Gene description for CDCA8
Gene name cell division cycle associated 8
Gene symbol CDCA8
Other names/aliases BOR
BOREALIN
DasraB
MESRGP
Species Homo sapiens
 Database cross references - CDCA8
ExoCarta ExoCarta_55143
Vesiclepedia VP_55143
Entrez Gene 55143
HGNC 14629
MIM 609977
UniProt Q53HL2  
 CDCA8 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Melanoma cells 25950383    
 Gene ontology annotations for CDCA8
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    mitotic sister chromatid segregation GO:0000070 IBA
    mitotic cell cycle GO:0000278 NAS
    mitotic cytokinesis GO:0000281 NAS
    mitotic metaphase chromosome alignment GO:0007080 IMP
    mitotic spindle midzone assembly GO:0051256 NAS
    chromosome organization GO:0051276 IMP
    positive regulation of mitotic cell cycle spindle assembly checkpoint GO:0090267 NAS
    mitotic spindle assembly GO:0090307 NAS
    positive regulation of mitotic sister chromatid separation GO:1901970 NAS
    positive regulation of attachment of mitotic spindle microtubules to kinetochore GO:1902425 NAS
    positive regulation of mitotic cytokinesis GO:1903490 NAS
Subcellular Localization
    chromosome, centromeric region GO:0000775 IBA
    chromosome, centromeric region GO:0000775 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 TAS
    chromocenter GO:0010369 IEA
    microtubule cytoskeleton GO:0015630 IDA
    midbody GO:0030496 IDA
    chromosome passenger complex GO:0032133 IBA
    chromosome passenger complex GO:0032133 IPI
    protein-containing complex GO:0032991 IDA
    intercellular bridge GO:0045171 IDA
    spindle midzone GO:0051233 IBA
 Experiment description of studies that identified CDCA8 in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
5
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CDCA8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDC14B 8555
Proximity Label-MS Homo sapiens
2 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 SENP3 26168
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
5 KIF23 9493
Affinity Capture-MS Homo sapiens
6 LILRA5  
Affinity Capture-MS Homo sapiens
7 MTHFR 4524
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 EEF1G 1937
Affinity Capture-MS Homo sapiens
9 VSNL1 7447
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 NOP9 161424
Affinity Capture-MS Homo sapiens
11 Nfyc 18046
Affinity Capture-MS Mus musculus
12 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
13 GYS1 2997
Two-hybrid Homo sapiens
14 KIF20A 10112
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 INCENP 3619
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
16 CENPA  
Proximity Label-MS Homo sapiens
17 APEX1 328
Proximity Label-MS Homo sapiens
18 Rrp1b 72462
Affinity Capture-MS Mus musculus
19 Aurkb  
Affinity Capture-MS Mus musculus
20 EMILIN1 11117
Two-hybrid Homo sapiens
21 HLA-DRB3 3125
Two-hybrid Homo sapiens
22 FBXO17 115290
Two-hybrid Homo sapiens
23 DPYSL2 1808
Affinity Capture-MS Homo sapiens
24 VARS 7407
Affinity Capture-MS Homo sapiens
25 CHCHD2  
Affinity Capture-MS Homo sapiens
26 PRC1 9055
Affinity Capture-MS Homo sapiens
27 OBSL1 23363
Affinity Capture-MS Homo sapiens
28 NEK3  
Two-hybrid Homo sapiens
29 CRMP1 1400
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 KIF2C 11004
Affinity Capture-MS Homo sapiens
31 CDCA8 55143
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
32 ASF1A 25842
Proximity Label-MS Homo sapiens
33 TNFRSF1B  
Affinity Capture-MS Homo sapiens
34 SGOL2 151246
Two-hybrid Homo sapiens
35 EEF1A1 1915
Affinity Capture-MS Homo sapiens
36 KIF14 9928
Affinity Capture-MS Homo sapiens
37 CHMP4C 92421
Affinity Capture-MS Homo sapiens
38 XPO1 7514
Affinity Capture-Western Homo sapiens
39 CAMK2A 815
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 ZNF277  
Two-hybrid Homo sapiens
41 S100A2 6273
Affinity Capture-MS Homo sapiens
42 CUL4B 8450
Affinity Capture-MS Homo sapiens
43 SNAP91 9892
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 LYN 4067
Proximity Label-MS Homo sapiens
45 RCC2 55920
Affinity Capture-Western Homo sapiens
46 CBX3 11335
Proximity Label-MS Homo sapiens
47 UBE2I 7329
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
48 MAPT  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 CHMP4B 128866
Affinity Capture-MS Homo sapiens
50 MYH9 4627
Affinity Capture-MS Homo sapiens
51 WDYHV1  
Two-hybrid Homo sapiens
52 CBX5 23468
Affinity Capture-MS Homo sapiens
53 PIAS2  
Two-hybrid Homo sapiens
54 Cdca8  
Affinity Capture-MS Mus musculus
55 MB21D1  
Proximity Label-MS Homo sapiens
56 ATP4A 495
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 HECTD1 25831
Affinity Capture-MS Homo sapiens
58 SPANXN2  
Two-hybrid Homo sapiens
59 MYH3 4621
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 MYH4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 CD3EAP  
Proximity Label-MS Homo sapiens
62 ANLN 54443
Affinity Capture-MS Homo sapiens
63 Incenp  
Affinity Capture-MS Mus musculus
64 ZBTB2 57621
Affinity Capture-MS Homo sapiens
65 S100P 6286
Affinity Capture-MS Homo sapiens
66 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
67 SNAPC5  
Two-hybrid Homo sapiens
68 HIST1H3A 8350
Proximity Label-MS Homo sapiens
69 DCLK1 9201
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 C11orf57  
Two-hybrid Homo sapiens
71 HIST2H3C 126961
Reconstituted Complex Homo sapiens
72 TK2  
Affinity Capture-MS Homo sapiens
73 ECT2 1894
Affinity Capture-MS Homo sapiens
74 STXBP1 6812
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 SGOL1  
Two-hybrid Homo sapiens
76 DCAF13 25879
Affinity Capture-MS Homo sapiens
77 HMBOX1  
Two-hybrid Homo sapiens
78 EEF1B2 1933
Affinity Capture-MS Homo sapiens
79 GPM6B 2824
Affinity Capture-MS Homo sapiens
80 GNG2 54331
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 SYP  
Affinity Capture-MS Homo sapiens
82 TRIM44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 SET 6418
Two-hybrid Homo sapiens
84 MAPKAPK5 8550
Two-hybrid Homo sapiens
85 MYH7 4625
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 DPYSL3 1809
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 BIRC5  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
89 PARP1 142
Proximity Label-MS Homo sapiens
90 USP35  
Affinity Capture-Western Homo sapiens
91 AURKB 9212
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
92 PIH1D2  
Two-hybrid Homo sapiens
93 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
94 RPS24 6229
Proximity Label-MS Homo sapiens
95 EEF1D 1936
Affinity Capture-MS Homo sapiens
96 NEDD1 121441
Affinity Capture-MS Homo sapiens
97 RPS6 6194
Proximity Label-MS Homo sapiens
98 RPRD1B 58490
Two-hybrid Homo sapiens
99 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
100 NUCKS1 64710
Affinity Capture-MS Homo sapiens
101 RANBP2 5903
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
102 SYT1 6857
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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