Gene description for EFEMP2
Gene name EGF containing fibulin-like extracellular matrix protein 2
Gene symbol EFEMP2
Other names/aliases ARCL1B
FBLN4
MBP1
UPH1
Species Homo sapiens
 Database cross references - EFEMP2
ExoCarta ExoCarta_30008
Vesiclepedia VP_30008
Entrez Gene 30008
HGNC 3219
MIM 604633
UniProt O95967  
 EFEMP2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Saliva 19199708    
 Gene ontology annotations for EFEMP2
Molecular Function
    extracellular matrix structural constituent GO:0005201 RCA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    heparin binding GO:0008201 IDA
    protein homodimerization activity GO:0042803 IDA
Biological Process
    aorta development GO:0035904 ISS
    elastic fiber assembly GO:0048251 IMP
    elastic fiber assembly GO:0048251 ISS
    aorta smooth muscle tissue morphogenesis GO:0060414 ISS
    vascular associated smooth muscle cell development GO:0097084 ISS
    regulation of collagen fibril organization GO:1904026 ISS
    positive regulation of collagen fibril organization GO:1904028 ISS
    negative regulation of vascular associated smooth muscle cell proliferation GO:1904706 ISS
    positive regulation of aortic smooth muscle cell differentiation GO:1904831 ISS
    positive regulation of smooth muscle cell-matrix adhesion GO:1905609 IMP
Subcellular Localization
    microfibril GO:0001527 ISS
    extracellular region GO:0005576 TAS
    basement membrane GO:0005604 ISS
    extracellular matrix GO:0031012 IDA
    extracellular matrix GO:0031012 ISS
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 HDA
    elastic fiber GO:0071953 IDA
    elastic fiber GO:0071953 ISS
    extracellular vesicle GO:1903561 HDA
 Experiment description of studies that identified EFEMP2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for EFEMP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF699  
Two-hybrid Homo sapiens
2 HDAC4  
Two-hybrid Homo sapiens
3 KRTAP11-1  
Two-hybrid Homo sapiens
4 RBPMS 11030
Two-hybrid Homo sapiens
5 SPATA24  
Two-hybrid Homo sapiens
6 AES 166
Two-hybrid Homo sapiens
7 PLSCR1 5359
Two-hybrid Homo sapiens
8 HSPA12B 116835
Two-hybrid Homo sapiens
9 MDFI  
Two-hybrid Homo sapiens
10 SLC25A48  
Two-hybrid Homo sapiens
11 IL16  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 RHOXF2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
13 ATN1  
Two-hybrid Homo sapiens
14 ZNF426  
Two-hybrid Homo sapiens
15 CCNK  
Two-hybrid Homo sapiens
16 NTM 50863
Two-hybrid Homo sapiens
17 LCE3C  
Two-hybrid Homo sapiens
18 FTO 79068
Two-hybrid Homo sapiens
19 C3 718
Two-hybrid Homo sapiens
20 ZNF638 27332
Two-hybrid Homo sapiens
21 COL8A1 1295
Two-hybrid Homo sapiens
22 MRPL12 6182
Two-hybrid Homo sapiens
23 LCE2D  
Two-hybrid Homo sapiens
24 UBQLN1 29979
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
25 ANAPC11  
Two-hybrid Homo sapiens
26 CATSPER1  
Two-hybrid Homo sapiens
27 KRTAP19-5  
Two-hybrid Homo sapiens
28 UBQLN4 56893
Two-hybrid Homo sapiens
29 FBXW5  
Two-hybrid Homo sapiens
30 GFI1B  
Two-hybrid Homo sapiens
31 LCE1E  
Two-hybrid Homo sapiens
32 LCE3A  
Two-hybrid Homo sapiens
33 ADAMTSL4  
Two-hybrid Homo sapiens
34 HSPBP1 23640
Two-hybrid Homo sapiens
35 LCE2C  
Two-hybrid Homo sapiens
36 AQP1 358
Two-hybrid Homo sapiens
37 AMMECR1  
Two-hybrid Homo sapiens
38 TLX3  
Two-hybrid Homo sapiens
39 USP21  
Two-hybrid Homo sapiens
40 FAM110A  
Two-hybrid Homo sapiens
41 LINGO1 84894
Two-hybrid Homo sapiens
42 CCDC33 80125
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
43 TRIM42  
Two-hybrid Homo sapiens
44 BAG6 7917
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
45 CNNM3 26505
Two-hybrid Homo sapiens
46 FAM107A  
Two-hybrid Homo sapiens
47 LCE3D  
Two-hybrid Homo sapiens
48 SPRY4 81848
Two-hybrid Homo sapiens
49 SPATA3  
Two-hybrid Homo sapiens
50 ZNF837  
Two-hybrid Homo sapiens
51 LCE3E  
Two-hybrid Homo sapiens
52 RBP3  
Two-hybrid Homo sapiens
53 MEOX2  
Two-hybrid Homo sapiens
54 ARRDC3 57561
Two-hybrid Homo sapiens
55 ZNF581  
Two-hybrid Homo sapiens
56 ZNF345  
Two-hybrid Homo sapiens
57 FAM124B  
Two-hybrid Homo sapiens
58 PRPF31 26121
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
59 PLSCR4  
Two-hybrid Homo sapiens
60 ATXN1 6310
Two-hybrid Homo sapiens
61 CACNA1A  
Two-hybrid Homo sapiens
62 RAB40B  
Two-hybrid Homo sapiens
63 RERE 473
Two-hybrid Homo sapiens
64 CXCL5  
Two-hybrid Homo sapiens
65 CDPF1  
Two-hybrid Homo sapiens
66 HPCAL1 3241
Two-hybrid Homo sapiens
67 NOS3  
Two-hybrid Homo sapiens
68 KRTAP10-8  
Two-hybrid Homo sapiens
69 DOK7 285489
Two-hybrid Homo sapiens
70 KLF1  
Two-hybrid Homo sapiens
71 C9orf9  
Two-hybrid Homo sapiens
72 RNF138  
Two-hybrid Homo sapiens
73 FAM90A1  
Two-hybrid Homo sapiens
74 HOXA1 3198
Two-hybrid Homo sapiens
75 SPATA8  
Two-hybrid Homo sapiens
76 OTX1  
Two-hybrid Homo sapiens
77 ZNF670  
Two-hybrid Homo sapiens
78 Hoxa1  
Two-hybrid Mus musculus
PCA Mus musculus
Affinity Capture-Western Mus musculus
79 SGTB  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
80 ZNF558  
Two-hybrid Homo sapiens
81 HHIPL1 84439
Two-hybrid Homo sapiens
82 FAM74A4  
Two-hybrid Homo sapiens
83 DGCR6  
Two-hybrid Homo sapiens
84 RHPN1  
Two-hybrid Homo sapiens
85 RIBC2  
Two-hybrid Homo sapiens
86 ZNF587  
Two-hybrid Homo sapiens
87 SGTA 6449
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
88 APEX2  
Two-hybrid Homo sapiens
89 KRTAP3-2  
Two-hybrid Homo sapiens
90 NEDD4L 23327
Biochemical Activity Homo sapiens
91 ZNF263  
Two-hybrid Homo sapiens
92 LCE1C  
Two-hybrid Homo sapiens
93 CLPP 8192
Two-hybrid Homo sapiens
94 KRTAP13-3  
Two-hybrid Homo sapiens
95 PRR32  
Two-hybrid Homo sapiens
96 STK16 8576
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
97 CYSRT1 375791
Two-hybrid Homo sapiens
98 LCE1A  
Two-hybrid Homo sapiens
99 HHEX  
Two-hybrid Homo sapiens
100 PROP1  
Two-hybrid Homo sapiens
101 TP53 7157
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
102 C5orf24 134553
Two-hybrid Homo sapiens
103 ATXN7  
Two-hybrid Homo sapiens
104 NUFIP2 57532
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
105 INCA1  
Two-hybrid Homo sapiens
106 PIDD1  
Two-hybrid Homo sapiens
107 RBAK  
Two-hybrid Homo sapiens
108 PTGER3  
Two-hybrid Homo sapiens
109 CREB5 9586
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
110 THAP7  
Two-hybrid Homo sapiens
111 LBX1  
Two-hybrid Homo sapiens
112 KRTAP26-1  
Two-hybrid Homo sapiens
113 KRTAP12-2  
Two-hybrid Homo sapiens
114 MXI1 4601
Two-hybrid Homo sapiens
115 ZNF768 79724
Two-hybrid Homo sapiens
116 TCAF1 9747
Two-hybrid Homo sapiens
117 ZNF57  
Two-hybrid Homo sapiens
118 TRIB3  
Two-hybrid Homo sapiens
119 SLC16A5  
Two-hybrid Homo sapiens
120 GLRX3 10539
Two-hybrid Homo sapiens
121 IGFBP6 3489
Two-hybrid Homo sapiens
122 SLC23A1 9963
Two-hybrid Homo sapiens
123 ZNF774  
Two-hybrid Homo sapiens
124 NR1D2  
Two-hybrid Homo sapiens
125 CCND3  
Two-hybrid Homo sapiens
View the network image/svg+xml



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