Gene description for RASA1
Gene name RAS p21 protein activator (GTPase activating protein) 1
Gene symbol RASA1
Other names/aliases CM-AVM
CMAVM
GAP
PKWS
RASA
RASGAP
p120GAP
p120RASGAP
Species Homo sapiens
 Database cross references - RASA1
ExoCarta ExoCarta_5921
Vesiclepedia VP_5921
Entrez Gene 5921
HGNC 9871
MIM 139150
UniProt P20936  
 RASA1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for RASA1
Molecular Function
    phosphotyrosine residue binding GO:0001784 IPI
    GTPase activity GO:0003924 TAS
    GTPase activator activity GO:0005096 EXP
    signaling receptor binding GO:0005102 IPI
    protein binding GO:0005515 IPI
    potassium channel inhibitor activity GO:0019870 NAS
    GTPase binding GO:0051020 IPI
Biological Process
    mitotic cytokinesis GO:0000281 ISS
    vasculogenesis GO:0001570 ISS
    negative regulation of cell-matrix adhesion GO:0001953 IDA
    negative regulation of cell adhesion GO:0007162 IDA
    signal transduction GO:0007165 IDA
    regulation of cell shape GO:0008360 NAS
    regulation of actin filament polymerization GO:0030833 IDA
    intracellular signal transduction GO:0035556 NAS
    negative regulation of apoptotic process GO:0043066 IDA
    negative regulation of neuron apoptotic process GO:0043524 ISS
    ephrin receptor signaling pathway GO:0048013 TAS
    blood vessel morphogenesis GO:0048514 IMP
    regulation of RNA metabolic process GO:0051252 NAS
Subcellular Localization
    ruffle GO:0001726 IEA
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified RASA1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
7
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RASA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 Mbp 17196
Protein-peptide Mus musculus
3 DOK2 9046
Affinity Capture-Western Homo sapiens
4 SOCS3 9021
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
5 NTRK1 4914
Affinity Capture-MS Homo sapiens
6 SPSB1  
Affinity Capture-Western Homo sapiens
7 NCK1 4690
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
8 GRB2 2885
Affinity Capture-Western Homo sapiens
9 HRAS 3265
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 YES1 7525
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
11 CSK 1445
Reconstituted Complex Homo sapiens
12 KDR  
Affinity Capture-Western Homo sapiens
13 RHOC 389
Co-fractionation Homo sapiens
14 ZNF579  
Two-hybrid Homo sapiens
15 SERPINA4 5267
Two-hybrid Homo sapiens
16 CAV2 858
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 EPHB2 2048
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
18 SLC9A2  
Reconstituted Complex Homo sapiens
19 KIT 3815
Reconstituted Complex Homo sapiens
20 LCK 3932
Biochemical Activity Homo sapiens
21 INSR 3643
Affinity Capture-Western Homo sapiens
22 DNAJA3 9093
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
23 ERBB4 2066
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
24 SOCS1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 MAP4K4 9448
Affinity Capture-Western Homo sapiens
26 EGFR 1956
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
27 Dok1  
Affinity Capture-Western Mus musculus
28 HCK 3055
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
29 IGF1R 3480
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
30 ERBB3 2065
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
31 ANKRD36B  
Affinity Capture-MS Homo sapiens
32 RAB12 201475
Co-fractionation Homo sapiens
33 APP 351
Reconstituted Complex Homo sapiens
34 GAB1  
Reconstituted Complex Homo sapiens
35 BTRC 8945
Phenotypic Suppression Homo sapiens
36 HTT 3064
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
37 MET 4233
Reconstituted Complex Homo sapiens
38 DOK1 1796
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
39 EZH2  
Two-hybrid Homo sapiens
40 CD5 921
Affinity Capture-Western Homo sapiens
41 BBS10  
Two-hybrid Homo sapiens
42 ANXA6 309
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
43 G3BP1 10146
Affinity Capture-Western Homo sapiens
44 EIF1 10209
Two-hybrid Homo sapiens
45 Khdrbs1 20218
Reconstituted Complex Mus musculus
46 PDGFRB 5159
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
47 PXN 5829
Affinity Capture-Western Homo sapiens
48 RNPEP 6051
Co-fractionation Homo sapiens
49 PTK2B 2185
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
50 ZAP70 7535
Affinity Capture-Western Homo sapiens
51 ERBB2 2064
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
52 XRCC6 2547
Two-hybrid Homo sapiens
53 FBXL6  
Proximity Label-MS Homo sapiens
54 NFKBIA  
Phenotypic Suppression Homo sapiens
55 SRC 6714
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
56 GNB2L1 10399
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
57 KHDRBS1 10657
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
58 ARHGAP35 2909
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
59 TRMT2A 27037
Two-hybrid Homo sapiens
60 PLCG1 5335
Affinity Capture-Western Homo sapiens
61 EPHB3 2049
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
62 AR 367
Reconstituted Complex Homo sapiens
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