Gene description for MYH6
Gene name myosin, heavy chain 6, cardiac muscle, alpha
Gene symbol MYH6
Other names/aliases ASD3
CMD1EE
CMH14
MYHC
MYHCA
SSS3
alpha-MHC
Species Homo sapiens
 Database cross references - MYH6
ExoCarta ExoCarta_4624
Vesiclepedia VP_4624
Entrez Gene 4624
HGNC 7576
MIM 160710
UniProt P13533  
 MYH6 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MYH6
Molecular Function
    microfilament motor activity GO:0000146 IBA
    microfilament motor activity GO:0000146 IDA
    microfilament motor activity GO:0000146 IMP
    microfilament motor activity GO:0000146 ISS
    calmodulin binding GO:0005516 IEA
    ATP binding GO:0005524 IEA
    myosin phosphatase activity GO:0017018 TAS
    protein kinase binding GO:0019901 IPI
    actin filament binding GO:0051015 IBA
Biological Process
    in utero embryonic development GO:0001701 ISS
    regulation of the force of heart contraction GO:0002026 IDA
    regulation of the force of heart contraction GO:0002026 ISS
    regulation of heart rate GO:0002027 IDA
    muscle contraction GO:0006936 IDA
    striated muscle contraction GO:0006941 IMP
    striated muscle contraction GO:0006941 ISS
    adult heart development GO:0007512 IMP
    visceral muscle development GO:0007522 ISS
    regulation of heart contraction GO:0008016 ISS
    regulation of blood pressure GO:0008217 ISS
    cardiac muscle hypertrophy in response to stress GO:0014898 IEA
    muscle filament sliding GO:0030049 IMP
    muscle filament sliding GO:0030049 TAS
    myofibril assembly GO:0030239 ISS
    sarcomere organization GO:0045214 ISS
    ATP metabolic process GO:0046034 IDA
    atrial cardiac muscle tissue morphogenesis GO:0055009 IMP
    ventricular cardiac muscle tissue morphogenesis GO:0055010 IMP
    cardiac muscle cell development GO:0055013 ISS
    cardiac muscle contraction GO:0060048 ISS
    regulation of heart growth GO:0060420 IEA
Subcellular Localization
    stress fiber GO:0001725 IEA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    muscle myosin complex GO:0005859 TAS
    myosin complex GO:0016459 TAS
    myosin II complex GO:0016460 IBA
    myofibril GO:0030016 ISS
    sarcomere GO:0030017 TAS
    Z disc GO:0030018 IEA
    myosin filament GO:0032982 IBA
 Experiment description of studies that identified MYH6 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MYH6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BAG3 9531
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 SPICE1  
Affinity Capture-MS Homo sapiens
3 HSPB2  
Two-hybrid Homo sapiens
4 TCEB1 6921
Affinity Capture-MS Homo sapiens
5 NSF 4905
Affinity Capture-MS Homo sapiens
6 GJA1 2697
Affinity Capture-MS Homo sapiens
7 TPM2 7169
Co-fractionation Homo sapiens
8 VPRBP 9730
Affinity Capture-MS Homo sapiens
9 FOXA1  
Affinity Capture-MS Homo sapiens
10 COPE 11316
Affinity Capture-MS Homo sapiens
11 MSX2  
Affinity Capture-MS Homo sapiens
12 TRIP11 9321
Affinity Capture-MS Homo sapiens
13 DOCK6 57572
Affinity Capture-MS Homo sapiens
14 OPTN 10133
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 LATS1  
Affinity Capture-MS Homo sapiens
16 BDP1  
Affinity Capture-MS Homo sapiens
17 MAP2K3 5606
Affinity Capture-MS Homo sapiens
18 TPM1 7168
Co-fractionation Homo sapiens
19 ABTB2 25841
Affinity Capture-MS Homo sapiens
20 UMPS 7372
Affinity Capture-MS Homo sapiens
21 TMEM132A 54972
Affinity Capture-MS Homo sapiens
22 ITPRIPL2 162073
Affinity Capture-MS Homo sapiens
23 DYRK1A 1859
Affinity Capture-MS Homo sapiens
24 HMGN1  
Affinity Capture-MS Homo sapiens
25 FBXO31 79791
Affinity Capture-MS Homo sapiens
26 MCM2 4171
Affinity Capture-MS Homo sapiens
27 SORT1 6272
Affinity Capture-MS Homo sapiens
28 USP24 23358
Affinity Capture-MS Homo sapiens
29 BRD1 23774
Affinity Capture-MS Homo sapiens
30 ACTN2 88
Co-fractionation Homo sapiens
31 TMSB4X 7114
Affinity Capture-MS Homo sapiens
32 Brca1  
Affinity Capture-MS Mus musculus
33 PIGS 94005
Affinity Capture-MS Homo sapiens
34 LZTR1 8216
Affinity Capture-MS Homo sapiens
35 MYH3 4621
Affinity Capture-MS Homo sapiens
36 MCM3AP  
Affinity Capture-MS Homo sapiens
37 SPTY2D1  
Affinity Capture-MS Homo sapiens
38 MYBBP1A 10514
Affinity Capture-MS Homo sapiens
39 MBP 4155
Affinity Capture-MS Homo sapiens
40 TNNI1 7135
Co-fractionation Homo sapiens
41 EDRF1  
Affinity Capture-MS Homo sapiens
42 APOA1 335
Cross-Linking-MS (XL-MS) Homo sapiens
43 HIF1A 3091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MYH6 is involved
PathwayEvidenceSource
Muscle contraction TAS Reactome
Striated Muscle Contraction TAS Reactome





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