Gene description for TSSK6
Gene name testis-specific serine kinase 6
Gene symbol TSSK6
Other names/aliases CT72
FKSG82
SSTK
TSSK4
Species Homo sapiens
 Database cross references - TSSK6
ExoCarta ExoCarta_83983
Vesiclepedia VP_83983
Entrez Gene 83983
HGNC 30410
MIM 610712
UniProt Q9BXA6  
 TSSK6 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for TSSK6
Molecular Function
    magnesium ion binding GO:0000287 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    protein-containing complex binding GO:0044877 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    spermatid development GO:0007286 IBA
    flagellated sperm motility GO:0030317 ISS
    sperm DNA condensation GO:0035092 ISS
Subcellular Localization
    nucleus GO:0005634 HDA
    sperm flagellum GO:0036126 ISS
    axonemal microtubule doublet inner sheath GO:0160110 ISS
 Experiment description of studies that identified TSSK6 in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for TSSK6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCT8 10694
Affinity Capture-MS Homo sapiens
2 CCT3 7203
Affinity Capture-MS Homo sapiens
3 HIST1H3A 8350
Biochemical Activity Homo sapiens
4 HIST1H1C  
Biochemical Activity Bos taurus
5 HSPA8 3312
Affinity Capture-MS Homo sapiens
6 CCT2 10576
Affinity Capture-MS Homo sapiens
7 CCT6A 908
Affinity Capture-MS Homo sapiens
8 ARFGAP2 84364
Affinity Capture-MS Homo sapiens
9 HSP90AB4P 664618
Affinity Capture-MS Homo sapiens
10 CDC37 11140
Affinity Capture-MS Homo sapiens
11 TCP1 6950
Affinity Capture-MS Homo sapiens
12 TMEM160 54958
Affinity Capture-MS Homo sapiens
13 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
14 CCT4 10575
Affinity Capture-MS Homo sapiens
15 HSP90AB3P 3327
Affinity Capture-MS Homo sapiens
16 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 HSP90AA5P 730211
Affinity Capture-MS Homo sapiens
18 APP 351
Reconstituted Complex Homo sapiens
19 CCT7 10574
Affinity Capture-MS Homo sapiens
20 CCT5 22948
Affinity Capture-MS Homo sapiens
21 FKBP5 2289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 HSP90AA4P 3323
Affinity Capture-MS Homo sapiens
23 TANGO6 79613
Affinity Capture-MS Homo sapiens
24 HSPA4 3308
Affinity Capture-MS Homo sapiens
25 MDM2  
Affinity Capture-MS Homo sapiens
26 MBP 4155
Biochemical Activity Homo sapiens
27 HIST2H2AC 8338
Biochemical Activity Homo sapiens
28 H2AFX 3014
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which TSSK6 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here