Gene description for VPS26B
Gene name vacuolar protein sorting 26 homolog B (S. pombe)
Gene symbol VPS26B
Other names/aliases Pep8b
Species Homo sapiens
 Database cross references - VPS26B
ExoCarta ExoCarta_112936
Vesiclepedia VP_112936
Entrez Gene 112936
HGNC 28119
MIM 610027
UniProt Q4G0F5  
 VPS26B identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for VPS26B
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    intracellular protein transport GO:0006886 IBA
    regulation of macroautophagy GO:0016241 TAS
    retrograde transport, endosome to Golgi GO:0042147 IBA
    retrograde transport, endosome to Golgi GO:0042147 ISS
    retrograde transport, endosome to Golgi GO:0042147 NAS
    cellular response to type II interferon GO:0071346 IEA
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 IEA
Subcellular Localization
    endosome GO:0005768 IBA
    early endosome GO:0005769 ISS
    late endosome GO:0005770 ISS
    cytosol GO:0005829 IEA
    retromer complex GO:0030904 IBA
    retromer complex GO:0030904 ISS
    retromer, cargo-selective complex GO:0030906 NAS
    phagocytic vesicle GO:0045335 IEA
    postsynaptic recycling endosome GO:0098837 IEA
    postsynaptic early endosome GO:0098842 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified VPS26B in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for VPS26B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNX1 6642
Co-fractionation Homo sapiens
2 GPM6A 2823
Affinity Capture-MS Homo sapiens
3 VPS35 55737
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 MRM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 COL4A5  
Affinity Capture-MS Homo sapiens
6 ANKRD27  
Affinity Capture-MS Homo sapiens
7 SNX3 8724
Affinity Capture-MS Homo sapiens
8 NPLOC4 55666
Two-hybrid Homo sapiens
9 FEM1A  
Affinity Capture-MS Homo sapiens
10 ZFC3H1 196441
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 KIAA0196 9897
Affinity Capture-MS Homo sapiens
12 CCDC102A 92922
Affinity Capture-MS Homo sapiens
13 MYH1  
Affinity Capture-MS Homo sapiens
14 RIN3  
Affinity Capture-MS Homo sapiens
15 ATE1 11101
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SNX27 81609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 S100A6 6277
Affinity Capture-MS Homo sapiens
18 FKBP15 23307
Affinity Capture-MS Homo sapiens
19 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
20 SNX16  
Affinity Capture-MS Homo sapiens
21 FAM21A 387680
Affinity Capture-MS Homo sapiens
22 LOXL3 84695
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 RAD51AP1  
Affinity Capture-MS Homo sapiens
24 LRRC73  
Affinity Capture-MS Homo sapiens
25 MTO1  
Affinity Capture-MS Homo sapiens
26 SDCCAG3  
Affinity Capture-MS Homo sapiens
27 DNAJB3 414061
Affinity Capture-MS Homo sapiens
28 FAXC  
Affinity Capture-MS Homo sapiens
29 CCDC102B  
Affinity Capture-MS Homo sapiens
30 NEK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 CAB39 51719
Co-fractionation Homo sapiens
32 VPS29 51699
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 NXF1 10482
Affinity Capture-RNA Homo sapiens
34 ETV6  
Affinity Capture-MS Homo sapiens
35 ABCD1 215
Affinity Capture-MS Homo sapiens
36 MRPS30 10884
Affinity Capture-MS Homo sapiens
37 VPS26A 9559
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
38 PPIL2  
Affinity Capture-MS Homo sapiens
39 C9orf156  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 MOV10 4343
Affinity Capture-RNA Homo sapiens
41 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
42 KIF5A 3798
Affinity Capture-MS Homo sapiens
43 ACTL8  
Affinity Capture-MS Homo sapiens
44 NTRK1 4914
Affinity Capture-MS Homo sapiens
45 TRIM7  
Affinity Capture-MS Homo sapiens
46 RAB7A 7879
Proximity Label-MS Homo sapiens
47 KXD1 79036
Affinity Capture-MS Homo sapiens
48 PNMA2  
Affinity Capture-MS Homo sapiens
49 TBC1D5 9779
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 ENO1 2023
Affinity Capture-RNA Homo sapiens
51 SNX2 6643
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
52 C21orf2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 SUPT5H 6829
Affinity Capture-MS Homo sapiens
54 UBA6 55236
Co-fractionation Homo sapiens
55 BTBD9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 GTSF1L  
Affinity Capture-MS Homo sapiens
57 GAPDH 2597
Cross-Linking-MS (XL-MS) Homo sapiens
58 KIAA1033 23325
Affinity Capture-MS Homo sapiens
59 TTF1  
Affinity Capture-MS Homo sapiens
60 KIF1B 23095
Affinity Capture-MS Homo sapiens
61 TXNDC11 51061
Affinity Capture-MS Homo sapiens
62 CHD9 80205
Affinity Capture-MS Homo sapiens
63 MYCBP2 23077
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which VPS26B is involved
No pathways found





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