Gene description for SUPT5H
Gene name suppressor of Ty 5 homolog (S. cerevisiae)
Gene symbol SUPT5H
Other names/aliases SPT5
SPT5H
Tat-CT1
Species Homo sapiens
 Database cross references - SUPT5H
ExoCarta ExoCarta_6829
Entrez Gene 6829
HGNC 11469
MIM 602102
UniProt O00267  
 SUPT5H identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SUPT5H
Molecular Function
    protein heterodimerization activity GO:0046982 IPI
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
    enzyme binding GO:0019899 IPI
    chromatin binding GO:0003682 IEA
Biological Process
    gene expression GO:0010467 TAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IDA
    viral process GO:0016032 TAS
    cell cycle GO:0007049 NAS
    positive regulation of viral transcription GO:0050434 TAS
    transcription elongation from RNA polymerase II promoter GO:0006368 TAS
    negative regulation of DNA-templated transcription, elongation GO:0032785 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    positive regulation of macroautophagy GO:0016239 IMP
    DNA-templated transcription, elongation GO:0006354 IDA
    response to organic substance GO:0010033 TAS
    7-methylguanosine mRNA capping GO:0006370 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    single stranded viral RNA replication via double stranded DNA intermediate GO:0039692 NAS
    positive regulation of DNA-templated transcription, elongation GO:0032786 IDA
    chromatin remodeling GO:0006338 NAS
    negative regulation of mRNA polyadenylation GO:1900364 IMP
Subcellular Localization
    DSIF complex GO:0032044 IDA
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 IDA
 Experiment description of studies that identified SUPT5H in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SUPT5H
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PIN1 5300
Invitro Homo sapiens
2 HTATSF1 27336
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 PRMT5 10419
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 PRMT1 3276
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
5 SUPT4H1  
Affinity Capture-MS Homo sapiens
6 POLR2A 5430
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
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