ExoCarta: Gene summary

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Gene description for SPC25
Gene name	SPC25, NDC80 kinetochore complex component
Gene symbol	SPC25
Other names/aliases	AD024 SPBC25 hSpc25
Species	Homo sapiens
Database cross references - SPC25
ExoCarta	ExoCarta_57405
Vesiclepedia	VP_57405
Entrez Gene	57405
HGNC	24031
MIM	609395
UniProt	Q9HBM1
SPC25 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells	36408942

Gene ontology annotations for SPC25

Molecular Function
	protein binding	GO:0005515	IPI
Biological Process
	mitotic spindle organization	GO:0007052	IMP
	chromosome segregation	GO:0007059	IBA
	chromosome segregation	GO:0007059	IMP
	chromosome segregation	GO:0007059	NAS
	mitotic spindle assembly checkpoint signaling	GO:0007094	NAS
	attachment of spindle microtubules to kinetochore	GO:0008608	IDA
	cell division	GO:0051301	IEA
Subcellular Localization
	kinetochore	GO:0000776	IDA
	kinetochore	GO:0000776	NAS
	outer kinetochore	GO:0000940	IDA
	nucleus	GO:0005634	IEA
	cytosol	GO:0005829	IDA
	cytosol	GO:0005829	TAS
	Ndc80 complex	GO:0031262	IBA
	Ndc80 complex	GO:0031262	IDA
	Ndc80 complex	GO:0031262	IPI

Experiment description of studies that identified SPC25 in exosomes

Experiment ID

488

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD81\|CD63\|GAPDH\|SDCBP\|LAMP1\|TFRC\|UCHL1\|FLOT2\|LAMP2\|FLOT1\|ICAM1\|RAB5B\|CD151\|RAB35\|TSG101\|RAB5A\|CD82	Enriched markers
✔ CANX	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Homo sapiens

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Mesenchymal stem cells

Sample name

UCMSC

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for SPC25

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

DSN1

79980

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

DNAJC7

7266

Affinity Capture-MS

Homo sapiens

MIS12

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RIT1

6016

Negative Genetic

Homo sapiens

ZWINT

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NUF2

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FAM170A

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

C12orf43

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

TNKS1BP1

85456

Co-fractionation

Homo sapiens

Ccdc12

Affinity Capture-MS

Mus musculus

IFT20

90410

Affinity Capture-MS

Homo sapiens

BUB1

Affinity Capture-MS

Homo sapiens

LMAN1

3998

Proximity Label-MS

Homo sapiens

LTBR

4055

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

S100A6

6277

Affinity Capture-MS

Homo sapiens

NSL1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

S100A2

6273

Affinity Capture-MS

Homo sapiens

SPC24

147841

Two-hybrid		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Two-hybrid		Homo sapiens
Affinity Capture-MS		Homo sapiens

EPS15

2060

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

Ndc80

Affinity Capture-MS

Mus musculus

CSPP1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

TKT

7086

Affinity Capture-MS

Homo sapiens

BOLA2

552900

Affinity Capture-MS

Homo sapiens

S100P

6286

Affinity Capture-MS

Homo sapiens

PMF1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PLEKHA4

57664

Affinity Capture-MS

Homo sapiens

LRPPRC

10128

Affinity Capture-MS

Homo sapiens

HSPA4

3308

Affinity Capture-MS

Homo sapiens

C6orf141

Affinity Capture-MS

Homo sapiens

GCC2

9648

Affinity Capture-MS

Homo sapiens

Casc5

Affinity Capture-MS

Mus musculus

HDGFRP2

84717

Affinity Capture-MS

Homo sapiens

S100A4

6275

Affinity Capture-MS

Homo sapiens

ARMCX1

51309

Cross-Linking-MS (XL-MS)

Homo sapiens

HNRNPH3

3189

Co-fractionation

Homo sapiens

Mis12

Affinity Capture-MS		Mus musculus
Affinity Capture-MS		Mus musculus

EFNA4

Affinity Capture-MS

Homo sapiens

AP2A1

160

Affinity Capture-MS

Homo sapiens

HGS

9146

Two-hybrid

Homo sapiens

CASC5

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NDC80

10403

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PSMD9

5715

Affinity Capture-MS

Homo sapiens

Bub1b

12236

Affinity Capture-MS

Mus musculus

AAMDC

Affinity Capture-MS

Homo sapiens

KTN1

3895

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SPECC1L

23384

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

METTL17

Affinity Capture-MS

Homo sapiens

Bub1

Affinity Capture-MS

Mus musculus

FCHO2

115548

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

EPS15L1

58513

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ATG16L1

55054

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

KIF2B

84643

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SEC61B

10952

Affinity Capture-Western

Homo sapiens

MYO18A

399687

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CENPQ

Affinity Capture-MS

Homo sapiens

CARTPT

Affinity Capture-MS

Homo sapiens

SNAP29

9342

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

View the network

Pathways in which SPC25 is involved

Pathway	Evidence	Source
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal	TAS	Reactome
Amplification of signal from the kinetochores	TAS	Reactome
Cell Cycle	TAS	Reactome
Cell Cycle Checkpoints	TAS	Reactome
Cell Cycle, Mitotic	TAS	Reactome
EML4 and NUDC in mitotic spindle formation	TAS	Reactome
M Phase	TAS	Reactome
Mitotic Anaphase	TAS	Reactome
Mitotic Metaphase and Anaphase	TAS	Reactome
Mitotic Prometaphase	TAS	Reactome
Mitotic Spindle Checkpoint	TAS	Reactome
Resolution of Sister Chromatid Cohesion	TAS	Reactome
RHO GTPase Effectors	TAS	Reactome
RHO GTPases Activate Formins	TAS	Reactome
Separation of Sister Chromatids	TAS	Reactome
Signal Transduction	TAS	Reactome
Signaling by Rho GTPases	TAS	Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here