Gene description for LIMK1
Gene name LIM domain kinase 1
Gene symbol LIMK1
Other names/aliases LIMK
LIMK-1
Species Homo sapiens
 Database cross references - LIMK1
ExoCarta ExoCarta_3984
Vesiclepedia VP_3984
Entrez Gene 3984
HGNC 6613
MIM 601329
UniProt P53667  
 LIMK1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for LIMK1
Molecular Function
    protein kinase activity GO:0004672 NAS
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 TAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    heat shock protein binding GO:0031072 IDA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    metal ion binding GO:0046872 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 IMP
    signal transduction GO:0007165 TAS
    Rho protein signal transduction GO:0007266 TAS
    nervous system development GO:0007399 TAS
    actin cytoskeleton organization GO:0030036 IBA
    positive regulation of actin filament bundle assembly GO:0032233 IDA
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    stress fiber assembly GO:0043149 IEA
    positive regulation of axon extension GO:0045773 ISS
    axon extension GO:0048675 IEA
    negative regulation of ubiquitin-protein transferase activity GO:0051444 IDA
    positive regulation of stress fiber assembly GO:0051496 IBA
Subcellular Localization
    male germ cell nucleus GO:0001673 IEA
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    focal adhesion GO:0005925 IEA
    membrane GO:0016020 IEA
    nuclear speck GO:0016607 IDA
    lamellipodium GO:0030027 ISS
    neuron projection GO:0043005 IBA
    neuron projection GO:0043005 ISS
    postsynapse GO:0098794 IDA
    postsynapse GO:0098794 IMP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified LIMK1 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for LIMK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATP1A2 477
Affinity Capture-MS Homo sapiens
2 NRG1 3084
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 LIMK1 3984
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 SH3PXD2B 285590
Affinity Capture-MS Homo sapiens
5 BAD  
Affinity Capture-MS Homo sapiens
6 RXFP2  
Affinity Capture-MS Homo sapiens
7 LAMTOR1 55004
Affinity Capture-MS Homo sapiens
8 PAK1 5058
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 TADA3  
Affinity Capture-MS Homo sapiens
10 ERBB2IP 55914
Affinity Capture-MS Homo sapiens
11 HSP90AB4P 664618
Affinity Capture-MS Homo sapiens
12 CDC37 11140
Affinity Capture-MS Homo sapiens
13 MIIP 60672
Affinity Capture-MS Homo sapiens
14 SEPT14  
Affinity Capture-MS Homo sapiens
15 YWHAZ 7534
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
16 UXT 8409
Affinity Capture-MS Homo sapiens
17 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
18 PARK2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
19 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
20 CFL1 1072
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
21 DDX58 23586
Affinity Capture-RNA Homo sapiens
22 MKI67  
Affinity Capture-MS Homo sapiens
23 APP 351
Reconstituted Complex Homo sapiens
24 LATS1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 ITGA1 3672
Affinity Capture-MS Homo sapiens
26 C22orf15  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
27 LONP2 83752
Affinity Capture-MS Homo sapiens
28 POLR2D  
Affinity Capture-MS Homo sapiens
29 RRAS 6237
Affinity Capture-MS Homo sapiens
30 MKRN2 23609
Affinity Capture-RNA Homo sapiens
31 BMPR2 659
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
32 FYN 2534
Affinity Capture-MS Homo sapiens
33 PAK4 10298
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
34 KIF23 9493
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
35 CDKN1C  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
36 BLMH 642
Affinity Capture-MS Homo sapiens
37 OSBPL5 114879
Affinity Capture-MS Homo sapiens
38 HSPA4 3308
Affinity Capture-Western Homo sapiens
39 HIST1H1A 3024
Biochemical Activity Homo sapiens
40 HSP90AA5P 730211
Affinity Capture-MS Homo sapiens
41 BRAF  
Affinity Capture-MS Homo sapiens
42 RBFOX3  
Affinity Capture-MS Homo sapiens
43 WASH1 100287171
Two-hybrid Homo sapiens
44 LIMK2 3985
Biochemical Activity Homo sapiens
Negative Genetic Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
45 RAD54L 8438
Affinity Capture-MS Homo sapiens
46 MBP 4155
Biochemical Activity Homo sapiens
47 SRPK1 6732
Biochemical Activity Homo sapiens
48 SARNP 84324
Affinity Capture-MS Homo sapiens
49 RTEL1 51750
Affinity Capture-MS Homo sapiens
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