Gene description for MTM1
Gene name myotubularin 1
Gene symbol MTM1
Other names/aliases CNM
MTMX
XLMTM
Species Homo sapiens
 Database cross references - MTM1
ExoCarta ExoCarta_4534
Vesiclepedia VP_4534
Entrez Gene 4534
HGNC 7448
MIM 300415
UniProt Q13496  
 MTM1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for MTM1
Molecular Function
    phosphatidylinositol-3-phosphate phosphatase activity GO:0004438 IBA
    phosphatidylinositol-3-phosphate phosphatase activity GO:0004438 IDA
    phosphatidylinositol-3-phosphate phosphatase activity GO:0004438 TAS
    phosphoprotein phosphatase activity GO:0004721 IDA
    protein binding GO:0005515 IPI
    intermediate filament binding GO:0019215 IDA
    phosphatidylinositol binding GO:0035091 IDA
    phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity GO:0052629 IBA
    phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity GO:0052629 IDA
    phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity GO:0052629 TAS
Biological Process
    autophagosome assembly GO:0000045 IEA
    protein dephosphorylation GO:0006470 IDA
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    mitochondrion organization GO:0007005 IDA
    endosome to lysosome transport GO:0008333 IDA
    protein transport GO:0015031 IEA
    TOR signaling GO:0031929 IEA
    negative regulation of TOR signaling GO:0032007 IEA
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IEA
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 IEA
    regulation of vacuole organization GO:0044088 IDA
    intermediate filament organization GO:0045109 IMP
    muscle cell cellular homeostasis GO:0046716 IBA
    phosphatidylinositol dephosphorylation GO:0046856 IBA
    phosphatidylinositol dephosphorylation GO:0046856 IDA
    mitochondrion distribution GO:0048311 IBA
    mitochondrion distribution GO:0048311 IMP
    skeletal muscle tissue growth GO:0048630 IEA
    positive regulation of skeletal muscle tissue growth GO:0048633 IEA
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IEA
    negative regulation of autophagosome assembly GO:1902902 IBA
Subcellular Localization
    ruffle GO:0001726 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    late endosome GO:0005770 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 IBA
    filopodium GO:0030175 IDA
    I band GO:0031674 IEA
 Experiment description of studies that identified MTM1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MTM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 IMMT 10989
Affinity Capture-MS Homo sapiens
3 OXA1L  
Affinity Capture-MS Homo sapiens
4 SNX27 81609
Affinity Capture-MS Homo sapiens
5 CGA  
Affinity Capture-MS Homo sapiens
6 KMT2A  
Reconstituted Complex Homo sapiens
7 CIAO1 9391
Affinity Capture-MS Homo sapiens
8 NDUFA9 4704
Affinity Capture-MS Homo sapiens
9 STOML2 30968
Affinity Capture-MS Homo sapiens
10 CALU 813
Affinity Capture-MS Homo sapiens
11 CCT8L1P  
Affinity Capture-MS Homo sapiens
12 TRIML2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 SAMM50 25813
Affinity Capture-MS Homo sapiens
14 MTMR11  
Affinity Capture-MS Homo sapiens
15 MTFR1  
Affinity Capture-MS Homo sapiens
16 KBTBD7  
Affinity Capture-MS Homo sapiens
17 SNX6 58533
Affinity Capture-MS Homo sapiens
18 RCN1 5954
Affinity Capture-MS Homo sapiens
19 VBP1 7411
Affinity Capture-MS Homo sapiens
20 MTMR10 54893
Affinity Capture-MS Homo sapiens
21 POTEF 728378
Affinity Capture-MS Homo sapiens
22 SOAT1 6646
Affinity Capture-MS Homo sapiens
23 SNX17 9784
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 TUBA3C 7278
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 PTPN9 5780
Affinity Capture-MS Homo sapiens
26 TARBP2  
Affinity Capture-MS Homo sapiens
27 AKR7A2 8574
Affinity Capture-MS Homo sapiens
28 KIAA1468 57614
Affinity Capture-MS Homo sapiens
29 KRT2 3849
Affinity Capture-MS Homo sapiens
30 ILK 3611
Affinity Capture-MS Homo sapiens
31 MTM1 4534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 KRT5 3852
Affinity Capture-MS Homo sapiens
33 STIP1 10963
Affinity Capture-MS Homo sapiens
34 BIN1 274
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
35 CUL4A 8451
Co-fractionation Homo sapiens
36 SCO2  
Affinity Capture-MS Homo sapiens
37 HLA-C 3107
Affinity Capture-MS Homo sapiens
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