Gene description for EPHB3
Gene name EPH receptor B3
Gene symbol EPHB3
Other names/aliases ETK2
HEK2
TYRO6
Species Homo sapiens
 Database cross references - EPHB3
ExoCarta ExoCarta_2049
Entrez Gene 2049
HGNC 3394
MIM 601839
UniProt P54753  
 EPHB3 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 19837982    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for EPHB3
Molecular Function
    ephrin receptor activity GO:0005003 IDA
    ATP binding GO:0005524 IEA
    axon guidance receptor activity GO:0008046 IEA
Biological Process
    corpus callosum development GO:0022038 ISS
    positive regulation of synapse assembly GO:0051965 ISS
    angiogenesis GO:0001525 ISS
    urogenital system development GO:0001655 ISS
    regulation of axonogenesis GO:0050770 ISS
    cell migration GO:0016477 IDA
    peptidyl-tyrosine phosphorylation GO:0018108 IEA
    axon guidance GO:0007411 TAS
    retinal ganglion cell axon guidance GO:0031290 IEA
    axonal fasciculation GO:0007413 ISS
    dendritic spine development GO:0060996 ISS
    thymus development GO:0048538 ISS
    central nervous system projection neuron axonogenesis GO:0021952 IEA
    palate development GO:0060021 ISS
    protein autophosphorylation GO:0046777 IDA
    regulation of cell-cell adhesion GO:0022407 IDA
    ephrin receptor signaling pathway GO:0048013 TAS
    regulation of GTPase activity GO:0043087 IDA
    dendritic spine morphogenesis GO:0060997 ISS
    digestive tract morphogenesis GO:0048546 ISS
    substrate adhesion-dependent cell spreading GO:0034446 IDA
Subcellular Localization
    cytosol GO:0005829 TAS
    dendrite GO:0030425 IEA
    plasma membrane GO:0005886 TAS
    integral component of plasma membrane GO:0005887 IDA
    extracellular region GO:0005576 TAS
 Experiment description of studies that identified EPHB3 in exosomes
1
Experiment ID 21
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EPHB3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RASA1 5921
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 CRK 1398
Reconstituted Complex Homo sapiens
3 EFNB3  
Reconstituted Complex Homo sapiens
4 RYK  
Affinity Capture-Western Homo sapiens
5 MLLT4 4301
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
6 FYN 2534
Reconstituted Complex Homo sapiens
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