Gene description for CPSF2
Gene name cleavage and polyadenylation specific factor 2, 100kDa
Gene symbol CPSF2
Other names/aliases CPSF100
Species Homo sapiens
 Database cross references - CPSF2
ExoCarta ExoCarta_53981
Entrez Gene 53981
HGNC 2325
MIM 606028
UniProt Q9P2I0  
 CPSF2 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for CPSF2
Molecular Function
    protein binding GO:0005515 IPI
    RNA binding GO:0003723 IEA
Biological Process
    transcription from RNA polymerase II promoter GO:0006366 TAS
    mRNA export from nucleus GO:0006406 TAS
    mRNA cleavage GO:0006379 IEA
    mRNA 3'-end processing GO:0031124 TAS
    mRNA polyadenylation GO:0006378 IEA
    termination of RNA polymerase II transcription GO:0006369 TAS
    histone mRNA 3'-end processing GO:0006398 IDA
    mRNA splicing, via spliceosome GO:0000398 TAS
    RNA splicing GO:0008380 TAS
    gene expression GO:0010467 TAS
Subcellular Localization
    mRNA cleavage and polyadenylation specificity factor complex GO:0005847 IDA
    membrane GO:0016020 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified CPSF2 in exosomes
1
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for CPSF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CSTF2  
Affinity Capture-MS Homo sapiens
2 RNPS1 10921
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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