Gene description for CDK13
Gene name cyclin-dependent kinase 13
Gene symbol CDK13
Other names/aliases CDC2L
CDC2L5
CHED
hCDK13
Species Homo sapiens
 Database cross references - CDK13
ExoCarta ExoCarta_8621
Vesiclepedia VP_8621
Entrez Gene 8621
HGNC 1733
MIM 603309
UniProt Q14004  
 CDK13 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CDK13
Molecular Function
    RNA binding GO:0003723 HDA
    protein kinase activity GO:0004672 TAS
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    RNA polymerase II CTD heptapeptide repeat kinase activity GO:0008353 IBA
    RNA polymerase II CTD heptapeptide repeat kinase activity GO:0008353 IDA
    protein kinase binding GO:0019901 IEA
    cyclin binding GO:0030332 IBA
    cyclin binding GO:0030332 IPI
    protein serine kinase activity GO:0106310 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 kinase activity GO:0140833 IEA
    RNA polymerase II CTD heptapeptide repeat S2 kinase activity GO:0140834 IEA
    RNA polymerase II CTD heptapeptide repeat T4 kinase activity GO:0140835 IEA
    RNA polymerase II CTD heptapeptide repeat S5 kinase activity GO:0140836 IEA
    RNA polymerase II CTD heptapeptide repeat S7 kinase activity GO:0140837 IEA
Biological Process
    alternative mRNA splicing, via spliceosome GO:0000380 ISS
    alternative mRNA splicing, via spliceosome GO:0000380 TAS
    regulation of signal transduction GO:0009966 IDA
    hemopoiesis GO:0030097 IMP
    positive regulation of transcription elongation by RNA polymerase II GO:0032968 IBA
    positive regulation of transcription elongation by RNA polymerase II GO:0032968 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    regulation of cell cycle GO:0051726 IEA
    negative regulation of stem cell differentiation GO:2000737 IEA
Subcellular Localization
    cyclin K-CDK13 complex GO:0002945 IPI
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    cyclin/CDK positive transcription elongation factor complex GO:0008024 IBA
    nuclear speck GO:0016607 IDA
    nuclear cyclin-dependent protein kinase holoenzyme complex GO:0019908 ISS
    ficolin-1-rich granule lumen GO:1904813 TAS
 Experiment description of studies that identified CDK13 in exosomes
1
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CDK13
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNASEH1 246243
Affinity Capture-MS Homo sapiens
2 CCT3 7203
Affinity Capture-MS Homo sapiens
3 SRPK2 6733
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 YWHAE 7531
Affinity Capture-MS Homo sapiens
5 RPL13A 23521
Affinity Capture-MS Homo sapiens
6 ACTC1 70
Proximity Label-MS Homo sapiens
7 TUBB8 347688
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 DHX8 1659
Proximity Label-MS Homo sapiens
9 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PRPF3  
Co-fractionation Homo sapiens
11 PRPF40A 55660
Two-hybrid Homo sapiens
12 RPS3 6188
Affinity Capture-MS Homo sapiens
13 USP36  
Affinity Capture-MS Homo sapiens
14 CCNK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 DDX23 9416
Proximity Label-MS Homo sapiens
16 FBXO44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 UBE2O 63893
Affinity Capture-MS Homo sapiens
18 CHCHD2  
Affinity Capture-MS Homo sapiens
19 TRMT44  
Affinity Capture-MS Homo sapiens
20 CDK12 51755
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
21 MAPK14 1432
Co-fractionation Homo sapiens
22 ZNF2  
Affinity Capture-MS Homo sapiens
23 NEK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 KRT31 3881
Two-hybrid Homo sapiens
25 FBXO11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 ALK 238
Affinity Capture-MS Homo sapiens
27 SRP68 6730
Affinity Capture-MS Homo sapiens
28 CHMP4A 29082
Two-hybrid Homo sapiens
29 POLR2C 5432
Proximity Label-MS Homo sapiens
30 MYCN  
Affinity Capture-MS Homo sapiens
31 SERBP1 26135
Affinity Capture-MS Homo sapiens
32 SPSB3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 RB1 5925
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 PPM1B 5495
Co-fractionation Homo sapiens
35 HSP90AB4P 664618
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
37 RPL4 6124
Affinity Capture-MS Homo sapiens
38 SRSF3 6428
Affinity Capture-MS Homo sapiens
39 TUBA1A 7846
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SRSF6 6431
Affinity Capture-MS Homo sapiens
41 PURG  
Affinity Capture-MS Homo sapiens
42 TUBA1C 84790
Affinity Capture-MS Homo sapiens
43 BARD1 580
Affinity Capture-MS Homo sapiens
44 NTRK1 4914
Affinity Capture-MS Homo sapiens
45 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
46 BANF1 8815
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
47 RPA4  
Proximity Label-MS Homo sapiens
48 HECTD1 25831
Affinity Capture-MS Homo sapiens
49 RPLP0 6175
Affinity Capture-MS Homo sapiens
50 SRPK3  
Affinity Capture-MS Homo sapiens
51 MAGEB2 4113
Affinity Capture-MS Homo sapiens
52 CEBPA  
Proximity Label-MS Homo sapiens
Protein-peptide Homo sapiens
53 WDR46  
Affinity Capture-MS Homo sapiens
54 FGFBP1 9982
Affinity Capture-MS Homo sapiens
55 TUBB 203068
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
57 JPH4 84502
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 RC3H2  
Affinity Capture-MS Homo sapiens
59 SRSF4 6429
Affinity Capture-MS Homo sapiens
60 EEF2 1938
Affinity Capture-MS Homo sapiens
61 SRSF1 6426
Affinity Capture-MS Homo sapiens
62 SRSF5 6430
Affinity Capture-MS Homo sapiens
63 TUBB4B 10383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 EGFR 1956
Affinity Capture-MS Homo sapiens
65 HSP90AB3P 3327
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 HSP90AA5P 730211
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 TUBA4A 7277
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 FASN 2194
Positive Genetic Homo sapiens
69 FKBP5 2289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 HSP90AA4P 3323
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 PSPC1 55269
Affinity Capture-MS Homo sapiens
72 RPA3 6119
Proximity Label-MS Homo sapiens
73 GNB2 2783
Affinity Capture-MS Homo sapiens
74 RBM14-RBM4 100526737
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 DDX54 79039
Affinity Capture-MS Homo sapiens
76 RNF2  
Affinity Capture-MS Homo sapiens
77 H2AFB2  
Affinity Capture-MS Homo sapiens
78 TTC9C 283237
Affinity Capture-MS Homo sapiens
79 RPS16 6217
Affinity Capture-MS Homo sapiens
80 RPL13 6137
Affinity Capture-MS Homo sapiens
81 ARRB2 409
Affinity Capture-MS Homo sapiens
82 PLS1 5357
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 WDR34 89891
Affinity Capture-MS Homo sapiens
84 TERF2  
Affinity Capture-MS Homo sapiens
85 PARP1 142
Cross-Linking-MS (XL-MS) Homo sapiens
86 DSTYK 25778
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 APEX1 328
Affinity Capture-RNA Homo sapiens
88 KEAP1 9817
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 CDC37 11140
Affinity Capture-MS Homo sapiens
90 IKBKG 8517
Affinity Capture-MS Homo sapiens
91 TUBB3 10381
Affinity Capture-MS Homo sapiens
92 CDK2 1017
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 H2AFB3  
Affinity Capture-MS Homo sapiens
95 PALD1 27143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 HIST1H1B 3009
Affinity Capture-MS Homo sapiens
97 MKI67  
Affinity Capture-MS Homo sapiens
98 TUBB4A 10382
Affinity Capture-MS Homo sapiens
99 CDK1 983
Affinity Capture-MS Homo sapiens
100 KRR1 11103
Affinity Capture-MS Homo sapiens
101 LARP7 51574
Affinity Capture-MS Homo sapiens
102 TERF1 7013
Affinity Capture-MS Homo sapiens
103 CDK4 1019
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 CDK9 1025
Affinity Capture-MS Homo sapiens
105 Lck 16818
Affinity Capture-MS Mus musculus
106 C1QBP 708
Proximity Label-MS Homo sapiens
107 MTA3 57504
Co-fractionation Homo sapiens
108 RPL23A 6147
Affinity Capture-MS Homo sapiens
109 NSUN5  
Co-fractionation Homo sapiens
110 EDRF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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