Gene description for RNGTT
Gene name RNA guanylyltransferase and 5'-phosphatase
Gene symbol RNGTT
Other names/aliases CAP1A
HCE
HCE1
hCAP
Species Homo sapiens
 Database cross references - RNGTT
ExoCarta ExoCarta_8732
Vesiclepedia VP_8732
Entrez Gene 8732
HGNC 10073
MIM 603512
UniProt O60942  
 RNGTT identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Thymus 23844026    
 Gene ontology annotations for RNGTT
Molecular Function
    mRNA guanylyltransferase activity GO:0004484 IBA
    mRNA guanylyltransferase activity GO:0004484 IDA
    polynucleotide 5'-phosphatase activity GO:0004651 TAS
    phosphoprotein phosphatase activity GO:0004721 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    GTP binding GO:0005525 IEA
    RNA guanylyltransferase activity GO:0008192 IDA
    inorganic triphosphate phosphatase activity GO:0050355 IDA
    mRNA 5'-triphosphate monophosphatase activity GO:0140818 IEA
Biological Process
    7-methylguanosine mRNA capping GO:0006370 IBA
    7-methylguanosine mRNA capping GO:0006370 IDA
    7-methylguanosine mRNA capping GO:0006370 TAS
    RNA processing GO:0006396 IDA
Subcellular Localization
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified RNGTT in exosomes
1
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RNGTT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 RNGTT 8732
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 HECTD1 25831
Affinity Capture-MS Homo sapiens
5 RIT1 6016
Negative Genetic Homo sapiens
6 RECQL5  
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 MYCN  
Affinity Capture-MS Homo sapiens
9 POLR2G 5436
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SIRT6  
Affinity Capture-MS Homo sapiens
11 KPNA1 3836
Affinity Capture-MS Homo sapiens
12 MED17  
Affinity Capture-MS Homo sapiens
13 PPP4C 5531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 POLR2J  
Affinity Capture-MS Homo sapiens
15 POLR2H 5437
Affinity Capture-MS Homo sapiens
16 SMEK2  
Affinity Capture-MS Homo sapiens
17 IGHA1 3493
Affinity Capture-MS Homo sapiens
18 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
19 LY6G5B  
Affinity Capture-MS Homo sapiens
20 BRIX1 55299
Affinity Capture-MS Homo sapiens
21 RPAP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 RAN 5901
Affinity Capture-MS Homo sapiens
23 CRK 1398
Affinity Capture-MS Homo sapiens
24 KPNB1 3837
Affinity Capture-MS Homo sapiens
25 BRCA1 672
Affinity Capture-MS Homo sapiens
26 RPL37A 6168
Affinity Capture-MS Homo sapiens
27 RPRD2  
Affinity Capture-MS Homo sapiens
28 SNX18 112574
Affinity Capture-MS Homo sapiens
29 RNMT 8731
Reconstituted Complex Homo sapiens
30 RPRD1A  
Affinity Capture-MS Homo sapiens
31 CTDP1 9150
Affinity Capture-MS Homo sapiens
32 KPNA2 3838
Affinity Capture-MS Homo sapiens
33 POLR2D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CLUH 23277
Affinity Capture-MS Homo sapiens
35 METTL21B  
Affinity Capture-MS Homo sapiens
36 RPS19BP1  
Affinity Capture-MS Homo sapiens
37 SUPT5H 6829
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 MKI67  
Affinity Capture-MS Homo sapiens
39 SMEK1 55671
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 HNRNPH3 3189
Affinity Capture-MS Homo sapiens
41 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
42 SNX9 51429
Affinity Capture-MS Homo sapiens
43 TIMM50 92609
Affinity Capture-MS Homo sapiens
44 POLR2C 5432
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 KPNA4 3840
Affinity Capture-MS Homo sapiens
46 CAPZB 832
Affinity Capture-MS Homo sapiens
47 NUP50 10762
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 GADD45GIP1  
Affinity Capture-MS Homo sapiens
49 MED10  
Affinity Capture-MS Homo sapiens
50 BCAS2 10286
Affinity Capture-MS Homo sapiens
51 LACRT 90070
Affinity Capture-MS Homo sapiens
52 MYC  
Affinity Capture-MS Homo sapiens
53 RPA3 6119
Proximity Label-MS Homo sapiens
54 BARD1 580
Affinity Capture-MS Homo sapiens
55 NTRK1 4914
Affinity Capture-MS Homo sapiens
56 EGFR 1956
Negative Genetic Homo sapiens
57 EEF1D 1936
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 MED26  
Affinity Capture-MS Homo sapiens
59 SUPT4H1  
Affinity Capture-MS Homo sapiens
60 POLR2I 5438
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 PROL1  
Affinity Capture-MS Homo sapiens
62 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
63 POLR2L 5441
Affinity Capture-MS Homo sapiens
64 POLR2E 5434
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 DDRGK1 65992
Affinity Capture-MS Homo sapiens
66 C9orf72  
Affinity Capture-MS Homo sapiens
67 KRAS 3845
Negative Genetic Homo sapiens
68 RPL36AL 6166
Affinity Capture-MS Homo sapiens
69 POLR2M  
Affinity Capture-MS Homo sapiens
70 POLR2B 5431
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 SCAF4 57466
Affinity Capture-MS Homo sapiens
72 KPNA5 3841
Affinity Capture-MS Homo sapiens
73 NFATC2  
Affinity Capture-MS Homo sapiens
74 POLR2A 5430
Affinity Capture-MS Homo sapiens
75 CEBPA  
Proximity Label-MS Homo sapiens
Protein-peptide Homo sapiens
76 PPP4R2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 RPS10P5 93144
Affinity Capture-MS Homo sapiens
78 MED28  
Affinity Capture-MS Homo sapiens
79 INTU  
Affinity Capture-MS Homo sapiens
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