Gene description for DUSP3
Gene name dual specificity phosphatase 3
Gene symbol DUSP3
Other names/aliases VHR
Species Homo sapiens
 Database cross references - DUSP3
ExoCarta ExoCarta_1845
Entrez Gene 1845
HGNC 3069
MIM 600183
UniProt P51452  
 DUSP3 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for DUSP3
Molecular Function
    protein kinase binding GO:0019901 IDA
    protein tyrosine/serine/threonine phosphatase activity GO:0008138 IMP
    MAP kinase phosphatase activity GO:0033549 IMP
    protein tyrosine phosphatase activity GO:0004725 IDA
Biological Process
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    negative regulation of JNK cascade GO:0046329 IMP
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    inactivation of MAPK activity GO:0000188 IMP
    in utero embryonic development GO:0001701 IEA
    innate immune response GO:0045087 TAS
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    negative regulation of MAPK cascade GO:0043409 IMP
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    peptidyl-tyrosine dephosphorylation GO:0035335 IDA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IDA
    negative regulation of T cell activation GO:0050868 IDA
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    positive regulation of mitotic cell cycle GO:0045931 IMP
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    stress-activated MAPK cascade GO:0051403 TAS
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    negative regulation of T cell receptor signaling pathway GO:0050860 IDA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 IDA
    cytosol GO:0005829 IDA
    extracellular exosome GO:0070062 IDA
    cytoplasm GO:0005737 IDA
    immunological synapse GO:0001772 IDA
 Experiment description of studies that identified DUSP3 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DUSP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPK1 5594
Invivo Homo sapiens
Invitro Homo sapiens
2 EGFR 1956
Biochemical Activity Homo sapiens
3 MAPK3 5595
Invivo Homo sapiens
Invitro Homo sapiens
4 FGF7  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DUSP3 is involved
PathwayEvidenceSource
ERKs are inactivated TAS Reactome
ERKs are inactivated TAS Reactome





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