Gene description for RNASEH1
Gene name ribonuclease H1
Gene symbol RNASEH1
Other names/aliases H1RNA
RNH1
Species Homo sapiens
 Database cross references - RNASEH1
ExoCarta ExoCarta_246243
Vesiclepedia VP_246243
Entrez Gene 246243
HGNC 18466
MIM 604123
UniProt O60930  
 RNASEH1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for RNASEH1
Molecular Function
    magnesium ion binding GO:0000287 IEA
    nucleic acid binding GO:0003676 TAS
    RNA binding GO:0003723 TAS
    RNA-DNA hybrid ribonuclease activity GO:0004523 IBA
    RNA-DNA hybrid ribonuclease activity GO:0004523 IDA
    RNA nuclease activity GO:0004540 TAS
    protein binding GO:0005515 IPI
Biological Process
    RNA catabolic process GO:0006401 TAS
    DNA replication, removal of RNA primer GO:0043137 IBA
Subcellular Localization
    mitochondrion GO:0005739 HTP
 Experiment description of studies that identified RNASEH1 in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for RNASEH1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 CACTIN  
Affinity Capture-MS Homo sapiens
3 DDX50 79009
Affinity Capture-MS Homo sapiens
4 SNTG1  
Affinity Capture-MS Homo sapiens
5 RBM27  
Affinity Capture-MS Homo sapiens
6 CLEC3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 TM9SF1 10548
Two-hybrid Homo sapiens
8 RPA2 6118
Affinity Capture-MS Homo sapiens
9 FOXD4  
Affinity Capture-MS Homo sapiens
10 RPA1 6117
Affinity Capture-MS Homo sapiens
11 FAM3C 10447
Two-hybrid Homo sapiens
12 CMAS 55907
Affinity Capture-MS Homo sapiens
13 ZNF638 27332
Affinity Capture-MS Homo sapiens
14 EMD 2010
Two-hybrid Homo sapiens
15 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
16 NRM 11270
Two-hybrid Homo sapiens
17 RBM23  
Affinity Capture-MS Homo sapiens
18 TMEM11 8834
Two-hybrid Homo sapiens
19 UACA 55075
Affinity Capture-MS Homo sapiens
20 MAGED4B  
Affinity Capture-MS Homo sapiens
21 SERPINF2 5345
Affinity Capture-MS Homo sapiens
22 RBM26 64062
Affinity Capture-MS Homo sapiens
23 CDK13 8621
Affinity Capture-MS Homo sapiens
24 RPA3 6119
Affinity Capture-MS Homo sapiens
25 HIBADH 11112
Affinity Capture-MS Homo sapiens
26 CHCHD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 ASPH 444
Two-hybrid Homo sapiens
28 C1QBP 708
Affinity Capture-MS Homo sapiens
29 NCS1 23413
Two-hybrid Homo sapiens
30 DDX54 79039
Affinity Capture-MS Homo sapiens
31 BHLHA15  
Affinity Capture-MS Homo sapiens
32 CHCHD10  
Affinity Capture-MS Homo sapiens
33 ECM1 1893
Affinity Capture-MS Homo sapiens
34 PHF10  
Affinity Capture-MS Homo sapiens
35 NKTR  
Affinity Capture-MS Homo sapiens
36 SCAF11  
Affinity Capture-MS Homo sapiens
37 LUC7L 55692
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 MZF1  
Affinity Capture-MS Homo sapiens
39 TRIM63  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which RNASEH1 is involved
No pathways found





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