Gene description for FAM21C
Gene name family with sequence similarity 21, member C
Gene symbol FAM21C
Other names/aliases FAM21A
VPEF
Species Homo sapiens
 Database cross references - FAM21C
ExoCarta ExoCarta_253725
Vesiclepedia VP_253725
Entrez Gene 253725
HGNC 23414
MIM 613631
UniProt Q9Y4E1  
 FAM21C identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for FAM21C
Molecular Function
    protein binding GO:0005515 IPI
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IDA
    phosphatidylinositol-5-phosphate binding GO:0010314 IDA
    phosphatidylinositol-3-phosphate binding GO:0032266 IDA
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 IDA
    phosphatidylinositol-4-phosphate binding GO:0070273 IDA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IDA
    phosphatidylinositol phosphate binding GO:1901981 IBA
    retromer complex binding GO:1905394 IBA
    retromer complex binding GO:1905394 IDA
    retromer complex binding GO:1905394 IMP
Biological Process
    protein transport GO:0015031 IEA
    endosomal transport GO:0016197 NAS
    endocytic recycling GO:0032456 IMP
    regulation of Arp2/3 complex-mediated actin nucleation GO:0034315 NAS
    protein localization to endosome GO:0036010 IBA
    protein localization to endosome GO:0036010 IMP
    retrograde transport, endosome to Golgi GO:0042147 IBA
    retrograde transport, endosome to Golgi GO:0042147 IMP
    regulation of substrate adhesion-dependent cell spreading GO:1900024 IDA
    negative regulation of barbed-end actin filament capping GO:2000813 IDA
Subcellular Localization
    nucleolus GO:0005730 IDA
    endosome GO:0005768 IDA
    early endosome GO:0005769 IBA
    early endosome GO:0005769 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IEA
    early endosome membrane GO:0031901 NAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    WASH complex GO:0071203 IBA
    WASH complex GO:0071203 IDA
    WASH complex GO:0071203 NAS
 Experiment description of studies that identified FAM21C in exosomes
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for FAM21C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCDC93 54520
Affinity Capture-Western Homo sapiens
2 ATXN1 6310
Two-hybrid Homo sapiens
3 VPS35 55737
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
4 SNX27 81609
Affinity Capture-MS Homo sapiens
5 DCTN2 10540
Proximity Label-MS Homo sapiens
6 AMOT  
Proximity Label-MS Homo sapiens
7 FAS 355
Proximity Label-MS Homo sapiens
8 PSMC3 5702
Cross-Linking-MS (XL-MS) Homo sapiens
9 HORMAD2  
Affinity Capture-MS Homo sapiens
10 LAMTOR1 55004
Proximity Label-MS Homo sapiens
11 KIAA0196 9897
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
12 LAMP3  
Proximity Label-MS Homo sapiens
13 RAB5A 5868
Proximity Label-MS Homo sapiens
14 ACTR3 10096
Proximity Label-MS Homo sapiens
15 FKBP15 23307
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
16 Capza1 12340
Affinity Capture-MS Mus musculus
17 CAPZA1 829
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
18 CAPZA2 830
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 FAM21A 387680
Affinity Capture-MS Homo sapiens
20 LAMP2 3920
Proximity Label-MS Homo sapiens
21 C9orf78 51759
Affinity Capture-MS Homo sapiens
22 HOOK3 84376
Proximity Label-MS Homo sapiens
23 Wash  
Affinity Capture-MS Mus musculus
24 SASS6 163786
Proximity Label-MS Homo sapiens
25 VPS29 51699
Affinity Capture-MS Homo sapiens
26 RAB4A 5867
Proximity Label-MS Homo sapiens
27 VPS26A 9559
Affinity Capture-MS Homo sapiens
28 CAPZB 832
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
29 ING1  
Affinity Capture-MS Homo sapiens
30 WASH3P  
Affinity Capture-MS Homo sapiens
31 CEP135  
Proximity Label-MS Homo sapiens
32 CLTA 1211
Proximity Label-MS Homo sapiens
33 GOLGA1  
Proximity Label-MS Homo sapiens
34 RAB7A 7879
Proximity Label-MS Homo sapiens
35 ACTB 60
Proximity Label-MS Homo sapiens
36 CCDC22 28952
Affinity Capture-Western Homo sapiens
37 STIL  
Proximity Label-MS Homo sapiens
38 CCDC53 51019
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 WASH1 100287171
Co-fractionation Homo sapiens
40 RPA3 6119
Proximity Label-MS Homo sapiens
41 KIAA1033 23325
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
42 STX6 10228
Proximity Label-MS Homo sapiens
43 RAB9A 9367
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which FAM21C is involved
No pathways found





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