Experiment description of studies that identified MKNK2 in exosomes

1 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

2 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

Protein-protein interactions for MKNK2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 CD99L2 83692 Affinity Capture-MS Homo sapiens 2 WDR46   Affinity Capture-MS Homo sapiens 3 CELF1 10658 Affinity Capture-MS Homo sapiens 4 NUDT21 11051 Affinity Capture-MS Homo sapiens 5 ESR2   Two-hybrid Homo sapiens 6 KHSRP 8570 Affinity Capture-MS Homo sapiens 7 EXOC3 11336 Affinity Capture-MS Homo sapiens 8 TRAP1 10131 Affinity Capture-MS Homo sapiens 9 USP15 9958 Affinity Capture-MS Homo sapiens 10 EIF4G1 1981 Affinity Capture-Western Homo sapiens Biochemical Activity Homo sapiens 11 MAPK1 5594 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens 12 SYMPK 8189 Affinity Capture-MS Homo sapiens 13 BHLHE41   Affinity Capture-MS Homo sapiens 14 RICTOR 253260 Affinity Capture-MS Homo sapiens 15 RBX1 9978 Two-hybrid Homo sapiens 16 ELAVL1 1994 Affinity Capture-RNA Homo sapiens 17 VHL   Affinity Capture-Western Homo sapiens Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 18 CSTF2 1478 Affinity Capture-MS Homo sapiens 19 VBP1 7411 Two-hybrid Homo sapiens 20 ZMYM1 79830 Affinity Capture-MS Homo sapiens 21 BAG6 7917 Affinity Capture-MS Homo sapiens 22 MIF 4282 Affinity Capture-MS Homo sapiens 23 WWP2 11060 Affinity Capture-MS Homo sapiens 24 RBM4 5936 Affinity Capture-MS Homo sapiens 25 RBM26 64062 Affinity Capture-MS Homo sapiens 26 GAPDH 2597 Affinity Capture-MS Homo sapiens 27 VCL 7414 Affinity Capture-MS Homo sapiens 28 ZCCHC6 79670 Affinity Capture-MS Homo sapiens 29 NOLC1 9221 Affinity Capture-MS Homo sapiens 30 RPL7A 6130 Two-hybrid Homo sapiens 31 RAB11FIP2   Affinity Capture-MS Homo sapiens 32 MAPK14 1432 Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Co-localization Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens 33 PLXNA3 55558 Affinity Capture-MS Homo sapiens 34 CUL2 8453 Two-hybrid Homo sapiens 35 DDX58 23586 Affinity Capture-RNA Homo sapiens 36 MAPK8 5599 Two-hybrid Homo sapiens 37 MRRF   Affinity Capture-MS Homo sapiens 38 HAUS7   Affinity Capture-MS Homo sapiens 39 ADCK1   Affinity Capture-MS Homo sapiens 40 TICAM1   Affinity Capture-MS Homo sapiens View the network

Pathways in which MKNK2 is involved

No pathways found