Gene description for ACSL5
Gene name acyl-CoA synthetase long-chain family member 5
Gene symbol ACSL5
Other names/aliases ACS2
ACS5
FACL5
Species Homo sapiens
 Database cross references - ACSL5
ExoCarta ExoCarta_51703
Entrez Gene 51703
HGNC 16526
MIM 605677
UniProt Q9ULC5  
 ACSL5 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for ACSL5
Molecular Function
    long-chain fatty acid-CoA ligase activity GO:0004467 IEA
    ATP binding GO:0005524 IEA
Biological Process
    triglyceride biosynthetic process GO:0019432 TAS
    regulation of extrinsic apoptotic signaling pathway GO:2001236 IDA
    cellular lipid metabolic process GO:0044255 TAS
    long-chain fatty-acyl-CoA biosynthetic process GO:0035338 TAS
    small molecule metabolic process GO:0044281 TAS
    long-chain fatty acid metabolic process GO:0001676 IEA
Subcellular Localization
    mitochondrion GO:0005739 IDA
    integral component of membrane GO:0016021 IEA
    mitochondrial outer membrane GO:0005741 IEA
    membrane GO:0016020 IDA
    mitochondrial inner membrane GO:0005743 IEA
    endoplasmic reticulum GO:0005783 IDA
    nucleus GO:0005634 IDA
    nucleolus GO:0005730 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified ACSL5 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ACSL5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ACSL5 is involved
PathwayEvidenceSource
Synthesis of very long-chain fatty acyl-CoAs TAS Reactome





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