Gene description for SPRY4
Gene name sprouty homolog 4 (Drosophila)
Gene symbol SPRY4
Other names/aliases HH17
Species Homo sapiens
 Database cross references - SPRY4
ExoCarta ExoCarta_81848
Entrez Gene 81848
HGNC 15533
MIM 607984
UniProt Q9C004  
 SPRY4 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for SPRY4
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IEA
    multicellular organismal development GO:0007275 IEA
    negative regulation of MAP kinase activity GO:0043407 IEA
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    focal adhesion GO:0005925 IDA
    ruffle membrane GO:0032587 IEA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified SPRY4 in exosomes
Experiment ID 237
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
Experiment ID 126
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for SPRY4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TESK1 7016
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 RAF1 5894
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which SPRY4 is involved
No pathways found

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