Gene description for MRTO4
Gene name mRNA turnover 4 homolog (S. cerevisiae)
Gene symbol MRTO4
Other names/aliases C1orf33
MRT4
dJ657E11.4
Species Homo sapiens
 Database cross references - MRTO4
ExoCarta ExoCarta_51154
Vesiclepedia VP_51154
Entrez Gene 51154
HGNC 18477
UniProt Q9UKD2  
 MRTO4 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MRTO4
Molecular Function
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
Biological Process
    ribosomal large subunit assembly GO:0000027 IEA
    nuclear-transcribed mRNA catabolic process GO:0000956 IBA
    rRNA processing GO:0006364 IBA
    ribosomal large subunit biogenesis GO:0042273 IBA
Subcellular Localization
    nucleolus GO:0005730 IBA
    cytoplasm GO:0005737 IEA
    preribosome, large subunit precursor GO:0030687 IBA
 Experiment description of studies that identified MRTO4 in exosomes
1
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MRTO4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Co-fractionation Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 UBE2H 7328
Affinity Capture-MS Homo sapiens
5 ISG15 9636
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
6 VAPA 9218
Co-fractionation Homo sapiens
7 KLF9 687
Proximity Label-MS Homo sapiens
8 RSL24D1  
Affinity Capture-Western Homo sapiens
9 LHX2  
Proximity Label-MS Homo sapiens
10 RPL37A 6168
Affinity Capture-MS Homo sapiens
11 SDAD1  
Co-fractionation Homo sapiens
12 NOP56 10528
Affinity Capture-MS Homo sapiens
13 RPL10 6134
Affinity Capture-MS Homo sapiens
14 KIF20A 10112
Affinity Capture-MS Homo sapiens
15 PTP4A1 7803
Affinity Capture-MS Homo sapiens
16 CAND1 55832
Affinity Capture-MS Homo sapiens
17 SOX2  
Proximity Label-MS Homo sapiens
18 DNAJB6 10049
Proximity Label-MS Homo sapiens
19 USP36  
Affinity Capture-MS Homo sapiens
20 RPL10A 4736
Co-fractionation Homo sapiens
21 WDR76  
Affinity Capture-MS Homo sapiens
22 KIF23 9493
Affinity Capture-MS Homo sapiens
23 DDX24 57062
Co-fractionation Homo sapiens
24 Htatsf1  
Two-hybrid Mus musculus
25 TERF2IP 54386
Two-hybrid Homo sapiens
26 FBXW7  
Affinity Capture-MS Homo sapiens
27 KLF6  
Proximity Label-MS Homo sapiens
28 FBL 2091
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
29 H2AFY2 55506
Affinity Capture-MS Homo sapiens
30 KLF15  
Proximity Label-MS Homo sapiens
31 OBSL1 23363
Affinity Capture-MS Homo sapiens
32 ADARB1 104
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 BMI1  
Affinity Capture-MS Homo sapiens
34 WDR12 55759
Co-fractionation Homo sapiens
35 MTMR2 8898
Affinity Capture-MS Homo sapiens
36 MYCN  
Affinity Capture-MS Homo sapiens
37 KIF14 9928
Affinity Capture-MS Homo sapiens
38 PES1 23481
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
39 BRIX1 55299
Co-fractionation Homo sapiens
40 E2F4  
Affinity Capture-MS Homo sapiens
41 AURKA 6790
Affinity Capture-MS Homo sapiens
42 RPL27 6155
Co-fractionation Homo sapiens
43 KIAA0020 9933
Co-fractionation Homo sapiens
44 FOXA1  
Affinity Capture-MS Homo sapiens
45 SUPT5H 6829
Co-fractionation Homo sapiens
46 NOP2 4839
Co-fractionation Homo sapiens
47 RPL5 6125
Co-fractionation Homo sapiens
48 NOC2L 26155
Co-fractionation Homo sapiens
49 ZFP41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 RPL31 6160
Co-fractionation Homo sapiens
51 IFI16 3428
Affinity Capture-MS Homo sapiens
52 Mki67 17345
Affinity Capture-MS Mus musculus
53 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 PML 5371
Affinity Capture-MS Homo sapiens
55 HECTD1 25831
Affinity Capture-MS Homo sapiens
56 CUL2 8453
Affinity Capture-MS Homo sapiens
57 CEBPA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Protein-peptide Homo sapiens
58 GTPBP4 23560
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 ANLN 54443
Affinity Capture-MS Homo sapiens
60 TLX2  
Proximity Label-MS Homo sapiens
61 ZBTB2 57621
Affinity Capture-MS Homo sapiens
62 XPO1 7514
Affinity Capture-Western Homo sapiens
63 RC3H2  
Affinity Capture-MS Homo sapiens
64 ECT2 1894
Affinity Capture-MS Homo sapiens
65 SIRT7  
Affinity Capture-MS Homo sapiens
66 ZNF622 90441
Co-fractionation Homo sapiens
67 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 EGFR 1956
Affinity Capture-MS Homo sapiens
69 EIF2S2 8894
Co-fractionation Homo sapiens
70 RRP15  
Co-fractionation Homo sapiens
71 FN1 2335
Affinity Capture-MS Homo sapiens
72 SOX15  
Proximity Label-MS Homo sapiens
73 EFTUD2 9343
Co-fractionation Homo sapiens
74 MRPL1  
Co-fractionation Homo sapiens
75 EBNA1BP2 10969
Co-fractionation Homo sapiens
76 MYC  
Affinity Capture-MS Homo sapiens
77 RPA3 6119
Proximity Label-MS Homo sapiens
78 MAGEB10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 DNAJC9 23234
Proximity Label-MS Homo sapiens
80 NHP2L1 4809
Co-fractionation Homo sapiens
81 NMD3 51068
Affinity Capture-Western Homo sapiens
82 DDRGK1 65992
Affinity Capture-MS Homo sapiens
83 RNF2  
Affinity Capture-MS Homo sapiens
84 ARID1A 8289
Negative Genetic Homo sapiens
85 Rpl35 66489
Affinity Capture-MS Mus musculus
86 FTSJ3 117246
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
87 GRK5 2869
Affinity Capture-MS Homo sapiens
88 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
89 RARA 5914
Affinity Capture-MS Homo sapiens
90 HDGF 3068
Affinity Capture-MS Homo sapiens
91 FBXL6  
Affinity Capture-MS Homo sapiens
92 NOL10  
Co-fractionation Homo sapiens
93 MNDA 4332
Affinity Capture-MS Homo sapiens
94 ZNF746  
Affinity Capture-MS Homo sapiens
95 MEN1 4221
Affinity Capture-MS Homo sapiens
96 NSA2  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
97 DDX58 23586
Affinity Capture-RNA Homo sapiens
98 MKI67  
Affinity Capture-MS Homo sapiens
99 NIFK 84365
Co-fractionation Homo sapiens
100 UFL1 23376
Affinity Capture-MS Homo sapiens
101 KRR1 11103
Co-fractionation Homo sapiens
102 XPO5 57510
Affinity Capture-Western Homo sapiens
103 CHMP4C 92421
Affinity Capture-MS Homo sapiens
104 NCL 4691
Affinity Capture-MS Homo sapiens
105 CAD 790
Affinity Capture-MS Homo sapiens
106 Rrbp1  
Affinity Capture-MS Mus musculus
107 GRSF1 2926
Proximity Label-MS Homo sapiens
108 RPL23A 6147
Affinity Capture-Western Homo sapiens
109 KRAS 3845
Negative Genetic Homo sapiens
110 HNRNPU 3192
Affinity Capture-MS Homo sapiens
111 RC3H1 149041
Affinity Capture-MS Homo sapiens
112 DDX56  
Co-fractionation Homo sapiens
113 EP300 2033
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MRTO4 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here