Gene description for LIMK1
Gene name LIM domain kinase 1
Gene symbol LIMK1
Other names/aliases LIMK
LIMK-1
Species Homo sapiens
 Database cross references - LIMK1
ExoCarta ExoCarta_3984
Vesiclepedia VP_3984
Entrez Gene 3984
HGNC 6613
MIM 601329
UniProt P53667  
 LIMK1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for LIMK1
Molecular Function
    protein heterodimerization activity GO:0046982 IEA
    protein binding GO:0005515 IPI
    protein kinase activity GO:0004672 NAS
    heat shock protein binding GO:0031072 IDA
    ATP binding GO:0005524 IEA
    zinc ion binding GO:0008270 IEA
    protein serine/threonine kinase activity GO:0004674 TAS
Biological Process
    protein phosphorylation GO:0006468 IMP
    actin cytoskeleton organization GO:0030036 TAS
    positive regulation of stress fiber assembly GO:0051496 IEA
    nervous system development GO:0007399 TAS
    axon guidance GO:0007411 TAS
    ephrin receptor signaling pathway GO:0048013 TAS
    negative regulation of ubiquitin-protein transferase activity GO:0051444 IDA
    signal transduction GO:0007165 TAS
    innate immune response GO:0045087 TAS
    positive regulation of actin filament bundle assembly GO:0032233 IDA
    Rho protein signal transduction GO:0007266 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    positive regulation of axon extension GO:0045773 ISS
Subcellular Localization
    focal adhesion GO:0005925 IEA
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 IDA
    neuron projection GO:0043005 ISS
 Experiment description of studies that identified LIMK1 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for LIMK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NRG1 3084
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 CFL1 1072
Invitro Homo sapiens
Biochemical Activity Homo sapiens
3 LIMK1 3984
Invivo Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
4 YWHAZ 7534
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
5 RNF12  
Invitro Homo sapiens
6 LIMK2 3985
Invivo Homo sapiens
Invitro Homo sapiens
7 PAK4 10298
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
8 CDKN1C  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 ROCK1 6093
Invivo Homo sapiens
Invitro Homo sapiens
10 PAK1 5058
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 BMPR2 659
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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