Gene description for DDX54
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
Gene symbol DDX54
Other names/aliases DP97
Species Homo sapiens
 Database cross references - DDX54
ExoCarta ExoCarta_79039
Entrez Gene 79039
HGNC 20084
MIM 611665
UniProt Q8TDD1  
 DDX54 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for DDX54
Molecular Function
    transcription corepressor activity GO:0003714 IDA
    ATP binding GO:0005524 IEA
    ATP-dependent RNA helicase activity GO:0004004 IDA
    estrogen receptor binding GO:0030331 IDA
    receptor binding GO:0005102 IDA
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    regulation of transcription, DNA-templated GO:0006355 IEA
    transcription, DNA-templated GO:0006351 IEA
    RNA processing GO:0006396 IDA
    negative regulation of nucleic acid-templated transcription GO:1903507 IDA
    intracellular estrogen receptor signaling pathway GO:0030520 TAS
    RNA secondary structure unwinding GO:0010501 IBA
    RNA metabolic process GO:0016070 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    membrane GO:0016020 IDA
    nucleolus GO:0005730 IDA
 Experiment description of studies that identified DDX54 in exosomes
1
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for DDX54
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ESR2  
Invitro Homo sapiens
2 ESR1  
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 PGR  
Invitro Homo sapiens
4 NR3C1 2908
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which DDX54 is involved
No pathways found





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