Experiment description of studies that identified FAM21A in exosomes

1 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

Protein-protein interactions for FAM21A

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PAAF1 80227 Co-fractionation Homo sapiens 2 GTSE1 51512 Affinity Capture-MS Homo sapiens 3 GTF2F1 2962 Co-fractionation Homo sapiens 4 SNX27 81609 Affinity Capture-MS Homo sapiens 5 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 6 HOOK1   Proximity Label-MS Homo sapiens 7 RPS27 6232 Cross-Linking-MS (XL-MS) Homo sapiens 8 C11orf31   Affinity Capture-MS Homo sapiens 9 AMOT   Proximity Label-MS Homo sapiens 10 ROCK1 6093 Co-fractionation Homo sapiens 11 AIFM1 9131 Cross-Linking-MS (XL-MS) Homo sapiens 12 NEDD4 4734 Co-fractionation Homo sapiens 13 LAMTOR1 55004 Proximity Label-MS Homo sapiens 14 KIAA0196 9897 Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens 15 RAB9A 9367 Proximity Label-MS Homo sapiens 16 XPO1 7514 Affinity Capture-MS Homo sapiens 17 DNAJC2 27000 Proximity Label-MS Homo sapiens 18 RAB5A 5868 Proximity Label-MS Homo sapiens 19 DNA2   Affinity Capture-MS Homo sapiens 20 Capza1 12340 Affinity Capture-MS Mus musculus 21 ACTC1 70 Proximity Label-MS Homo sapiens 22 VPS35 55737 Affinity Capture-MS Homo sapiens 23 CAPZA2 830 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 24 LAMP2 3920 Proximity Label-MS Homo sapiens 25 CAPZA1 829 Affinity Capture-MS Homo sapiens 26 DCTN2 10540 Proximity Label-MS Homo sapiens 27 RPS27L 51065 Cross-Linking-MS (XL-MS) Homo sapiens 28 HOOK3 84376 Proximity Label-MS Homo sapiens 29 SUPT5H 6829 Co-fractionation Homo sapiens 30 Wash   Affinity Capture-MS Mus musculus 31 KIAA1033 23325 Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens 32 SASS6 163786 Proximity Label-MS Homo sapiens 33 VPS29 51699 Affinity Capture-MS Homo sapiens 34 RAB4A 5867 Proximity Label-MS Homo sapiens 35 MLF1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 36 VPS26A 9559 Affinity Capture-MS Homo sapiens 37 CAPZB 832 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens 38 RPA3 6119 Proximity Label-MS Homo sapiens 39 CPLX2 10814 Affinity Capture-MS Homo sapiens 40 ANKFY1 51479 Co-fractionation Homo sapiens 41 ROCK2 9475 Co-fractionation Homo sapiens 42 VPS26B 112936 Affinity Capture-MS Homo sapiens 43 NTRK1 4914 Affinity Capture-MS Homo sapiens 44 RAB7A 7879 Proximity Label-MS Homo sapiens 45 ACTR3 10096 Proximity Label-MS Homo sapiens 46 GOLGA1   Proximity Label-MS Homo sapiens 47 FKBP15 23307 Affinity Capture-MS Homo sapiens 48 FAM21C 253725 Affinity Capture-MS Homo sapiens 49 TBC1D5 9779 Affinity Capture-MS Homo sapiens 50 WASH6P   Affinity Capture-MS Homo sapiens 51 RPA4   Proximity Label-MS Homo sapiens 52 ACTB 60 Proximity Label-MS Homo sapiens 53 PIN4 5303 Affinity Capture-MS Homo sapiens 54 CCDC53 51019 Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 55 AP3B1 8546 Co-fractionation Homo sapiens 56 RPLP0 6175 Co-fractionation Homo sapiens 57 DUSP16   Affinity Capture-MS Homo sapiens 58 WASH1 100287171 Co-fractionation Homo sapiens 59 WASH3P   Affinity Capture-MS Homo sapiens 60 IRF5   Proximity Label-MS Homo sapiens 61 PPM1G 5496 Co-fractionation Homo sapiens 62 ACTG1 71 Affinity Capture-MS Homo sapiens 63 TCEANC2   Affinity Capture-MS Homo sapiens 64 C17orf59 54785 Proximity Label-MS Homo sapiens 65 STX6 10228 Proximity Label-MS Homo sapiens 66 ACBD5 91452 Proximity Label-MS Homo sapiens 67 DNAJC13 23317 Affinity Capture-MS Homo sapiens 68 U2SURP 23350 Co-fractionation Homo sapiens View the network

Pathways in which FAM21A is involved

No pathways found