Gene description for ADAM17
Gene name ADAM metallopeptidase domain 17
Gene symbol ADAM17
Other names/aliases ADAM18
CD156B
CSVP
NISBD
NISBD1
TACE
Species Homo sapiens
 Database cross references - ADAM17
ExoCarta ExoCarta_6868
Entrez Gene 6868
HGNC 195
MIM 603639
UniProt P78536  
 ADAM17 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for ADAM17
Molecular Function
    PDZ domain binding GO:0030165 IPI
    metalloendopeptidase activity GO:0004222 TAS
    Notch binding GO:0005112 IDA
    metallopeptidase activity GO:0008237 TAS
    integrin binding GO:0005178 IPI
    interleukin-6 receptor binding GO:0005138 IPI
    SH3 domain binding GO:0017124 IEA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
Biological Process
    T cell differentiation in thymus GO:0033077 ISS
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IMP
    spleen development GO:0048536 ISS
    positive regulation of cellular component movement GO:0051272 ISS
    cell adhesion mediated by integrin GO:0033627 IDA
    response to high density lipoprotein particle GO:0055099 IDA
    tumor necrosis factor-mediated signaling pathway GO:0033209 TAS
    extracellular matrix organization GO:0030198 TAS
    proteolysis GO:0006508 IDA
    cell adhesion GO:0007155 IDA
    positive regulation of epidermal growth factor-activated receptor activity GO:0045741 IMP
    positive regulation of chemokine production GO:0032722 IMP
    positive regulation of protein phosphorylation GO:0001934 IMP
    response to hypoxia GO:0001666 IDA
    JAK-STAT cascade involved in growth hormone signaling pathway GO:0060397 TAS
    Notch receptor processing GO:0007220 IDA
    wound healing, spreading of epidermal cells GO:0035313 IEP
    positive regulation of cell migration GO:0030335 IMP
    regulation of mast cell apoptotic process GO:0033025 ISS
    membrane protein intracellular domain proteolysis GO:0031293 TAS
    defense response to Gram-positive bacterium GO:0050830 IMP
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    positive regulation of cell proliferation GO:0008284 IMP
    response to drug GO:0042493 ISS
    germinal center formation GO:0002467 ISS
    epidermal growth factor-activated receptor transactivation by G-protein coupled receptor signaling pathway GO:0035625 IMP
    collagen catabolic process GO:0030574 TAS
    positive regulation of transforming growth factor beta receptor signaling pathway GO:0030511 ISS
    positive regulation of leukocyte chemotaxis GO:0002690 IC
    positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle GO:0031659 IDA
    membrane protein ectodomain proteolysis GO:0006509 IMP
    cell surface receptor signaling pathway GO:0007166 TAS
    PMA-inducible membrane protein ectodomain proteolysis GO:0051088 IMP
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    positive regulation of T cell chemotaxis GO:0010820 IMP
    extracellular matrix disassembly GO:0022617 TAS
    B cell differentiation GO:0030183 ISS
    cell motility GO:0048870 ISS
    neutrophil mediated immunity GO:0002446 IC
    positive regulation of cell growth GO:0030307 IMP
    response to lipopolysaccharide GO:0032496 IDA
    Notch signaling pathway GO:0007219 IEA
Subcellular Localization
    cell surface GO:0009986 IDA
    actin cytoskeleton GO:0015629 IDA
    plasma membrane GO:0005886 TAS
    membrane raft GO:0045121 IDA
    cytoplasm GO:0005737 IDA
    membrane GO:0016020 IDA
    apical plasma membrane GO:0016324 IDA
    focal adhesion GO:0005925 IDA
    ruffle membrane GO:0032587 IDA
    integral component of plasma membrane GO:0005887 IDA
    cell-cell junction GO:0005911 IDA
 Experiment description of studies that identified ADAM17 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ADAM17
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DLG1 1739
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
2 MAPK1 5594
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 TIMP3 7078
Reconstituted Complex Homo sapiens
4 MAD2L1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here