Gene description for KIF11
Gene name kinesin family member 11
Gene symbol KIF11
Other names/aliases EG5
HKSP
KNSL1
MCLMR
TRIP5
Species Homo sapiens
 Database cross references - KIF11
ExoCarta ExoCarta_3832
Entrez Gene 3832
HGNC 6388
MIM 148760
UniProt P52732  
 KIF11 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for KIF11
Molecular Function
    ATP-dependent microtubule motor activity, plus-end-directed GO:0008574 IBA
    ATP binding GO:0005524 IEA
    protein kinase binding GO:0019901 IPI
    protein complex binding GO:0032403 IDA
    microtubule binding GO:0008017 IEA
    microtubule motor activity GO:0003777 TAS
Biological Process
    microtubule-based movement GO:0007018 TAS
    spindle organization GO:0007051 IMP
    chromosome segregation GO:0007059 IBA
    mitotic centrosome separation GO:0007100 IEA
    mitotic spindle organization GO:0007052 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 TAS
    mitotic spindle assembly GO:0090307 IMP
    mitotic nuclear division GO:0007067 TAS
    cell division GO:0051301 IEA
    metabolic process GO:0008152 TAS
    blood coagulation GO:0007596 TAS
    regulation of mitotic centrosome separation GO:0046602 IMP
Subcellular Localization
    spindle microtubule GO:0005876 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IDA
    spindle pole GO:0000922 IDA
    kinesin complex GO:0005871 IEA
    microtubule GO:0005874 IDA
    spindle GO:0005819 IDA
 Experiment description of studies that identified KIF11 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for KIF11
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DCTN1 1639
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
2 Thrb  
Two-hybrid Rattus norvegicus
3 KIF11 3832
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 NCOR1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which KIF11 is involved
PathwayEvidenceSource
Kinesins TAS Reactome
MHC class II antigen presentation TAS Reactome





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