Gene description for DNM3
Gene name dynamin 3
Gene symbol DNM3
Other names/aliases Dyna III
Species Homo sapiens
 Database cross references - DNM3
ExoCarta ExoCarta_26052
Vesiclepedia VP_26052
Entrez Gene 26052
HGNC 29125
MIM 611445
UniProt Q9UQ16  
 DNM3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Prostate cancer cells 25844599    
 Gene ontology annotations for DNM3
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISS
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    microtubule binding GO:0008017 IBA
    type 1 metabotropic glutamate receptor binding GO:0031798 IEA
    type 5 metabotropic glutamate receptor binding GO:0031802 IEA
    identical protein binding GO:0042802 IPI
    nitric-oxide synthase binding GO:0050998 IEA
    structural constituent of postsynapse GO:0099186 IEA
    protein serine/threonine kinase binding GO:0120283 IEA
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    endocytosis GO:0006897 ISS
    synapse assembly GO:0007416 ISS
    synaptic vesicle budding from presynaptic endocytic zone membrane GO:0016185 IBA
    receptor internalization GO:0031623 IBA
    filopodium assembly GO:0046847 ISS
    positive regulation of filopodium assembly GO:0051491 IEA
    negative regulation of dendritic spine morphogenesis GO:0061002 IEA
    postsynapse organization GO:0099173 IEA
    positive regulation of synaptic vesicle recycling GO:1903423 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    microtubule GO:0005874 IBA
    plasma membrane GO:0005886 IBA
    postsynaptic density GO:0014069 ISS
    axon GO:0030424 IEA
    synaptic cleft GO:0043083 IEA
    dendritic spine GO:0043197 ISS
    dendritic spine head GO:0044327 IEA
    synapse GO:0045202 IBA
    perinuclear region of cytoplasm GO:0048471 ISS
    apical tubulobulbar complex GO:0061828 IEA
    basal tubulobulbar complex GO:0061829 IEA
    extracellular exosome GO:0070062 HDA
    presynapse GO:0098793 IEA
    postsynaptic endocytic zone membrane GO:0098844 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified DNM3 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 275
MISEV standards
EM
Biophysical techniques
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
AIF
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for DNM3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HERC1 8925
Affinity Capture-MS Homo sapiens
2 SLC2A12 154091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 MND1  
Affinity Capture-MS Homo sapiens
4 GYPA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 MECP2 4204
Affinity Capture-MS Homo sapiens
6 DNAAF2  
Affinity Capture-MS Homo sapiens
7 IL1R2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 EFNA4  
Affinity Capture-MS Homo sapiens
9 TMEM9 252839
Affinity Capture-MS Homo sapiens
10 GYPB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
12 GRB2 2885
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 DNM2 1785
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 BTNL9  
Affinity Capture-MS Homo sapiens
15 THRB 7068
Affinity Capture-MS Homo sapiens
16 SNX33 257364
Affinity Capture-MS Homo sapiens
17 CDKAL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 GPM6A 2823
Affinity Capture-MS Homo sapiens
19 S100A6 6277
Affinity Capture-MS Homo sapiens
20 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 ALMS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 LAMP1 3916
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 TYROBP  
Affinity Capture-MS Homo sapiens
24 CMKLR1 1240
Affinity Capture-MS Homo sapiens
25 SERBP1 26135
Affinity Capture-MS Homo sapiens
26 EFNB1 1947
Affinity Capture-MS Homo sapiens
27 TMEM9B 56674
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TP73  
Affinity Capture-MS Homo sapiens
29 ZBTB2 57621
Affinity Capture-MS Homo sapiens
30 HCST  
Affinity Capture-MS Homo sapiens
31 DNM1 1759
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 FXYD6  
Affinity Capture-MS Homo sapiens
33 DNMBP 23268
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 MKI67  
Affinity Capture-MS Homo sapiens
36 RAPH1 65059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 HIST3H3 8290
Cross-Linking-MS (XL-MS) Homo sapiens
38 SPACA1 81833
Affinity Capture-MS Homo sapiens
39 SNX9 51429
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 COL1A1 1277
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 AMIGO1  
Affinity Capture-MS Homo sapiens
42 MRPS18C  
Affinity Capture-MS Homo sapiens
43 SH3GL3  
Affinity Capture-Western Homo sapiens
44 GBE1 2632
Co-fractionation Homo sapiens
45 ZBBX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 KIF11 3832
Affinity Capture-MS Homo sapiens
47 HAUS8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 VCP 7415
Affinity Capture-MS Homo sapiens
49 HECTD1 25831
Affinity Capture-MS Homo sapiens
50 SNX5 27131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 SLC39A7 7922
Affinity Capture-MS Homo sapiens
52 TSC22D2 9819
Affinity Capture-MS Homo sapiens
53 IGSF8 93185
Affinity Capture-MS Homo sapiens
54 GPR182  
Affinity Capture-MS Homo sapiens
55 NUFIP1  
Affinity Capture-MS Homo sapiens
56 THBS3 7059
Affinity Capture-MS Homo sapiens
57 SSUH2  
Affinity Capture-MS Homo sapiens
58 LYPD6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 SCN2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 SNX2 6643
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 GPR45  
Affinity Capture-MS Homo sapiens
62 RPE  
Co-fractionation Homo sapiens
63 TGM1 7051
Affinity Capture-MS Homo sapiens
64 KLRC1  
Affinity Capture-MS Homo sapiens
65 FXYD3 5349
Affinity Capture-MS Homo sapiens
66 FER 2241
Co-fractionation Homo sapiens
67 EFNB2 1948
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 SNX6 58533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 SNX1 6642
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 SH3GL2  
Affinity Capture-Western Homo sapiens
71 TBC1D15 64786
Affinity Capture-MS Homo sapiens
72 KIF2C 11004
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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