Gene description for ASGR1
Gene name asialoglycoprotein receptor 1
Gene symbol ASGR1
Other names/aliases ASGPR
ASGPR1
CLEC4H1
HL-1
Species Homo sapiens
 Database cross references - ASGR1
ExoCarta ExoCarta_432
Vesiclepedia VP_432
Entrez Gene 432
HGNC 742
MIM 108360
UniProt P07306  
 ASGR1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
 Gene ontology annotations for ASGR1
Molecular Function
    asialoglycoprotein receptor activity GO:0004873 IEA
    protein binding GO:0005515 IPI
    D-mannose binding GO:0005537 IBA
    pattern recognition receptor activity GO:0038187 IBA
    identical protein binding GO:0042802 IEA
    fucose binding GO:0042806 IBA
    metal ion binding GO:0046872 IEA
Biological Process
    receptor-mediated endocytosis GO:0006898 TAS
    immune response GO:0006955 IBA
Subcellular Localization
    extracellular region GO:0005576 IEA
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IBA
 Experiment description of studies that identified ASGR1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ASGR1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Reconstituted Complex Homo sapiens
2 EDEM1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 RBBP6 5930
Two-hybrid Homo sapiens
4 KIR2DL3  
Two-hybrid Homo sapiens
5 HSPA5 3309
Affinity Capture-Western Homo sapiens
6 GPX8 493869
Two-hybrid Homo sapiens
7 FNDC9  
Two-hybrid Homo sapiens
8 PDZK1IP1 10158
Two-hybrid Homo sapiens
9 CANX 821
Affinity Capture-Western Homo sapiens
10 CPLX4  
Two-hybrid Homo sapiens
11 RNF19B  
Two-hybrid Homo sapiens
12 BLCAP  
Two-hybrid Homo sapiens
13 ABAT  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 FCGR1A  
Two-hybrid Homo sapiens
15 ERGIC3 51614
Two-hybrid Homo sapiens
16 ATP1B4  
Two-hybrid Homo sapiens
17 CERS2 29956
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
18 PTPN2 5771
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 IGFBP5 3488
Two-hybrid Homo sapiens
20 FBXO6 26270
Affinity Capture-Western Homo sapiens
21 SYVN1 84447
Affinity Capture-Western Homo sapiens
22 CLDN7 1366
Two-hybrid Homo sapiens
23 HIVEP1 3096
Two-hybrid Homo sapiens
24 CREB3L1  
Two-hybrid Homo sapiens
25 LAMTOR5 10542
Two-hybrid Homo sapiens
26 SUSD3 203328
Two-hybrid Homo sapiens
27 GALNT12 79695
Affinity Capture-MS Homo sapiens
28 AMIGO1  
Two-hybrid Homo sapiens
29 LEUTX  
Two-hybrid Homo sapiens
30 FAM209A 200232
Two-hybrid Homo sapiens
31 CLEC4G  
Two-hybrid Homo sapiens
32 MCEMP1  
Two-hybrid Homo sapiens
33 SPRY4 81848
Affinity Capture-MS Homo sapiens
34 HIBADH 11112
Two-hybrid Homo sapiens
35 C10orf54 64115
Two-hybrid Homo sapiens
36 SLC10A1  
Two-hybrid Homo sapiens
37 TMEM194A  
Two-hybrid Homo sapiens
38 VKORC1 79001
Two-hybrid Homo sapiens
39 AHNAK2 113146
Two-hybrid Homo sapiens
40 APH1A 51107
Two-hybrid Homo sapiens
41 GOLM1 51280
Affinity Capture-MS Homo sapiens
42 GALNT5 11227
Affinity Capture-MS Homo sapiens
43 NLK  
Two-hybrid Homo sapiens
44 TMX2 51075
Two-hybrid Homo sapiens
45 PLVAP 83483
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ASGR1 is involved
PathwayEvidenceSource
Asparagine N-linked glycosylation TAS Reactome
Metabolism of proteins TAS Reactome
Post-translational protein modification TAS Reactome





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