Gene description for DDX54
Gene name
DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
Gene symbol
DDX54
Other names/aliases
DP97
Species
Homo sapiens
Database cross references - DDX54
ExoCarta
ExoCarta_79039
Entrez Gene
79039
HGNC
20084
MIM
611665
UniProt
Q8TDD1
DDX54 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells
26054723
Hepatocytes
26054723
Gene ontology annotations for DDX54
Experiment description of studies that identified DDX54 in exosomes
1
Experiment ID
236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
MHCC97L
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
2
Experiment ID
237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocytes
Sample name
MIHA
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RNA Sequencing
Protein-protein interactions for DDX54
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
ESR2
Invitro
Homo sapiens
2
ESR1
Invitro
Homo sapiens
Two-hybrid
Homo sapiens
3
PGR
Invitro
Homo sapiens
4
NR3C1
2908
Invitro
Homo sapiens
View the network
image/svg+xml
Pathways in which DDX54 is involved
No pathways found