Gene description for MAPKAPK5
Gene name mitogen-activated protein kinase-activated protein kinase 5
Gene symbol MAPKAPK5
Other names/aliases MAPKAP-K5
MK-5
MK5
PRAK
Species Homo sapiens
 Database cross references - MAPKAPK5
ExoCarta ExoCarta_8550
Entrez Gene 8550
HGNC 6889
MIM 606723
UniProt Q8IW41  
 MAPKAPK5 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for MAPKAPK5
Molecular Function
    ATP binding GO:0005524 IEA
    protein serine/threonine kinase activity GO:0004674 IDA
    mitogen-activated protein kinase binding GO:0051019 IBA
    signal transducer activity GO:0004871 IBA
    calmodulin-dependent protein kinase activity GO:0004683 IBA
    calmodulin binding GO:0005516 IBA
    protein binding GO:0005515 IPI
    calcium-dependent protein serine/threonine kinase activity GO:0009931 IBA
    p53 binding GO:0002039 IDA
    MAP kinase kinase activity GO:0004708 TAS
Biological Process
    cell surface receptor signaling pathway GO:0007166 IBA
    MAPK cascade GO:0000165 TAS
    stress-induced premature senescence GO:0090400 IDA
    peptidyl-serine phosphorylation GO:0018105 IBA
    activation of MAPK activity GO:0000187 TAS
    negative regulation of TOR signaling GO:0032007 ISS
    protein autophosphorylation GO:0046777 IDA
    Ras protein signal transduction GO:0007265 IDA
    signal transduction GO:0007165 TAS
    regulation of translation GO:0006417 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified MAPKAPK5 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for MAPKAPK5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSPB2  
Biochemical Activity Homo sapiens
2 HSPB1 3315
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which MAPKAPK5 is involved
PathwayEvidenceSource
Oxidative Stress Induced Senescence TAS Reactome





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