Gene description for KIF5A
Gene name kinesin family member 5A
Gene symbol KIF5A
Other names/aliases D12S1889
MY050
NKHC
SPG10
Species Homo sapiens
 Database cross references - KIF5A
ExoCarta ExoCarta_3798
Vesiclepedia VP_3798
Entrez Gene 3798
HGNC 6323
MIM 602821
UniProt Q12840  
 KIF5A identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for KIF5A
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IEA
    motor activity GO:0003774 TAS
    ATP-dependent microtubule motor activity, plus-end-directed GO:0008574 IBA
Biological Process
    blood coagulation GO:0007596 TAS
    synaptic transmission GO:0007268 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    microtubule-based movement GO:0007018 TAS
    protein localization GO:0008104 IBA
    axon guidance GO:0007411 IBA
    cytoskeleton-dependent intracellular transport GO:0030705 IBA
    metabolic process GO:0008152 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 TAS
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 IEA
    kinesin complex GO:0005871 IEA
    membrane GO:0016020 IDA
    microtubule GO:0005874 IEA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified KIF5A in exosomes
1
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for KIF5A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TP53BP2  
Two-hybrid Homo sapiens
2 TSG101 7251
Two-hybrid Homo sapiens
3 KLC2 64837
Affinity Capture-Western Homo sapiens
4 ITSN1 6453
Two-hybrid Homo sapiens
5 YAP1 10413
Two-hybrid Homo sapiens
6 DTNB 1838
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 KLC1 3831
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which KIF5A is involved
PathwayEvidenceSource
Insulin processing TAS Reactome
Kinesins TAS Reactome
MHC class II antigen presentation TAS Reactome
RHO GTPases activate KTN1 TAS Reactome





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