Gene description for SPC25
Gene name SPC25, NDC80 kinetochore complex component
Gene symbol SPC25
Other names/aliases AD024
SPBC25
hSpc25
Species Homo sapiens
 Database cross references - SPC25
ExoCarta ExoCarta_57405
Vesiclepedia VP_57405
Entrez Gene 57405
HGNC 24031
MIM 609395
UniProt Q9HBM1  
 SPC25 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SPC25
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    cell division GO:0051301 IEA
    small GTPase mediated signal transduction GO:0007264 TAS
    mitotic spindle organization GO:0007052 IMP
    mitotic cell cycle GO:0000278 TAS
    mitotic nuclear division GO:0007067 IEA
    chromosome segregation GO:0007059 IMP
Subcellular Localization
    Ndc80 complex GO:0031262 IDA
    condensed chromosome kinetochore GO:0000777 IDA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IEA
 Experiment description of studies that identified SPC25 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SPC25
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC61B 10952
Invivo Homo sapiens
2 NDC80 10403
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 MIS12  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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