Gene description for AP3S2
Gene name adaptor-related protein complex 3, sigma 2 subunit
Gene symbol AP3S2
Other names/aliases AP3S3
sigma3b
Species Homo sapiens
 Database cross references - AP3S2
ExoCarta ExoCarta_10239
Entrez Gene 10239
HGNC 571
MIM 602416
UniProt P59780  
 AP3S2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for AP3S2
Molecular Function
    protein transporter activity GO:0008565 IEA
Biological Process
    anterograde synaptic vesicle transport GO:0048490 ISS
    anterograde axon cargo transport GO:0008089 ISS
    intracellular protein transport GO:0006886 IEA
Subcellular Localization
    AP-3 adaptor complex GO:0030123 IDA
    cytoplasmic vesicle membrane GO:0030659 IEA
    Golgi apparatus GO:0005794 IEA
 Experiment description of studies that identified AP3S2 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for AP3S2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AP3B1 8546
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
2 ARF1 375
Reconstituted Complex Homo sapiens
3 ARF6 382
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which AP3S2 is involved
No pathways found





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