Gene description for AP3M1
Gene name adaptor-related protein complex 3, mu 1 subunit
Gene symbol AP3M1
Other names/aliases -
Species Homo sapiens
 Database cross references - AP3M1
ExoCarta ExoCarta_26985
Vesiclepedia VP_26985
Entrez Gene 26985
HGNC 569
MIM 610366
UniProt Q9Y2T2  
 AP3M1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for AP3M1
Molecular Function
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IPI
Biological Process
    protein targeting to lysosome GO:0006622 TAS
    endocytosis GO:0006897 IBA
    anterograde axonal transport GO:0008089 ISS
    vesicle-mediated transport GO:0016192 NAS
    clathrin-coated vesicle cargo loading, AP-3-mediated GO:0035654 NAS
    intracellular transport GO:0046907 NAS
    anterograde synaptic vesicle transport GO:0048490 ISS
    platelet dense granule organization GO:0060155 NAS
    postsynaptic neurotransmitter receptor internalization GO:0098884 IEA
    melanosome assembly GO:1903232 NAS
Subcellular Localization
    lysosome GO:0005764 TAS
    lysosomal membrane GO:0005765 HDA
    early endosome GO:0005769 NAS
    Golgi apparatus GO:0005794 IEA
    AP-3 adaptor complex GO:0030123 NAS
    clathrin adaptor complex GO:0030131 IEA
    cytoplasmic vesicle membrane GO:0030659 IEA
    cytoplasmic vesicle GO:0031410 IBA
    postsynaptic recycling endosome GO:0098837 IEA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified AP3M1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AP3M1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGOLN2 10618
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 CAMKV 79012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 UBE2H 7328
Affinity Capture-MS Homo sapiens
4 VTI1B 10490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 COIL  
Proximity Label-MS Homo sapiens
6 SSR2  
Affinity Capture-MS Homo sapiens
7 ATG9A 79065
Proximity Label-MS Homo sapiens
8 PSMA4 5685
Co-fractionation Homo sapiens
9 PARD3 56288
Proximity Label-MS Homo sapiens
10 EPB41L5 57669
Affinity Capture-MS Homo sapiens
11 IL36RN  
Two-hybrid Homo sapiens
12 AP3M2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 DVL3 1857
Two-hybrid Homo sapiens
14 CCDC9  
Affinity Capture-MS Homo sapiens
15 KIAA1429 25962
Affinity Capture-MS Homo sapiens
16 WDR48 57599
Affinity Capture-MS Homo sapiens
17 AP3S2 10239
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 HLA-B 3106
Affinity Capture-MS Homo sapiens
19 LASP1 3927
Affinity Capture-MS Homo sapiens
20 AGO3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 ZNF460  
Affinity Capture-MS Homo sapiens
22 KLRC1  
Affinity Capture-MS Homo sapiens
23 EPHA1 2041
Affinity Capture-MS Homo sapiens
24 CRMP1 1400
Two-hybrid Homo sapiens
25 SNX27 81609
Affinity Capture-MS Homo sapiens
26 ARNT2  
Two-hybrid Homo sapiens
27 FAM20C 56975
Affinity Capture-MS Homo sapiens
28 PSMB3 5691
Co-fractionation Homo sapiens
29 LAMTOR1 55004
Proximity Label-MS Homo sapiens
30 LMAN1 3998
Proximity Label-MS Homo sapiens
31 NUP155 9631
Proximity Label-MS Homo sapiens
32 EPB41L3 23136
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 RUFY1 80230
Reconstituted Complex Homo sapiens
34 PARK2  
Affinity Capture-MS Homo sapiens
35 PLD1 5337
Affinity Capture-MS Homo sapiens
36 MCAM 4162
Proximity Label-MS Homo sapiens
37 AGTRAP 57085
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
38 DTNBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 RAP1B 5908
Affinity Capture-MS Homo sapiens
40 IDS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 VCL 7414
Affinity Capture-MS Homo sapiens
42 FAM9A  
Affinity Capture-MS Homo sapiens
43 SIPA1L2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
44 NTRK1 4914
Affinity Capture-MS Homo sapiens
45 Chmp4c  
Affinity Capture-MS Mus musculus
46 KIF11 3832
Affinity Capture-MS Homo sapiens
47 UBTF 7343
Co-fractionation Homo sapiens
48 APBA1  
Affinity Capture-MS Homo sapiens
49 AP3B1 8546
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 SLC12A4 6560
Two-hybrid Homo sapiens
51 BRD4 23476
Co-fractionation Homo sapiens
52 FGFBP1 9982
Affinity Capture-MS Homo sapiens
53 PALM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 EPB41L1 2036
Affinity Capture-MS Homo sapiens
55 SPAG16  
Two-hybrid Homo sapiens
56 PSMA3 5684
Co-fractionation Homo sapiens
57 XPO1 7514
Affinity Capture-MS Homo sapiens
58 CD79B 974
Affinity Capture-MS Homo sapiens
59 LRRC25  
Affinity Capture-MS Homo sapiens
60 EPB41L4A 64097
Proximity Label-MS Homo sapiens
61 FERMT2 10979
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 HDDC3  
Affinity Capture-MS Homo sapiens
63 NR3C1 2908
Proximity Label-MS Homo sapiens
64 EGFR 1956
Affinity Capture-MS Homo sapiens
65 PTK2 5747
Proximity Label-MS Homo sapiens
66 MYO1C 4641
Affinity Capture-MS Homo sapiens
67 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 NEFM 4741
Proximity Label-MS Homo sapiens
69 Uso1 56041
Affinity Capture-MS Mus musculus
70 PHF6  
Affinity Capture-MS Homo sapiens
71 CREB3L3  
Affinity Capture-MS Homo sapiens
72 PSMB5 5693
Co-fractionation Homo sapiens
73 LMNA 4000
Proximity Label-MS Homo sapiens
74 PSMB1 5689
Co-fractionation Homo sapiens
75 TMEM63A 9725
Affinity Capture-MS Homo sapiens
76 FLOT1 10211
Proximity Label-MS Homo sapiens
77 AP3S1 1176
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 CLPP 8192
Proximity Label-MS Homo sapiens
79 GRK5 2869
Affinity Capture-MS Homo sapiens
80 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
81 PARP1 142
Proximity Label-MS Homo sapiens
82 HLA-DMB 3109
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 RNF19B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 RSPH14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
85 PLCD3 113026
Affinity Capture-MS Homo sapiens
86 LCK 3932
Proximity Label-MS Homo sapiens
87 UTP23  
Affinity Capture-MS Homo sapiens
88 TRIM9  
Two-hybrid Homo sapiens
89 RABL6 55684
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 PSMA1 5682
Co-fractionation Homo sapiens
91 CD80 941
Affinity Capture-MS Homo sapiens
92 VAMP7 6845
Affinity Capture-Western Homo sapiens
93 SKAP1 8631
Affinity Capture-MS Homo sapiens
94 EDA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 DHX16 8449
Affinity Capture-MS Homo sapiens
96 TRIM23 373
Two-hybrid Homo sapiens
97 PSMA7 5688
Co-fractionation Homo sapiens
98 AP3B2 8120
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 SERBP1 26135
Affinity Capture-MS Homo sapiens
100 KRAS 3845
Proximity Label-MS Homo sapiens
101 Ufl1  
Affinity Capture-MS Mus musculus
102 C9orf72  
Affinity Capture-MS Homo sapiens
103 VASN 114990
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 AP3D1 8943
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 RPA2 6118
Proximity Label-MS Homo sapiens
106 RPA3 6119
Proximity Label-MS Homo sapiens
107 SEMA4B  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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