Gene description for SLC35B2
Gene name solute carrier family 35 (adenosine 3'-phospho 5'-phosphosulfate transporter), member B2
Gene symbol SLC35B2
Other names/aliases PAPST1
SLL
UGTrel4
Species Homo sapiens
 Database cross references - SLC35B2
ExoCarta ExoCarta_347734
Entrez Gene 347734
HGNC 16872
MIM 610788
UniProt Q8TB61  
 SLC35B2 identified in exosomes derived from the following tissue/cell type
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SLC35B2
Molecular Function
    signal transducer activity GO:0004871 IMP
    3'-phosphoadenosine 5'-phosphosulfate transmembrane transporter activity GO:0046964 IDA
Biological Process
    xenobiotic metabolic process GO:0006805 TAS
    carbohydrate metabolic process GO:0005975 TAS
    small molecule metabolic process GO:0044281 TAS
    3'-phosphoadenosine 5'-phosphosulfate biosynthetic process GO:0050428 TAS
    transmembrane transport GO:0055085 TAS
    3'-phosphoadenosine 5'-phosphosulfate transport GO:0046963 IDA
    signal transduction GO:0007165 IMP
    3'-phosphoadenosine 5'-phosphosulfate metabolic process GO:0050427 TAS
    3'-phospho-5'-adenylyl sulfate transmembrane transport GO:1902559 IDA
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IMP
    glycosaminoglycan metabolic process GO:0030203 TAS
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    Golgi apparatus GO:0005794 IDA
    membrane GO:0016020 IDA
    integral component of membrane GO:0016021 NAS
 Experiment description of studies that identified SLC35B2 in exosomes
1
Experiment ID 266
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✘ EV Membrane markers
✔
CANX|HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Wild type
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 268
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✘ EV Membrane markers
✔
CANX|HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx-null HBV replicon plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SLC35B2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which SLC35B2 is involved
PathwayEvidenceSource
Transport and synthesis of PAPS TAS Reactome
Transport and synthesis of PAPS TAS Reactome
Transport of nucleotide sugars TAS Reactome





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