Gene description for OAS3
Gene name 2'-5'-oligoadenylate synthetase 3, 100kDa
Gene symbol OAS3
Other names/aliases p100
p100OAS
Species Homo sapiens
 Database cross references - OAS3
ExoCarta ExoCarta_4940
Entrez Gene 4940
HGNC 8088
MIM 603351
UniProt Q9Y6K5  
 OAS3 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Thymus 23844026    
 Gene ontology annotations for OAS3
Molecular Function
    ATP binding GO:0005524 IDA
    metal ion binding GO:0046872 IEA
    2'-5'-oligoadenylate synthetase activity GO:0001730 IDA
    double-stranded RNA binding GO:0003725 IDA
    protein binding GO:0005515 IPI
Biological Process
    nucleobase-containing compound metabolic process GO:0006139 TAS
    cytokine-mediated signaling pathway GO:0019221 TAS
    response to virus GO:0009615 IDA
    negative regulation of viral genome replication GO:0045071 IDA
    interferon-gamma-mediated signaling pathway GO:0060333 TAS
    type I interferon signaling pathway GO:0060337 TAS
    defense response to virus GO:0051607 IDA
    regulation of ribonuclease activity GO:0060700 IDA
Subcellular Localization
    extracellular space GO:0005615 IDA
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    intracellular membrane-bounded organelle GO:0043231 TAS
    nucleoplasm GO:0005654 IDA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified OAS3 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for OAS3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which OAS3 is involved
PathwayEvidenceSource
Interferon alpha/beta signaling TAS Reactome
Interferon gamma signaling TAS Reactome





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