Gene description for DCK
Gene name deoxycytidine kinase
Gene symbol DCK
Other names/aliases -
Species Homo sapiens
 Database cross references - DCK
ExoCarta ExoCarta_1633
Vesiclepedia VP_1633
Entrez Gene 1633
HGNC 2704
MIM 125450
UniProt P27707  
 DCK identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for DCK
Molecular Function
    deoxyadenosine kinase activity GO:0004136 IDA
    deoxycytidine kinase activity GO:0004137 IBA
    deoxycytidine kinase activity GO:0004137 IDA
    deoxyguanosine kinase activity GO:0004138 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein homodimerization activity GO:0042803 IPI
    cytidine kinase activity GO:0043771 IDA
Biological Process
    pyrimidine nucleotide metabolic process GO:0006220 IDA
    CMP biosynthetic process GO:0009224 IEA
    dAMP salvage GO:0106383 IEA
    nucleoside phosphate biosynthetic process GO:1901293 IDA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified DCK in exosomes
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DCK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TFEC  
Two-hybrid Homo sapiens
2 PRTN3 5657
Affinity Capture-MS Homo sapiens
3 MED19  
Two-hybrid Homo sapiens
4 ISX  
Affinity Capture-MS Homo sapiens
5 CHRAC1  
Co-fractionation Homo sapiens
6 ADSS 159
Co-fractionation Homo sapiens
7 IDH1 3417
Co-fractionation Homo sapiens
8 DCK 1633
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
9 MSH6 2956
Co-fractionation Homo sapiens
10 TPK1  
Affinity Capture-MS Homo sapiens
11 TYMS 7298
Co-fractionation Homo sapiens
12 HSPE1 3336
Co-fractionation Homo sapiens
13 DDX19B 11269
Two-hybrid Homo sapiens
14 TALDO1 6888
Co-fractionation Homo sapiens
15 RBBP7 5931
Co-fractionation Homo sapiens
16 DACH1  
Affinity Capture-MS Homo sapiens
17 CBX3 11335
Co-fractionation Homo sapiens
18 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
19 PLEKHG2 64857
Affinity Capture-MS Homo sapiens
20 IRF2BPL 64207
Affinity Capture-MS Homo sapiens
21 P2RY8  
Affinity Capture-MS Homo sapiens
22 GZMH  
Affinity Capture-MS Homo sapiens
23 EFHD2 79180
Co-fractionation Homo sapiens
24 NUTM2F  
Affinity Capture-MS Homo sapiens
25 KLK3  
Affinity Capture-MS Homo sapiens
26 NTRK1 4914
Affinity Capture-MS Homo sapiens
27 GPR45  
Affinity Capture-MS Homo sapiens
28 MBL2 4153
Affinity Capture-MS Homo sapiens
29 GPR182  
Affinity Capture-MS Homo sapiens
30 DGUOK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 RXRG  
Two-hybrid Homo sapiens
32 ZNF503 84858
Affinity Capture-MS Homo sapiens
33 PTMS 5763
Co-fractionation Homo sapiens
34 RANBP1 5902
Co-fractionation Homo sapiens
35 GOLM1 51280
Two-hybrid Homo sapiens
36 NUCKS1 64710
Co-fractionation Homo sapiens
37 ANKRD13A 88455
Affinity Capture-MS Homo sapiens
38 THOP1 7064
Co-fractionation Homo sapiens
39 CHMP4A 29082
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which DCK is involved
PathwayEvidenceSource
Metabolism TAS Reactome
Metabolism of nucleotides TAS Reactome
Nucleotide salvage TAS Reactome
Purine salvage TAS Reactome
Pyrimidine salvage TAS Reactome





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