Gene description for PAFAH2
Gene name platelet-activating factor acetylhydrolase 2, 40kDa
Gene symbol PAFAH2
Other names/aliases HSD-PLA2
Species Homo sapiens
 Database cross references - PAFAH2
ExoCarta ExoCarta_5051
Vesiclepedia VP_5051
Entrez Gene 5051
HGNC 8579
MIM 602344
UniProt Q99487  
 PAFAH2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PAFAH2
Molecular Function
    1-alkyl-2-acetylglycerophosphocholine esterase activity GO:0003847 IBA
    1-alkyl-2-acetylglycerophosphocholine esterase activity GO:0003847 ISS
    1-alkyl-2-acetylglycerophosphocholine esterase activity GO:0003847 TAS
    phospholipid binding GO:0005543 TAS
    platelet-activating factor acetyltransferase activity GO:0047179 ISS
Biological Process
    lipid metabolic process GO:0006629 TAS
    blood coagulation GO:0007596 TAS
    lipid catabolic process GO:0016042 IEA
Subcellular Localization
    cytoplasm GO:0005737 ISS
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified PAFAH2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PAFAH2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SSBP1 6742
Co-fractionation Homo sapiens
2 YWHAE 7531
Co-fractionation Homo sapiens
3 FH 2271
Co-fractionation Homo sapiens
4 AGTRAP 57085
Affinity Capture-MS Homo sapiens
5 PFDN4 5203
Co-fractionation Homo sapiens
6 DIABLO 56616
Co-fractionation Homo sapiens
7 ALDH2 217
Co-fractionation Homo sapiens
8 MRPL51 51258
Affinity Capture-MS Homo sapiens
9 HES4  
Affinity Capture-MS Homo sapiens
10 KIF3A 11127
Affinity Capture-MS Homo sapiens
11 GRPEL1 80273
Co-fractionation Homo sapiens
12 CTU2 348180
Affinity Capture-MS Homo sapiens
13 UBA1 7317
Co-fractionation Homo sapiens
14 USP28 57646
Affinity Capture-MS Homo sapiens
15 IARS2 55699
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which PAFAH2 is involved
PathwayEvidenceSource
Hemostasis TAS Reactome
Platelet homeostasis TAS Reactome





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