Gene description for EPT1
Gene name ethanolaminephosphotransferase 1
Gene symbol EPT1
Other names/aliases SELI
SEPI
Species Homo sapiens
 Database cross references - EPT1
ExoCarta ExoCarta_85465
Vesiclepedia VP_85465
Entrez Gene 85465
HGNC 29361
MIM 607915
UniProt Q9C0D9  
 EPT1 identified in sEVs derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for EPT1
Molecular Function
    ethanolaminephosphotransferase activity GO:0004307 IBA
    ethanolaminephosphotransferase activity GO:0004307 IDA
    ethanolaminephosphotransferase activity GO:0004307 IMP
    ethanolaminephosphotransferase activity GO:0004307 ISS
    metal ion binding GO:0046872 IEA
Biological Process
    phosphatidylethanolamine biosynthetic process GO:0006646 IBA
    phosphatidylethanolamine biosynthetic process GO:0006646 IDA
    phosphatidylethanolamine biosynthetic process GO:0006646 IEA
    phosphatidylethanolamine biosynthetic process GO:0006646 IMP
    ether lipid biosynthetic process GO:0008611 ISS
    myelination GO:0042552 ISS
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    Golgi apparatus GO:0005794 IBA
 Experiment description of studies that identified EPT1 in sEVs
1
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for EPT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CSNK2B 1460
Two-hybrid Homo sapiens
2 RPA2 6118
Proximity Label-MS Homo sapiens
3 APPBP2  
Two-hybrid Homo sapiens
4 SERBP1 26135
Affinity Capture-MS Homo sapiens
5 C12orf49  
Negative Genetic Homo sapiens
6 TMED10 10972
Affinity Capture-MS Homo sapiens
7 COPB2 9276
Affinity Capture-MS Homo sapiens
8 EWSR1 2130
Two-hybrid Homo sapiens
9 RAB2A 5862
Affinity Capture-MS Homo sapiens
10 VAPB 9217
Affinity Capture-MS Homo sapiens
11 RPA3 6119
Proximity Label-MS Homo sapiens
12 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
13 CANX 821
Affinity Capture-MS Homo sapiens
14 VAPA 9218
Affinity Capture-MS Homo sapiens
15 COIL  
Proximity Label-MS Homo sapiens
16 ACSL5 51703
Two-hybrid Homo sapiens
17 PPAT 5471
Two-hybrid Homo sapiens
18 DDX58 23586
Affinity Capture-RNA Homo sapiens
19 CLPP 8192
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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