Gene description for RNASEH1
Gene name ribonuclease H1
Gene symbol RNASEH1
Other names/aliases H1RNA
RNH1
Species Homo sapiens
 Database cross references - RNASEH1
ExoCarta ExoCarta_246243
Entrez Gene 246243
HGNC 18466
MIM 604123
UniProt O60930  
 RNASEH1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for RNASEH1
Molecular Function
    RNA binding GO:0003723 TAS
    magnesium ion binding GO:0000287 IEA
    ribonuclease activity GO:0004540 TAS
    RNA-DNA hybrid ribonuclease activity GO:0004523 IDA
    nucleic acid binding GO:0003676 TAS
    protein homodimerization activity GO:0042803 IEA
Biological Process
    RNA catabolic process GO:0006401 TAS
    RNA phosphodiester bond hydrolysis GO:0090501 TAS
    mitochondrial DNA replication GO:0006264 IEA
    DNA replication, removal of RNA primer GO:0043137 IBA
    RNA phosphodiester bond hydrolysis, endonucleolytic GO:0090502 IEA
Subcellular Localization
    mitochondrion GO:0005739 IEA
    nucleus GO:0005634 IEA
 Experiment description of studies that identified RNASEH1 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for RNASEH1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which RNASEH1 is involved
No pathways found





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