Gene description for RPS6KA4
Gene name ribosomal protein S6 kinase, 90kDa, polypeptide 4
Gene symbol RPS6KA4
Other names/aliases MSK2
RSK-B
Species Homo sapiens
 Database cross references - RPS6KA4
ExoCarta ExoCarta_8986
Vesiclepedia VP_8986
Entrez Gene 8986
HGNC 10433
MIM 603606
UniProt O75676  
 RPS6KA4 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for RPS6KA4
Molecular Function
    ATP binding GO:0005524 IDA
    magnesium ion binding GO:0000287 IEA
    mitogen-activated protein kinase p38 binding GO:0048273 IGI
    protein binding GO:0005515 IPI
    protein serine/threonine kinase activity GO:0004674 IMP
    ribosomal protein S6 kinase activity GO:0004711 NAS
Biological Process
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    regulation of transcription, DNA-templated GO:0006355 IDA
    histone phosphorylation GO:0016572 TAS
    inflammatory response GO:0006954 IEA
    positive regulation of CREB transcription factor activity GO:0032793 TAS
    intracellular signal transduction GO:0035556 IDA
    positive regulation of histone acetylation GO:0035066 IMP
    positive regulation of histone phosphorylation GO:0033129 IMP
    axon guidance GO:0007411 TAS
    histone H3-S10 phosphorylation GO:0043987 IMP
    interleukin-1-mediated signaling pathway GO:0070498 IMP
    histone H3-S28 phosphorylation GO:0043988 IMP
    negative regulation of cytokine production GO:0001818 TAS
    protein phosphorylation GO:0006468 IDA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 TAS
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified RPS6KA4 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RPS6KA4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FOS 2353
Biochemical Activity Homo sapiens
2 CREB1  
Biochemical Activity Homo sapiens
3 MAPK14 1432
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Phenotypic Enhancement Homo sapiens
4 MAPK3 5595
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which RPS6KA4 is involved
PathwayEvidenceSource
Recycling pathway of L1 TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here