Gene description for ZNRF2
Gene name zinc and ring finger 2, E3 ubiquitin protein ligase
Gene symbol ZNRF2
Other names/aliases RNF202
Species Homo sapiens
 Database cross references - ZNRF2
ExoCarta ExoCarta_223082
Vesiclepedia VP_223082
Entrez Gene 223082
HGNC 22316
MIM 612061
UniProt Q8NHG8  
 ZNRF2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ZNRF2
Molecular Function
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 IDA
Biological Process
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IC
    protein K48-linked ubiquitination GO:0070936 IBA
    positive regulation of autophagosome-lysosome fusion GO:0160177 IGI
Subcellular Localization
    fibrillar center GO:0001650 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    lysosomal membrane GO:0005765 IEA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    endosome membrane GO:0010008 IEA
    membrane GO:0016020 IBA
    cytoplasmic vesicle membrane GO:0030659 IDA
    protein-containing complex GO:0032991 IDA
    presynaptic membrane GO:0042734 IEA
    cell projection GO:0042995 IEA
    intracellular membrane-bounded organelle GO:0043231 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified ZNRF2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ZNRF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATP1A2 477
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
2 PPP6R1 22870
Affinity Capture-MS Homo sapiens
3 UBE2N 7334
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
4 ARHGEF2 9181
Affinity Capture-MS Homo sapiens
5 CGB1  
Affinity Capture-MS Homo sapiens
6 LAMTOR1 55004
Affinity Capture-Western Homo sapiens
7 EEA1 8411
Affinity Capture-MS Homo sapiens
8 XPO1 7514
Affinity Capture-MS Homo sapiens
9 ATP1A1 476
Biochemical Activity Homo sapiens
10 ATP6V0A2 23545
Affinity Capture-Western Homo sapiens
11 RPTOR 57521
Affinity Capture-Western Homo sapiens
12 ZNRF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
13 UBC 7316
Biochemical Activity Homo sapiens
14 UBE2E1  
Two-hybrid Homo sapiens
15 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
16 UBE2D4  
Two-hybrid Homo sapiens
17 PPP6R2 9701
Affinity Capture-MS Homo sapiens
18 PPP6R3 55291
Affinity Capture-Western Homo sapiens
19 ANKRD28 23243
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
20 NCKAP5  
Affinity Capture-MS Homo sapiens
21 CHCHD4  
Affinity Capture-MS Homo sapiens
22 UBE2D2 7322
Two-hybrid Homo sapiens
23 ANKRD52 283373
Affinity Capture-MS Homo sapiens
24 PPP6C 5537
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
25 MTOR 2475
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
26 TLN1 7094
Affinity Capture-MS Homo sapiens
27 KLHDC10 23008
Affinity Capture-MS Homo sapiens
28 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
29 ABCA2 20
Affinity Capture-MS Homo sapiens
30 UBE2D3 7323
Two-hybrid Homo sapiens
31 MLST8 64223
Affinity Capture-Western Homo sapiens
32 MYH3 4621
Affinity Capture-MS Homo sapiens
33 SLC37A4 2542
Affinity Capture-MS Homo sapiens
34 ANKRD44 91526
Affinity Capture-MS Homo sapiens
35 CCDC8  
Affinity Capture-MS Homo sapiens
36 WDR5B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 UBE2D1 7321
Two-hybrid Homo sapiens
View the network image/svg+xml



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