Experiment description of studies that identified HSP90AB4P in sEVs

1 Experiment ID 1275 MISEV standards Biophysical techniques ✔ CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix Enriched markers ✔ PCNA|CANX|GAPDH|HSP90AA1|HSP90B1 Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36706192     Organism Homo sapiens Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." Journal name Sci Adv Publication year 2023 Sample Embryonic kidney cells Sample name HEK293T Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 1280 MISEV standards Biophysical techniques ✔ TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix Enriched markers ✔ PCNA|CANX|GAPDH|HSP90AA1|HSP90B1 Negative markers Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36706192     Organism Homo sapiens Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." Journal name Sci Adv Publication year 2023 Sample Embryonic kidney cells Sample name HEK293T - Fraction 5 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density 1.151 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

6 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

7 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

8 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for HSP90AB4P

Protein Interactor ExoCarta ID Identification method PubMed Species 1 ATP6V1B2 526 Cross-Linking-MS (XL-MS) Homo sapiens 2 CDK3 1018 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 3 HNRNPA0 10949 Cross-Linking-MS (XL-MS) Homo sapiens 4 FBXL4 26235 Affinity Capture-MS Homo sapiens 5 MYLK4   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 6 CPM 1368 Affinity Capture-MS Homo sapiens 7 ANKK1   Affinity Capture-MS Homo sapiens 8 TSSK6 83983 Affinity Capture-MS Homo sapiens 9 STYK1   Affinity Capture-MS Homo sapiens 10 THOC2 57187 Cross-Linking-MS (XL-MS) Homo sapiens 11 SLC2A1 6513 Cross-Linking-MS (XL-MS) Homo sapiens 12 RNMT 8731 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 RPS6KA4 8986 Affinity Capture-MS Homo sapiens 14 HSP90AB1 3326 Affinity Capture-MS Homo sapiens 15 UNC45B   Affinity Capture-MS Homo sapiens 16 DNAJB3 414061 Affinity Capture-MS Homo sapiens 17 DNAJB6 10049 Affinity Capture-MS Homo sapiens 18 AMHR2   Affinity Capture-MS Homo sapiens 19 KIF23 9493 Affinity Capture-MS Homo sapiens 20 YES1 7525 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 21 SMYD3 64754 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 22 AGO3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 23 TMEM184B   Affinity Capture-MS Homo sapiens 24 PRPS1 5631 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 25 LIAS   Affinity Capture-MS Homo sapiens 26 STIP1 10963 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 PLD2 5338 Affinity Capture-MS Homo sapiens 28 AMD1   Affinity Capture-MS Homo sapiens 29 MAP3K6   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 30 LIMK1 3984 Affinity Capture-MS Homo sapiens 31 AGO2 27161 Affinity Capture-MS Homo sapiens 32 CHMP4C 92421 Affinity Capture-MS Homo sapiens 33 PPP5C 5536 Affinity Capture-MS Homo sapiens 34 USP11 8237 Affinity Capture-MS Homo sapiens 35 PDGFRB 5159 Affinity Capture-MS Homo sapiens 36 RNF146   Affinity Capture-MS Homo sapiens 37 FYN 2534 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 38 ERP29 10961 Cross-Linking-MS (XL-MS) Homo sapiens 39 TTC9C 283237 Affinity Capture-MS Homo sapiens 40 ANAPC4 29945 Affinity Capture-MS Homo sapiens 41 Ksr1   Affinity Capture-MS Mus musculus 42 PRKACB 5567 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 43 REN   Affinity Capture-MS Homo sapiens 44 ANLN 54443 Affinity Capture-MS Homo sapiens 45 TXK   Affinity Capture-MS Homo sapiens 46 MRM1   Affinity Capture-MS Homo sapiens 47 CDK7 1022 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 48 ECT2 1894 Affinity Capture-MS Homo sapiens 49 TKTL1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 50 CDKL1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 51 AKT3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 52 FKBP8 23770 Affinity Capture-MS Homo sapiens 53 METTL21B   Affinity Capture-MS Homo sapiens 54 PLD5   Affinity Capture-MS Homo sapiens 55 HIST3H3 8290 Cross-Linking-MS (XL-MS) Homo sapiens 56 PAGE2 203569 Affinity Capture-MS Homo sapiens 57 GUCY2D   Affinity Capture-MS Homo sapiens 58 AURKB 9212 Affinity Capture-MS Homo sapiens 59 SLC35B2 347734 Affinity Capture-MS Homo sapiens 60 FKBP5 2289 Affinity Capture-MS Homo sapiens 61 AGO4   Affinity Capture-MS Homo sapiens 62 MREG   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 63 BAG1 573 Affinity Capture-MS Homo sapiens 64 CDKL4   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 65 SRMS   Affinity Capture-MS Homo sapiens 66 RAB28 9364 Affinity Capture-MS Homo sapiens 67 PLA2G1B   Affinity Capture-MS Homo sapiens 68 LLGL2 3993 Affinity Capture-MS Homo sapiens 69 MAP2K2 5605 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 70 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 71 MPLKIP   Affinity Capture-MS Homo sapiens 72 CCIN   Affinity Capture-MS Homo sapiens 73 DIDO1   Cross-Linking-MS (XL-MS) Homo sapiens 74 FOLR1 2348 Affinity Capture-MS Homo sapiens 75 AGO1   Affinity Capture-MS Homo sapiens 76 RUVBL2 10856 Affinity Capture-MS Homo sapiens 77 DUSP3 1845 Affinity Capture-MS Homo sapiens 78 LRRC28   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 79 CDC37 11140 Affinity Capture-MS Homo sapiens 80 IKBKG 8517 Affinity Capture-MS Homo sapiens 81 CCDC117   Affinity Capture-MS Homo sapiens 82 CDK15   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 83 FKBP4 2288 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 84 HSP90AA1 3320 Affinity Capture-MS Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens 85 SGK2   Affinity Capture-MS Homo sapiens 86 PLD3 23646 Affinity Capture-MS Homo sapiens 87 CDK13 8621 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 88 CNDP1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 89 PDIK1L   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 90 CDK4 1019 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 91 CDK9 1025 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 92 PINK1   Affinity Capture-MS Homo sapiens 93 STK35   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 94 STK11 6794 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 95 PTGES3 10728 Affinity Capture-MS Homo sapiens 96 PRKX 5613 Affinity Capture-MS Homo sapiens 97 PLD6   Affinity Capture-MS Homo sapiens 98 UBE3D   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 99 THOP1 7064 Cross-Linking-MS (XL-MS) Homo sapiens View the network

Pathways in which HSP90AB4P is involved

No pathways found