Gene description for USP24
Gene name ubiquitin specific peptidase 24
Gene symbol USP24
Other names/aliases -
Species Homo sapiens
 Database cross references - USP24
ExoCarta ExoCarta_23358
Vesiclepedia VP_23358
Entrez Gene 23358
HGNC 12623
MIM 610569
UniProt Q9UPU5  
 USP24 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for USP24
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 TAS
    protein binding GO:0005515 IPI
Biological Process
    proteolysis GO:0006508 IEA
    protein deubiquitination GO:0016579 TAS
    regulation of protein stability GO:0031647 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IBA
 Experiment description of studies that identified USP24 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
EM
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for USP24
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZRANB1 54764
Affinity Capture-MS Homo sapiens
2 GYPA  
Affinity Capture-MS Homo sapiens
3 HNRNPD 3184
Affinity Capture-MS Homo sapiens
4 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 GPR17 2840
Affinity Capture-MS Homo sapiens
7 VIPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 CYB5B 80777
Affinity Capture-MS Homo sapiens
9 SERBP1 26135
Affinity Capture-MS Homo sapiens
10 CXCR4 7852
Affinity Capture-MS Homo sapiens
11 SPSB4  
Affinity Capture-MS Homo sapiens
12 SLC35G1  
Affinity Capture-MS Homo sapiens
13 TP53 7157
Biochemical Activity Homo sapiens
14 IFT20 90410
Affinity Capture-MS Homo sapiens
15 FPR1  
Affinity Capture-MS Homo sapiens
16 HCST  
Affinity Capture-MS Homo sapiens
17 SIRT7  
Affinity Capture-MS Homo sapiens
18 COPS7B 64708
Co-fractionation Homo sapiens
19 RPA2 6118
Proximity Label-MS Homo sapiens
20 NPTN 27020
Affinity Capture-MS Homo sapiens
21 C19orf38  
Affinity Capture-MS Homo sapiens
22 RP2 6102
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 CPNE2 221184
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 IGHM 3507
Affinity Capture-MS Homo sapiens
25 SYMPK 8189
Co-fractionation Homo sapiens
26 CHRM4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 Oxnad1  
Affinity Capture-MS Mus musculus
28 NR3C1 2908
Proximity Label-MS Homo sapiens
29 CNOT2  
Affinity Capture-MS Homo sapiens
30 DDB2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
31 UBC 7316
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
32 CSNK2A1 1457
Biochemical Activity Homo sapiens
33 GPR182  
Affinity Capture-MS Homo sapiens
34 PIBF1  
Affinity Capture-MS Homo sapiens
35 CUL3 8452
Affinity Capture-MS Homo sapiens
36 GPRC5D  
Affinity Capture-MS Homo sapiens
37 TAGLN 6876
Affinity Capture-MS Homo sapiens
38 ZEB1  
Affinity Capture-MS Homo sapiens
39 HTT 3064
Co-fractionation Homo sapiens
40 WWP2 11060
Affinity Capture-MS Homo sapiens
41 HIF1AN 55662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 B3GAT1  
Affinity Capture-MS Homo sapiens
43 FIS1 51024
Affinity Capture-MS Homo sapiens
44 C3orf18  
Affinity Capture-MS Homo sapiens
45 RPA3 6119
Proximity Label-MS Homo sapiens
46 MYH6 4624
Affinity Capture-MS Homo sapiens
47 Elavl1 15568
Affinity Capture-MS Mus musculus
48 ARRB1 408
Affinity Capture-MS Homo sapiens
49 XYLT1 64131
Affinity Capture-MS Homo sapiens
50 CMTM5  
Affinity Capture-MS Homo sapiens
51 GOLGA1  
Proximity Label-MS Homo sapiens
52 HSPA1A 3303
Affinity Capture-MS Homo sapiens
53 DHX34 9704
Affinity Capture-MS Homo sapiens
54 Cdc6 23834
Affinity Capture-MS Mus musculus
55 JUNB  
Reconstituted Complex Homo sapiens
56 NCS1 23413
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 SUV39H1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 BMX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 FAM168A  
Affinity Capture-MS Homo sapiens
60 FOXK1 221937
Co-fractionation Homo sapiens
61 GSDMB  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
62 HMGA1 3159
Affinity Capture-MS Homo sapiens
63 SLC33A1 9197
Affinity Capture-MS Homo sapiens
64 CDC5L 988
Affinity Capture-MS Homo sapiens
65 PPIF 10105
Cross-Linking-MS (XL-MS) Homo sapiens
66 RHOJ 57381
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 HYPM  
Affinity Capture-MS Homo sapiens
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