affinity Capture-MS Homo sapiens
54 PCDHB15  
Affinity Capture-MS Homo sapiens
55 POLK  
Affinity Capture-MS Homo sapiens
56 CBLN4  
Affinity Capture-MS Homo sapiens
57 LYZL1  
Affinity Capture-MS Homo sapiens
58 IL5RA  
Affinity Capture-MS Homo sapiens
59 Ect2  
Affinity Capture-MS Mus musculus
60 EPHA7 2045
Affinity Capture-MS Homo sapiens
61 SGTB  
Affinity Capture-MS Homo sapiens
62 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
63 ACTR5 79913
Affinity Capture-MS Homo sapiens
64 Tpx2  
Affinity Capture-MS Mus musculus
65 CNTROB  
Affinity Capture-MS Homo sapiens
66 FBXO2 26232
Affinity Capture-MS Homo sapiens
67 SCGB2A2  
Affinity Capture-MS Homo sapiens
68 HYOU1 10525
Affinity Capture-MS Homo sapiens
69 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
70 Kctd5  
Affinity Capture-MS Mus musculus
71 Nedd1  
Affinity Capture-MS Mus musculus
72 Fign  
Affinity Capture-MS Mus musculus
73 EDDM3B  
Affinity Capture-MS Homo sapiens
74 NRG1 3084
Affinity Capture-MS Homo sapiens
75 GALNT10 55568
Affinity Capture-MS Homo sapiens
76 CA6 765
Affinity Capture-MS Homo sapiens
77 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
78 SMN1 6606
Two-hybrid Homo sapiens
79 SCGB1D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 IGLL5 100423062
Affinity Capture-MS Homo sapiens
81 ASIC4  
Affinity Capture-MS Homo sapiens
82 Kif1c  
Affinity Capture-MS Mus musculus
83 MLH1 4292
Affinity Capture-MS Homo sapiens
84 APOM 55937
Affinity Capture-MS Homo sapiens
85 SFTPC  
Affinity Capture-MS Homo sapiens
86 EDDM3A  
Affinity Capture-MS Homo sapiens
87 CLEC12B  
Affinity Capture-MS Homo sapiens
88 C1QB 713
Affinity Capture-MS Homo sapiens
89 BRICD5  
Affinity Capture-MS Homo sapiens
90 Kif4  
Affinity Capture-MS Mus musculus
91 DEFB135  
Affinity Capture-MS Homo sapiens
92 PTPRK 5796
Affinity Capture-MS Homo sapiens
93 OAZ1 4946
Affinity Capture-MS Homo sapiens
94 Trappc11  
Affinity Capture-MS Mus musculus
95 USF1  
Affinity Capture-MS Homo sapiens
96 PTCH1  
Affinity Capture-MS Homo sapiens
97 PCDHB3  
Affinity Capture-MS Homo sapiens
98 KLK3  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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