Gene description for SLC35B2
Gene name solute carrier family 35 (adenosine 3'-phospho 5'-phosphosulfate transporter), member B2
Gene symbol SLC35B2
Other names/aliases PAPST1
SLL
UGTrel4
Species Homo sapiens
 Database cross references - SLC35B2
ExoCarta ExoCarta_347734
Vesiclepedia VP_347734
Entrez Gene 347734
HGNC 16872
MIM 610788
UniProt Q8TB61  
 SLC35B2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SLC35B2
Molecular Function
    protein binding GO:0005515 IPI
    antiporter activity GO:0015297 IEA
    3'-phosphoadenosine 5'-phosphosulfate transmembrane transporter activity GO:0046964 IBA
    3'-phosphoadenosine 5'-phosphosulfate transmembrane transporter activity GO:0046964 IDA
    3'-phosphoadenosine 5'-phosphosulfate transmembrane transporter activity GO:0046964 TAS
Biological Process
    chondroitin sulfate biosynthetic process GO:0030206 IMP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 HMP
    3'-phosphoadenosine 5'-phosphosulfate transport GO:0046963 IBA
    3'-phosphoadenosine 5'-phosphosulfate transport GO:0046963 IDA
    transmembrane transport GO:0055085 IBA
    5'-adenylyl sulfate transmembrane transport GO:1902558 TAS
    3'-phospho-5'-adenylyl sulfate transmembrane transport GO:1902559 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    Golgi membrane GO:0000139 IDA
    Golgi membrane GO:0000139 TAS
    endoplasmic reticulum membrane GO:0005789 IBA
    Golgi apparatus GO:0005794 IDA
    membrane GO:0016020 IDA
    membrane GO:0016020 NAS
 Experiment description of studies that identified SLC35B2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 266
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Wild type
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 268
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx-null HBV replicon plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SLC35B2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NRG1 3084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CLDN15  
Two-hybrid Homo sapiens
3 GNAI2 2771
Affinity Capture-MS Homo sapiens
4 GPM6A 2823
Affinity Capture-MS Homo sapiens
5 HSP90AB2P 391634
Affinity Capture-MS Homo sapiens
6 Tmed2 56334
Affinity Capture-MS Mus musculus
7 LYPD3 27076
Affinity Capture-MS Homo sapiens
8 AHSA1 10598
Affinity Capture-MS Homo sapiens
9 SLC15A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 ATP13A3 79572
Affinity Capture-MS Homo sapiens
11 SLC39A12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CCDC117  
Affinity Capture-MS Homo sapiens
13 CD69  
Two-hybrid Homo sapiens
14 TP53 7157
Affinity Capture-MS Homo sapiens
15 HSPA8 3312
Affinity Capture-MS Homo sapiens
16 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 HSP90AB4P 664618
Affinity Capture-MS Homo sapiens
18 RNF170 81790
Two-hybrid Homo sapiens
19 RAMP3  
Affinity Capture-MS Homo sapiens
20 LRRC55  
Affinity Capture-MS Homo sapiens
21 TMEM248 55069
Two-hybrid Homo sapiens
22 Bmpr1a  
Affinity Capture-MS Mus musculus
23 HTR3C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CMKLR1 1240
Affinity Capture-MS Homo sapiens
25 ASGR2  
Two-hybrid Homo sapiens
26 ERGIC3 51614
Two-hybrid Homo sapiens
27 BSCL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TSPAN15 23555
Affinity Capture-MS Homo sapiens
29 TYROBP  
Affinity Capture-MS Homo sapiens
30 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
31 CACNG6  
Affinity Capture-MS Homo sapiens
32 SLC7A14  
Affinity Capture-MS Homo sapiens
33 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
34 BAP1 8314
Negative Genetic Homo sapiens
35 OPRL1 4987
Affinity Capture-MS Homo sapiens
36 PDHA1 5160
Affinity Capture-MS Homo sapiens
37 FFAR1  
Affinity Capture-MS Homo sapiens
38 SLC22A9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 EDEM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 Sec24c  
Affinity Capture-MS Mus musculus
41 TOMM22 56993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 STOM 2040
Affinity Capture-MS Homo sapiens
43 SYP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 SLC18A2  
Affinity Capture-MS Homo sapiens
45 ACACA 31
Negative Genetic Homo sapiens
46 B3GAT1  
Proximity Label-MS Homo sapiens
47 EEF1A1P5 158078
Cross-Linking-MS (XL-MS) Homo sapiens
48 GOLM1 51280
Two-hybrid Homo sapiens
49 TMEM237 65062
Two-hybrid Homo sapiens
50 SPPL2B 56928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 CMTM5  
Affinity Capture-MS Homo sapiens
52 SLC39A5 283375
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 GPR182  
Affinity Capture-MS Homo sapiens
54 SLC24A3  
Affinity Capture-MS Homo sapiens
55 Ranbp2  
Affinity Capture-MS Mus musculus
56 CREB3L1  
Two-hybrid Homo sapiens
57 CDC37 11140
Affinity Capture-MS Homo sapiens
58 SERBP1 26135
Affinity Capture-MS Homo sapiens
59 ANXA4 307
Affinity Capture-MS Homo sapiens
60 CRELD1 78987
Affinity Capture-MS Homo sapiens
61 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 CLTC 1213
Affinity Capture-MS Homo sapiens
63 PBXIP1 57326
Affinity Capture-MS Homo sapiens
64 SLC22A4 6583
Affinity Capture-MS Homo sapiens
65 TEX29  
Two-hybrid Homo sapiens
66 SCN3B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 TARS 6897
Cross-Linking-MS (XL-MS) Homo sapiens
68 STIP1 10963
Affinity Capture-MS Homo sapiens
69 FPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 SLC2A9  
Affinity Capture-MS Homo sapiens
71 KIAA1644  
Two-hybrid Homo sapiens
72 LPAR1 1902
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 SLC7A1 6541
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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