Gene description for SPRY4
Gene name sprouty homolog 4 (Drosophila)
Gene symbol SPRY4
Other names/aliases HH17
Species Homo sapiens
 Database cross references - SPRY4
ExoCarta ExoCarta_81848
Vesiclepedia VP_81848
Entrez Gene 81848
HGNC 15533
MIM 607984
UniProt Q9C004  
 SPRY4 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SPRY4
Molecular Function
    protein kinase inhibitor activity GO:0004860 IMP
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of fibroblast growth factor receptor signaling pathway GO:0040037 IBA
    negative regulation of Ras protein signal transduction GO:0046580 IBA
    animal organ development GO:0048513 IBA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IBA
    negative regulation of substrate adhesion-dependent cell spreading GO:1900025 IMP
    cellular response to leukemia inhibitory factor GO:1990830 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
    focal adhesion GO:0005925 HDA
    ruffle membrane GO:0032587 IEA
 Experiment description of studies that identified SPRY4 in exosomes
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
3
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SPRY4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GJD3  
Proximity Label-MS Homo sapiens
2 RAB35 11021
Proximity Label-MS Homo sapiens
3 AP2B1 163
Proximity Label-MS Homo sapiens
4 ANKFY1 51479
Proximity Label-MS Homo sapiens
5 NUTF2 10204
Two-hybrid Homo sapiens
6 EBAG9 9166
Proximity Label-MS Homo sapiens
7 SPRY2 10253
Affinity Capture-Western Homo sapiens
8 GRB2 2885
Affinity Capture-Western Homo sapiens
9 ASGR1 432
Affinity Capture-MS Homo sapiens
10 LAMTOR1 55004
Proximity Label-MS Homo sapiens
11 MARCKS 4082
Proximity Label-MS Homo sapiens
12 LAMP3  
Proximity Label-MS Homo sapiens
13 RAB5A 5868
Proximity Label-MS Homo sapiens
14 KRTAP5-11  
Two-hybrid Homo sapiens
15 GJA1 2697
Proximity Label-MS Homo sapiens
16 ADAM12 8038
Two-hybrid Homo sapiens
17 RAB9A 9367
Proximity Label-MS Homo sapiens
18 ZDHHC17 23390
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
19 LAMP1 3916
Proximity Label-MS Homo sapiens
20 KLHL38  
Two-hybrid Homo sapiens
21 TSPAN15 23555
Affinity Capture-MS Homo sapiens
22 LMAN1 3998
Proximity Label-MS Homo sapiens
23 LAMP2 3920
Proximity Label-MS Homo sapiens
24 ARF6 382
Proximity Label-MS Homo sapiens
25 ORMDL3 94103
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 TESK1 7016
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 CXADR 1525
Proximity Label-MS Homo sapiens
28 HOXA1 3198
Two-hybrid Homo sapiens
29 C11orf52 91894
Proximity Label-MS Homo sapiens
30 ELOVL5 60481
Proximity Label-MS Homo sapiens
31 OTX1  
Two-hybrid Homo sapiens
32 CREB5 9586
Two-hybrid Homo sapiens
33 MCAM 4162
Proximity Label-MS Homo sapiens
34 CBL 867
Affinity Capture-Western Homo sapiens
35 RAB4A 5867
Proximity Label-MS Homo sapiens
36 KCNE3  
Affinity Capture-MS Homo sapiens
37 NEIL1  
Affinity Capture-MS Homo sapiens
38 ZDHHC13 54503
Affinity Capture-MS Homo sapiens
39 B3GAT1  
Proximity Label-MS Homo sapiens
40 RAB2A 5862
Proximity Label-MS Homo sapiens
41 MYOF 26509
Affinity Capture-MS Homo sapiens
42 POU4F2  
Two-hybrid Homo sapiens
43 RAB11A 8766
Proximity Label-MS Homo sapiens
44 RAB3B 5865
Proximity Label-MS Homo sapiens
45 RAF1 5894
Affinity Capture-Western Homo sapiens
46 COL8A1 1295
Two-hybrid Homo sapiens
47 KRAS 3845
Proximity Label-MS Homo sapiens
48 RAB5C 5878
Proximity Label-MS Homo sapiens
49 RHOB 388
Proximity Label-MS Homo sapiens
50 VENTX  
Two-hybrid Homo sapiens
51 CAV1 857
Proximity Label-MS Homo sapiens
52 FHL2 2274
Two-hybrid Homo sapiens
53 STK16 8576
Affinity Capture-MS Homo sapiens
54 PIP4K2B 8396
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 STX6 10228
Proximity Label-MS Homo sapiens
56 EFEMP2 30008
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which SPRY4 is involved
No pathways found





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