Gene description for MYH3
Gene name myosin, heavy chain 3, skeletal muscle, embryonic
Gene symbol MYH3
Other names/aliases HEMHC
MYHC-EMB
MYHSE1
SMHCE
Species Homo sapiens
 Database cross references - MYH3
ExoCarta ExoCarta_4621
Vesiclepedia VP_4621
Entrez Gene 4621
HGNC 7573
MIM 160720
UniProt P11055  
 MYH3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Urine 19056867    
 Gene ontology annotations for MYH3
Molecular Function
    microfilament motor activity GO:0000146 IBA
    microfilament motor activity GO:0000146 IMP
    microfilament motor activity GO:0000146 NAS
    calmodulin binding GO:0005516 NAS
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IMP
    myosin phosphatase activity GO:0017018 TAS
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IMP
Biological Process
    skeletal muscle contraction GO:0003009 IMP
    muscle contraction GO:0006936 IBA
    muscle organ development GO:0007517 TAS
    actin filament-based movement GO:0030048 NAS
    muscle filament sliding GO:0030049 TAS
    embryonic limb morphogenesis GO:0030326 IC
    sarcomere organization GO:0045214 NAS
    ATP metabolic process GO:0046034 IMP
    face morphogenesis GO:0060325 IC
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    muscle myosin complex GO:0005859 NAS
    myosin II complex GO:0016460 IBA
    sarcomere GO:0030017 NAS
    myosin filament GO:0032982 IBA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified MYH3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for MYH3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANLN 54443
Affinity Capture-MS Homo sapiens
2 CDCA8 55143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CUL1 8454
Affinity Capture-MS Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 C5orf24 134553
Affinity Capture-MS Homo sapiens
6 TPM2 7169
Co-fractionation Homo sapiens
7 EFNA1  
Affinity Capture-MS Homo sapiens
8 HIST1H4A 8359
Cross-Linking-MS (XL-MS) Homo sapiens
9 TLE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 ABTB2 25841
Affinity Capture-MS Homo sapiens
11 SIX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 MKI67  
Affinity Capture-MS Homo sapiens
13 LATS1  
Affinity Capture-MS Homo sapiens
14 ASAH2 56624
Affinity Capture-MS Homo sapiens
15 ZNRF2 223082
Affinity Capture-MS Homo sapiens
16 TMEM260  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 AKAP17A  
Affinity Capture-MS Homo sapiens
18 MYH6 4624
Affinity Capture-MS Homo sapiens
19 ACTN2 88
Co-fractionation Homo sapiens
20 KRT40  
Affinity Capture-MS Homo sapiens
21 MYH4  
Affinity Capture-MS Homo sapiens
22 TAF6L 10629
Affinity Capture-MS Homo sapiens
23 TNNI1 7135
Co-fractionation Homo sapiens
24 HIF1A 3091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MYH3 is involved
PathwayEvidenceSource
Muscle contraction TAS Reactome
Striated Muscle Contraction TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here