| Experiment ID |
236 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|Alix|HSC70|GAPDH
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✔
qNano
|
Particle analysis
|
|
| Identified molecule |
mRNA
|
| Identification method |
RNA Sequencing
|
| PubMed ID |
26054723
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
| Authors |
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" |
| Journal name |
Carcinogenesis
|
| Publication year |
2015 |
| Sample |
Hepatocellular carcinoma cells |
| Sample name |
MHCC97L |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein
RNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing |
Gene description for TULP4