Gene description for RAD54L
Gene name RAD54-like (S. cerevisiae)
Gene symbol RAD54L
Other names/aliases HR54
RAD54A
hHR54
hRAD54
Species Homo sapiens
 Database cross references - RAD54L
ExoCarta ExoCarta_8438
Vesiclepedia VP_8438
Entrez Gene 8438
HGNC 9826
MIM 603615
UniProt Q92698  
 RAD54L identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for RAD54L
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    four-way junction helicase activity GO:0009378 IEA
    DNA translocase activity GO:0015616 IBA
    ATP hydrolysis activity GO:0016887 IEA
    double-stranded DNA helicase activity GO:0036121 IEA
    ATP-dependent DNA/DNA annealing activity GO:0036310 IDA
    metal ion binding GO:0046872 IEA
    forked DNA-dependent helicase activity GO:0061749 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    DNA repair GO:0006281 TAS
    DNA recombination GO:0006310 TAS
    chromatin remodeling GO:0006338 IEA
    reciprocal meiotic recombination GO:0007131 IBA
    determination of adult lifespan GO:0008340 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    response to ionizing radiation GO:0010212 IEA
    DNA duplex unwinding GO:0032508 IEA
    double-strand break repair via synthesis-dependent strand annealing GO:0045003 IBA
    meiotic cell cycle GO:0051321 TAS
    chromatin looping GO:0140588 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    protein-containing complex GO:0032991 IMP
 Experiment description of studies that identified RAD54L in exosomes
1
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for RAD54L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BAG6 7917
Affinity Capture-MS Homo sapiens
2 PTGES3 10728
Affinity Capture-MS Homo sapiens
3 LIMK1 3984
Affinity Capture-MS Homo sapiens
4 SGTB  
Affinity Capture-MS Homo sapiens
5 C12orf49  
Positive Genetic Homo sapiens
6 PTEN 5728
Negative Genetic Homo sapiens
7 NCK1 4690
Affinity Capture-MS Homo sapiens
8 HECW2  
Affinity Capture-MS Homo sapiens
9 WDYHV1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
10 Tecpr2  
Affinity Capture-MS Mus musculus
11 PFDN5 5204
Negative Genetic Homo sapiens
12 NDUFA9 4704
Negative Genetic Homo sapiens
13 VHL  
Negative Genetic Homo sapiens
View the network image/svg+xml
 Pathways in which RAD54L is involved
No pathways found





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