Gene description for TBX3
Gene name T-box 3
Gene symbol TBX3
Other names/aliases TBX3-ISO
UMS
XHL
Species Homo sapiens
 Database cross references - TBX3
ExoCarta ExoCarta_6926
Vesiclepedia VP_6926
Entrez Gene 6926
HGNC 11602
MIM 601621
UniProt O15119  
 TBX3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for TBX3
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 IDA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    protein binding GO:0005515 IPI
    sequence-specific DNA binding GO:0043565 IDA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 ISS
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    skeletal system development GO:0001501 IMP
    blood vessel development GO:0001568 IEA
    in utero embryonic development GO:0001701 IEA
    cell fate specification GO:0001708 IBA
    heart looping GO:0001947 IEA
    outflow tract morphogenesis GO:0003151 IEA
    atrioventricular bundle cell differentiation GO:0003167 IEA
    endocardial cushion formation GO:0003272 IMP
    DNA-templated transcription GO:0006351 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    regulation of transcription by RNA polymerase II GO:0006357 TAS
    positive regulation of cell population proliferation GO:0008284 IDA
    anterior/posterior axis specification, embryo GO:0008595 IMP
    animal organ morphogenesis GO:0009887 IDA
    specification of animal organ position GO:0010159 IEA
    stem cell population maintenance GO:0019827 IEA
    limbic system development GO:0021761 IEA
    male genitalia development GO:0030539 IMP
    female genitalia development GO:0030540 IMP
    negative regulation of epithelial cell differentiation GO:0030857 IEA
    mammary gland development GO:0030879 IMP
    regulation of protein stability GO:0031647 IEA
    luteinizing hormone secretion GO:0032275 IMP
    embryonic forelimb morphogenesis GO:0035115 IMP
    embryonic hindlimb morphogenesis GO:0035116 IEA
    forelimb morphogenesis GO:0035136 IDA
    atrioventricular canal development GO:0036302 ISS
    embryonic digit morphogenesis GO:0042733 IMP
    negative regulation of apoptotic process GO:0043066 IDA
    negative regulation of myoblast differentiation GO:0045662 IDA
    positive regulation of cell cycle GO:0045787 IDA
    negative regulation of DNA-templated transcription GO:0045892 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    follicle-stimulating hormone secretion GO:0046884 IMP
    mesoderm morphogenesis GO:0048332 IMP
    semicircular canal morphogenesis GO:0048752 IEA
    smooth muscle cell differentiation GO:0051145 IEA
    roof of mouth development GO:0060021 IEA
    cardiac epithelial to mesenchymal transition GO:0060317 IMP
    ventricular septum morphogenesis GO:0060412 IEA
    branching involved in mammary gland duct morphogenesis GO:0060444 IEA
    mammary placode formation GO:0060596 IEA
    cardiac muscle cell fate commitment GO:0060923 IEA
    sinoatrial node cell development GO:0060931 IDA
    hepatoblast differentiation GO:0061017 IEA
    regulation of protein complex stability GO:0061635 IEA
    stem cell proliferation GO:0072089 IEA
    ureteric peristalsis GO:0072105 IEA
    cellular senescence GO:0090398 IDA
    cardiac jelly development GO:1905072 IMP
    atrioventricular canal morphogenesis GO:1905222 ISS
    negative regulation of cell proliferation involved in heart morphogenesis GO:2000137 IMP
    positive regulation of stem cell proliferation GO:2000648 IEA
    negative regulation of stem cell differentiation GO:2000737 IEA
Subcellular Localization
    chromatin GO:0000785 IBA
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cilium GO:0005929 IEA
 Experiment description of studies that identified TBX3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for TBX3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDCA5  
Affinity Capture-MS Homo sapiens
2 TBX3 6926
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
3 SLC25A3 5250
Affinity Capture-MS Homo sapiens
4 AFG3L2 10939
Affinity Capture-MS Homo sapiens
5 RPS27A 6233
Affinity Capture-MS Homo sapiens
6 HDAC4  
Affinity Capture-MS Homo sapiens
7 KIF21B 23046
Affinity Capture-MS Homo sapiens
8 DNAJC11 55735
Affinity Capture-MS Homo sapiens
9 HDAC2 3066
Affinity Capture-Western Homo sapiens
10 KIF21A 55605
Affinity Capture-MS Homo sapiens
11 Naa15 74838
Affinity Capture-MS Mus musculus
12 PRR20A  
Two-hybrid Homo sapiens
13 MYL6 4637
Affinity Capture-MS Homo sapiens
14 CDK8 1024
Affinity Capture-MS Homo sapiens
15 HSPA8 3312
Affinity Capture-MS Homo sapiens
16 PDIA3 2923
Affinity Capture-MS Homo sapiens
17 RBMX 27316
Affinity Capture-MS Homo sapiens
18 AES 166
Two-hybrid Homo sapiens
19 UFSP1  
Two-hybrid Homo sapiens
20 PRR20C  
Two-hybrid Homo sapiens
21 TOLLIP 54472
Two-hybrid Homo sapiens
22 Smad3  
Affinity Capture-MS Mus musculus
23 ZNF34  
Two-hybrid Homo sapiens
24 SIRPA 140885
Reconstituted Complex Homo sapiens
25 MAPK3 5595
Affinity Capture-MS Homo sapiens
26 PLEKHF2 79666
Two-hybrid Homo sapiens
27 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
28 USP10 9100
Affinity Capture-MS Homo sapiens
29 PRR20D  
Two-hybrid Homo sapiens
30 EMD 2010
Affinity Capture-MS Homo sapiens
31 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
32 CALM1 801
Affinity Capture-MS Homo sapiens
33 HDAC1 3065
Affinity Capture-Western Homo sapiens
34 EIF4A3 9775
Affinity Capture-MS Homo sapiens
35 HSPA1A 3303
Affinity Capture-MS Homo sapiens
36 CSF3 1440
Two-hybrid Homo sapiens
37 SVIL 6840
Affinity Capture-MS Homo sapiens
38 HNRNPK 3190
Affinity Capture-MS Homo sapiens
39 PABPC4 8761
Affinity Capture-MS Homo sapiens
40 TMPO 7112
Affinity Capture-MS Homo sapiens
41 PRR20B  
Two-hybrid Homo sapiens
42 HDAC3 8841
Affinity Capture-Western Homo sapiens
43 MKRN2 23609
Affinity Capture-RNA Homo sapiens
44 PRR20E  
Two-hybrid Homo sapiens
45 HNRNPLL 92906
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 CA8  
Two-hybrid Homo sapiens
47 DCP1A 55802
Affinity Capture-MS Homo sapiens
48 CTNNB1 1499
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 XRCC6 2547
Affinity Capture-MS Homo sapiens
50 PML 5371
Affinity Capture-Western Homo sapiens
51 DHX9 1660
Affinity Capture-MS Homo sapiens
52 NUFIP2 57532
Affinity Capture-MS Homo sapiens
53 GUSBP5  
Affinity Capture-MS Homo sapiens
54 EWSR1 2130
Affinity Capture-MS Homo sapiens
55 LMNA 4000
Affinity Capture-MS Homo sapiens
56 HDAC5 10014
Affinity Capture-Western Homo sapiens
57 PFDN5 5204
Two-hybrid Homo sapiens
58 ATP5B 506
Affinity Capture-MS Homo sapiens
59 XRCC5 7520
Affinity Capture-MS Homo sapiens
60 SP100 6672
Affinity Capture-MS Homo sapiens
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