Gene description for OAS3
Gene name 2'-5'-oligoadenylate synthetase 3, 100kDa
Gene symbol OAS3
Other names/aliases p100
p100OAS
Species Homo sapiens
 Database cross references - OAS3
ExoCarta ExoCarta_4940
Vesiclepedia VP_4940
Entrez Gene 4940
HGNC 8088
MIM 603351
UniProt Q9Y6K5  
 OAS3 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Endothelial cells 26027894    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for OAS3
Molecular Function
    2'-5'-oligoadenylate synthetase activity GO:0001730 IBA
    2'-5'-oligoadenylate synthetase activity GO:0001730 IDA
    double-stranded RNA binding GO:0003725 IBA
    double-stranded RNA binding GO:0003725 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    nucleobase-containing compound metabolic process GO:0006139 TAS
    response to virus GO:0009615 IDA
    positive regulation of interferon-beta production GO:0032728 IMP
    positive regulation of tumor necrosis factor production GO:0032760 IMP
    negative regulation of chemokine (C-X-C motif) ligand 9 production GO:0035395 IMP
    RIG-I signaling pathway GO:0039529 IMP
    MDA-5 signaling pathway GO:0039530 IMP
    defense response to bacterium GO:0042742 IMP
    negative regulation of viral genome replication GO:0045071 IBA
    negative regulation of viral genome replication GO:0045071 IDA
    defense response to virus GO:0051607 IDA
    type I interferon-mediated signaling pathway GO:0060337 IBA
    negative regulation of type I interferon-mediated signaling pathway GO:0060339 IMP
    regulation of ribonuclease activity GO:0060700 IDA
    interleukin-27-mediated signaling pathway GO:0070106 IBA
    positive regulation of monocyte chemotactic protein-1 production GO:0071639 IMP
    negative regulation of chemokine (C-C motif) ligand 5 production GO:0071650 IMP
    negative regulation of IP-10 production GO:0071659 IMP
    antiviral innate immune response GO:0140374 IBA
    negative regulation of chemokine (C-X-C motif) ligand 2 production GO:2000342 IMP
Subcellular Localization
    extracellular space GO:0005615 HDA
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 IBA
    intracellular membrane-bounded organelle GO:0043231 TAS
 Experiment description of studies that identified OAS3 in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
3
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for OAS3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAVS 57506
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
2 PABPC4 8761
Affinity Capture-MS Homo sapiens
3 EIF6 3692
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
4 E2F4  
Affinity Capture-MS Homo sapiens
5 ZNF622 90441
Affinity Capture-MS Homo sapiens
6 CHMP1A 5119
Co-fractionation Homo sapiens
7 TOP1 7150
Affinity Capture-MS Homo sapiens
8 PARK2  
Affinity Capture-MS Homo sapiens
9 DHX30 22907
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
10 MKI67  
Affinity Capture-MS Homo sapiens
11 PPM1G 5496
Affinity Capture-MS Homo sapiens
12 IFRD2 7866
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
13 SP110  
Affinity Capture-MS Homo sapiens
14 NTRK1 4914
Affinity Capture-MS Homo sapiens
15 CIT 11113
Affinity Capture-MS Homo sapiens
16 FBXW7  
Affinity Capture-MS Homo sapiens
17 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
18 LYAR 55646
Affinity Capture-MS Homo sapiens
19 CCDC22 28952
Affinity Capture-MS Homo sapiens
20 BRPF3  
Affinity Capture-MS Homo sapiens
21 HSPA12A 259217
Co-fractionation Homo sapiens
22 PML 5371
Affinity Capture-MS Homo sapiens
23 FTSJ3 117246
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
24 Eea1  
Affinity Capture-MS Mus musculus
25 GTPBP4 23560
Affinity Capture-MS Homo sapiens
26 GNL3 26354
Affinity Capture-MS Homo sapiens
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