Gene description for GOLM1
Gene name golgi membrane protein 1
Gene symbol GOLM1
Other names/aliases C9orf155
GOLPH2
GP73
HEL46
PSEC0257
bA379P1.3
Species Homo sapiens
 Database cross references - GOLM1
ExoCarta ExoCarta_51280
Vesiclepedia VP_51280
Entrez Gene 51280
HGNC 15451
MIM 606804
UniProt Q8NBJ4  
 GOLM1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for GOLM1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    nucleus organization GO:0006997 IEA
    regulation of lipid metabolic process GO:0019216 IEA
Subcellular Localization
    extracellular space GO:0005615 HDA
    endoplasmic reticulum lumen GO:0005788 TAS
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified GOLM1 in exosomes
1
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GOLM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC39A12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CSTL1  
Affinity Capture-MS Homo sapiens
3 CHRNA4  
Affinity Capture-MS Homo sapiens
4 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 NRSN1  
Affinity Capture-MS Homo sapiens
6 RPA2 6118
Two-hybrid Homo sapiens
7 EIF2B2 8892
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TMEM147 10430
Two-hybrid Homo sapiens
9 TMUB1  
Two-hybrid Homo sapiens
10 AIG1  
Two-hybrid Homo sapiens
11 VSTM1  
Two-hybrid Homo sapiens
12 SLC7A14  
Affinity Capture-MS Homo sapiens
13 TMPO 7112
Two-hybrid Homo sapiens
14 SERINC2 347735
Affinity Capture-MS Homo sapiens
15 KIAA1429 25962
Affinity Capture-MS Homo sapiens
16 HEATR3 55027
Affinity Capture-MS Homo sapiens
17 SLC13A5  
Two-hybrid Homo sapiens
18 RNF5  
Affinity Capture-MS Homo sapiens
19 CTLA4  
Affinity Capture-MS Homo sapiens
20 KCNQ2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SYNJ2 8871
Cross-Linking-MS (XL-MS) Homo sapiens
22 DCK 1633
Two-hybrid Homo sapiens
23 MED22  
Two-hybrid Homo sapiens
24 TMEM192 201931
Affinity Capture-MS Homo sapiens
25 GUCA2A  
Two-hybrid Homo sapiens
26 Gspt1 14852
Affinity Capture-MS Mus musculus
27 CLEC10A  
Affinity Capture-MS Homo sapiens
28 KCNA4  
Affinity Capture-MS Homo sapiens
29 INSIG2  
Two-hybrid Homo sapiens
30 EXTL3 2137
Affinity Capture-MS Homo sapiens
31 RABEP2 79874
Affinity Capture-MS Homo sapiens
32 ALG6  
Affinity Capture-MS Homo sapiens
33 TMUB2  
Two-hybrid Homo sapiens
34 IGFBP5 3488
Two-hybrid Homo sapiens
35 JAGN1 84522
Two-hybrid Homo sapiens
36 SLC39A9 55334
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 ASNA1 439
Two-hybrid Homo sapiens
38 CHRNB2  
Affinity Capture-MS Homo sapiens
39 CLDND2  
Two-hybrid Homo sapiens
40 RIPPLY2  
Two-hybrid Homo sapiens
41 NEU1 4758
Two-hybrid Homo sapiens
42 KCNA6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 GPR182  
Affinity Capture-MS Homo sapiens
44 RPL13A 23521
Two-hybrid Homo sapiens
45 SLC30A3  
Two-hybrid Homo sapiens
46 BCL2L1 598
Two-hybrid Homo sapiens
47 TMEM140  
Two-hybrid Homo sapiens
48 GEMIN7  
Two-hybrid Homo sapiens
49 HGFAC 3083
Two-hybrid Homo sapiens
50 ST8SIA2  
Affinity Capture-MS Homo sapiens
51 S100A2 6273
Affinity Capture-MS Homo sapiens
52 ST6GAL2  
Two-hybrid Homo sapiens
53 UST  
Affinity Capture-MS Homo sapiens
54 CDIPT 10423
Two-hybrid Homo sapiens
55 LAT 27040
Two-hybrid Homo sapiens
56 ABI3 51225
Two-hybrid Homo sapiens
57 IL31  
Affinity Capture-MS Homo sapiens
58 TTYH1  
Affinity Capture-MS Homo sapiens
59 CHRNA1  
Affinity Capture-MS Homo sapiens
60 GAST  
Two-hybrid Homo sapiens
61 LCN1 3933
Affinity Capture-MS Homo sapiens
62 CNPY3 10695
Two-hybrid Homo sapiens
63 HSD17B13  
Two-hybrid Homo sapiens
64 FTH1 2495
Two-hybrid Homo sapiens
65 C16orf92  
Affinity Capture-MS Homo sapiens
66 Dctn2 69654
Affinity Capture-MS Mus musculus
67 TMEM86B  
Two-hybrid Homo sapiens
68 SAAL1 113174
Affinity Capture-MS Homo sapiens
69 CHRNA9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 LACRT 90070
Affinity Capture-MS Homo sapiens
71 C1orf174  
Two-hybrid Homo sapiens
72 ATP2A1 487
Affinity Capture-MS Homo sapiens
73 ACKR2  
Affinity Capture-MS Homo sapiens
74 GLB1 2720
Two-hybrid Homo sapiens
75 ASPH 444
Two-hybrid Homo sapiens
76 PIGF  
Affinity Capture-MS Homo sapiens
77 Bub1b 12236
Affinity Capture-MS Mus musculus
78 GALNT15  
Two-hybrid Homo sapiens
79 NDUFV2 4729
Two-hybrid Homo sapiens
80 TMEM59 9528
Affinity Capture-MS Homo sapiens
81 KCNA5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 AQP9  
Affinity Capture-MS Homo sapiens
83 FGL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 MBOAT7 79143
Affinity Capture-MS Homo sapiens
85 KCNA2  
Affinity Capture-MS Homo sapiens
86 MIP  
Two-hybrid Homo sapiens
87 ASGR1 432
Affinity Capture-MS Homo sapiens
88 SIDT2 51092
Affinity Capture-MS Homo sapiens
89 PGRMC1 10857
Two-hybrid Homo sapiens
90 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
91 SIPA1L3  
Affinity Capture-MS Homo sapiens
92 SLC35B2 347734
Two-hybrid Homo sapiens
93 NINJ1 4814
Two-hybrid Homo sapiens
94 TSPAN15 23555
Affinity Capture-MS Homo sapiens
95 DCTN2 10540
Two-hybrid Homo sapiens
96 KCNE3  
Two-hybrid Homo sapiens
97 MMGT1 93380
Affinity Capture-MS Homo sapiens
98 SFTPC  
Two-hybrid Homo sapiens
99 SCN4A  
Affinity Capture-MS Homo sapiens
100 CLEC12B  
Affinity Capture-MS Homo sapiens
101 GRAMD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 EIF3J 8669
Two-hybrid Homo sapiens
103 SEC62 7095
Affinity Capture-MS Homo sapiens
104 KCNA10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 ATP2B2 491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 TMEM109 79073
Two-hybrid Homo sapiens
107 FUT9  
Two-hybrid Homo sapiens
108 GNB2L1 10399
Two-hybrid Homo sapiens
109 SLCO3A1 28232
Two-hybrid Homo sapiens
110 CLDN4 1364
Two-hybrid Homo sapiens
111 SLC7A1 6541
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 ANKRD46 157567
Two-hybrid Homo sapiens
113 GNG8  
Affinity Capture-MS Homo sapiens
114 C1QL4  
Two-hybrid Homo sapiens
115 LOC100507537  
Two-hybrid Homo sapiens
116 SYT1 6857
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here