Gene description for AP3S2
Gene name adaptor-related protein complex 3, sigma 2 subunit
Gene symbol AP3S2
Other names/aliases AP3S3
sigma3b
Species Homo sapiens
 Database cross references - AP3S2
ExoCarta ExoCarta_10239
Vesiclepedia VP_10239
Entrez Gene 10239
HGNC 571
MIM 602416
UniProt P59780  
 AP3S2 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for AP3S2
Biological Process
    intracellular protein transport GO:0006886 IEA
    Golgi to vacuole transport GO:0006896 IEA
    anterograde axonal transport GO:0008089 ISS
    synaptic vesicle coating GO:0016183 NAS
    vesicle-mediated transport GO:0016192 IBA
    vesicle-mediated transport GO:0016192 NAS
    clathrin-coated vesicle cargo loading, AP-3-mediated GO:0035654 NAS
    synaptic vesicle recycling GO:0036465 NAS
    intracellular transport GO:0046907 NAS
    anterograde synaptic vesicle transport GO:0048490 ISS
    platelet dense granule organization GO:0060155 NAS
    melanosome assembly GO:1903232 NAS
Subcellular Localization
    early endosome GO:0005769 NAS
    Golgi apparatus GO:0005794 IEA
    synaptic vesicle GO:0008021 IEA
    AP-3 adaptor complex GO:0030123 IDA
    AP-3 adaptor complex GO:0030123 NAS
    cytoplasmic vesicle membrane GO:0030659 IEA
    intracellular membrane-bounded organelle GO:0043231 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified AP3S2 in sEVs
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for AP3S2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 S100A6 6277
Affinity Capture-MS Homo sapiens
2 EPB41L3 23136
Affinity Capture-MS Homo sapiens
3 HUWE1 10075
Affinity Capture-MS Homo sapiens
4 TCIRG1 10312
Affinity Capture-MS Homo sapiens
5 AP3M1 26985
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
6 UBE2H 7328
Affinity Capture-MS Homo sapiens
7 RNF13 11342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 DTNBP1  
Affinity Capture-MS Homo sapiens
9 E2F4  
Two-hybrid Homo sapiens
10 AP3B1 8546
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
11 ATG9A 79065
Proximity Label-MS Homo sapiens
12 ARF6 382
Reconstituted Complex Homo sapiens
13 AP3D1 8943
Affinity Capture-Western Homo sapiens
14 AP3M2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 ARF1 375
Reconstituted Complex Homo sapiens
16 TMEM63A 9725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 LAMTOR1 55004
Proximity Label-MS Homo sapiens
18 SIDT2 51092
Affinity Capture-MS Homo sapiens
19 XPO1 7514
Affinity Capture-MS Homo sapiens
20 ARF4 378
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which AP3S2 is involved
No pathways found





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